PDE1A antibody

Principal name

PDE1A antibody

Alternative names for PDE1A antibody

Phosphodiesterase 1A, Phosphodiesterase-1A calmodulin-dependent, Cam-PDE 1A, HSPDE1A, HCAM1, hCam-1, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A, 61 kDa Cam-PDE

SwissProt ID

P14100 (Bovin), P54750 (Human), Q61481 (Mouse), Q9C0L4 (Human)

Gene ID

5136 (PDE1A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of PDE1A antibody

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The PDE1A gene has been cloned from human, mouse and rat. The PDE1A gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein. There are three novel PDE1A variants so far identified (PDE1A1; PDE1A3 and PD1A5, pde1a7), PDE1A5 encodes a 519 residue protein , which is different from PDE1A1 by insertion of 14 amino acid residues at the carboxy terminal and is also different from PDE1A3 by a divergent at N-terminus (Miller and Lanfear, 2002). The predicted size of PDE1A7 gene product is a 55kDa protein. Members of the PDE1A gene have a longer carboxy terminus that accounts for their larger size. The tissue distribution of PDE1A7 is largely in testis and differs from other PDE1 gene family members. In testis PDE1A7 are highly expressed but indifferent stages of in developing Germ cells. The PDE1A7 mRNA is also found in round to elongated spermatids, with protein expression in in the tails of elongated and maturing spermatids (Yan et. al., 2001).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE1A antibody

  PDE1A mouse monoclonal antibody, clone OTI7D5 (formerly 7D5)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PDE1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803629) Mouse IgG2a OTI7D5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE1A antibody

  Rabbit Polyclonal antibody to PDE1A (phosphodiesterase 1A, calmodulin-dependent)  
Sample (30 ug of whole cell lysate). A: H1299. B: Hela. 7.5% SDS PAGE. TA308379 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDE1A antibody

  Goat Anti-PDE1A Antibody  
(1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE1A antibody

  Rabbit Polyclonal Anti-PDE1A Antibody  
WB Suggested Anti-PDE1A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE1A antibody

  PDE1A mouse monoclonal antibody, clone OTI5C9 (formerly 5C9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1A (RC205292, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1A. Mouse IgG1 OTI5C9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE1A antibody

  PDE1A mouse monoclonal antibody, clone OTI5H4 (formerly 5H4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1A (RC205292, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1A. Mouse IgG2b OTI5H4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE1A antibody

Figure 1: Western blot using anti-PDE1A antibody (1:20,000) against lysate from COS cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PDE1A - human gene knockout kit via CRISPR

PDE1A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PDE1A - human gene knockout kit via CRISPR

PDE1A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDE1A - human gene knockout kit via CRISPR

PDE1A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDE1A - human gene knockout kit via CRISPR

PDE1A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE1A (transcript variant 1)

  Recombinant protein of human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1  
PDE1A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PDE1A overexpression lysate

PDE1A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PDE1A (mGFP-tagged) - Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE1A (mGFP-tagged) - Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE1A (mGFP-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE1A (mGFP-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,160.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE1A (Myc-DDK tagged) - Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE1A (Myc-DDK tagged) - Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE1A (Myc-DDK-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE1A (Myc-DDK-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,040.00
  OriGene Technologies, Inc.

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