PDE11A antibody

Principal name

PDE11A antibody

Alternative names for PDE11A antibody

Phosphodiesterase 11A, PDE11A1, PDE11A2, PDE11A3, PDE11A4, cAMP and cGMP phosphodiesterase 11A, Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A

SwissProt ID

P0C1Q2 (Mouse), Q8VID6 (Rat), Q9HCR9 (Human), Q9VJ79 (Drome)

Gene ID

50940 (PDE11A)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Can (Canine)

Available hosts

Rabbit, Goat

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C)

Background of PDE11A antibody

Cyclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. Recently a new and distinct member (PDE11) of cyclic nucleotide family has been identified and cloned (1). The PDE11A exhibit 50% amino acid homology with the catalytic domain of the all other PDEs. The PDE11A resembles most to the PDE5 family members but has distinct biochemical properties than PDE5. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP and cGMP in to AMP and GMP respectively are phosphodiesterase type 11. The human PDE11A contains an open reading frame encoding a 490 amino acid protein (55-56 kDa). The PDE11A has an N-terminus GAF domain homologous to other signaling molecule as found in PDE2, PDE5, PDE6, PDE10 this potentially represent an allosteric cGMP and other small signaling molecules. cyclic nucleotide phosphodiesterase (PDE)11A, which exhibits properties of a dual-substrate PDE, and its splice variants (RNPDE11A2, RNPDE11A3, and RNPDE11A4). The deduced amino-acid sequence of the longest form of rat PDE11A splice variant, A4, was 94% identical with that of the human variant (A4). The Km for for cAMP and cGMP were similar for all PDE11 variants (1.3-1.6 and 2.1-3.9 microM, PDE11A2, A3 and A4, respectively), the Vmax values differed significantly or rat PDE11A (A4>>A2>>A3). Human PDE11A variants also displayed very similar Km values and significantly different Vmax values (A4>>A2>>A3). The Vmax values of HSPDE11A4 for cAMP and cGMP were at least 100 times higher than those of HSPDE11A1. These observations indicate unique characteristics of PDE11A splicing variants (2).
Rat PDE11A splice variants were expressed in a tissue-specific manner. RNPDE11A4 showed unique tissue distribution distinct from HSPDE11A4, which is specifically expressed in the prostate. The PDE11A is sensitive to non-selective PDE inhibitor IBMX as well as zaprinast and dipyrimadole, inhibitors that are generally selective for cGMP-specific enzymes. PDE11A expression is diverse and is found in high levels in skeletal muscle, prostate, kidney, liver, pituitary and testis. At least three variants that may occur in vivo based on the three transcripts identified in various tissues ranging in size from 10.5 kb, 8.5 and 6.0 kb. PDE11A4 is composed of 934 amino acids including two complete GAF domains and shared 630 C-terminal amino acids with PDE11A3 but had a distinct N terminus containing the putative phosphorylation sites for cAMP- and cGMP-dependent protein kinases (3).

General readings

Horvath A, Boikos S, Giatzakis C, Robinson-White A, Groussin L, Griffin KJ, Stein E, Levine E, Delimpasi G, Hsiao HP, Keil M, Heyerdahl S, Matyakhina L, Libe R, Fratticci A, Kirschner LS, Cramer K, Gaillard RC, Bertagna X, Carney JA, Bertherat J, Bossis I, Stratakis CA. A genome-wide scan identifies mutations in the gene encoding phosphodiesterase 11A4 (PDE11A) in individuals with adrenocortical hyperplasia. Nat Genet. 2006 Jul;38(7):794-800. Epub 2006 Jun 11. PMID: 16767104

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE11A antibody

Anti-PDE11A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

PDE11A antibody

TA303261 (1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE11A antibody

Figure 1: Western blot using anti-PDE11A antibody (1:5,000) against lysate from Insect cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE11A (455-468) antibody

Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PDE11A Control Peptide

PDE11A
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE11A (N-term) antibody

Western blot with AP10259PU-N (lane 1) Rabbit Aff - Purified Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE11A control peptide

PDE11A
0.1 mg / €250.00
  Acris Antibodies GmbH

PDE11A antibody

PDE11A Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE11A control peptide

PDE11A
0.1 mg / €250.00
  Acris Antibodies GmbH

PDE11A antibody

PDE11A polyclonal antibody ( Cat # PAB7327 ) ( 1 µg/ml ) staining of human prostate lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PDE11A antibody

PDE11A Rabbit Hu, Ms, Rt C, IP, WB
50 µl / €560.00
  Abnova Taiwan Corp.

PDE11A antibody

PDE11A Antibody Rabbit Serum Hu, Ms, Rt IP, P, WB
50 µl / €400.00
  Immuquest

PDE11A antibody

NB100-61669 (1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PDE11A antibody

Western Blot: PDE11A Antibody [NBP1-32910] - Western blot analysis of PDE11A on Hep G2 lysate using NBP1-32910 at a  dilution of 1:5000. 30ug of whole cell lysate loaded on 5% SDS PAGE gel. Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

PDE11A antibody

Immunohistochemistry-Paraffin: PDE11A Antibody [NBP1-85919] - Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

PDE11A4 (isoform specific) antibody

PDE11A4 Rabbit Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE11A (transcript variant 4)

PDE11A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

PDE11A overexpression lysate

PDE11A overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

18 Item(s)

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