PDE1B antibody

Principal name

PDE1B antibody

Alternative names for PDE1B antibody

Phosphodiesterase 1B, Phosphodiesterase-1B calmodulin-dependent, Cam-PDE 1B, PDE1B1, PDES1B, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, 63 kDa Cam-PDE

SwissProt ID

Q01061 (Bovin), Q01064 (Human), Q01065 (Mouse), Q01066 (Rat)

Gene ID

5153 (PDE1B), 18574 (Pde1b), 29691

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PDE1B antibody

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The PDE1B gene has been cloned from human, mouse and rat. The PDE1B gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein. There are at least two novel PDE1B variants so far identified (PDE1B1 and PDE1B2), PDE1B2 encodes a 516 residue protein, which is different from PDE1B1 by replacement of the first 38 residues by an alternative 18 which is predicted to be functionally significant as far its expression in various tissues. (Miller and Lanfear, 2002). The predicted size of PDE1B gene products ranged between 65-68 kDa. Members of the PDE1B gene have a longer carboxy terminus that accounts for their larger size. The tissue distribution of PDE1B is different from other PDE1 gene family members. In testis PDE1B is expressed uniformly through out the seminiferous epithelium and in the interstistium in contrast to PDE1A and PDE1C that is expressed in elongated to mature spermatids (Yan et. al., 2001).

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Vandeput, F., et al. J. Biol. Chem. 282(45):32749-32757(2007)
Bender, A.T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(2):460-465(2006)
Bender, A.T., et al. Proc. Natl. Acad. Sci. U.S.A. 102(2):497-502(2005)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE1B antibody

  Rabbit anti-PDE1B Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PDE1B antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE1B antibody

  PDE1B mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503319) Mouse IgG2b OTI6E4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE1B antibody

  PDE1B mouse monoclonal antibody, clone OTI6B9 (formerly 6B9)  
Anti-PDE1B mouse monoclonal antibody (TA503325) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B(RC206588). Mouse IgG2b OTI6B9 Purified Can, Hu, Mky, Rt F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PDE1B antibody

  Anti-PDE1B Rabbit Polyclonal Antibody  
Predicted band size: 61 kDa . Positive control: NCL and THP-1 cell, mouse brain and spleen tissue, spinal cord tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: NCL cell lysate Lane2: THP-1 cell lysate Lane3: Mouse brain tissue lysate Lane4: Mouse spleen tissue lysate Lane5: Spinal cord tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDE1B antibody

  Rabbit Polyclonal Anti-PDE1B Antibody  
WB Suggested Anti-PDE1B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:2500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE1B antibody

  PDE1B mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)  
Anti-PDE1B mouse monoclonal antibody (TA503336) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B(RC206588). Mouse IgG2b OTI1D6 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE1B (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with PDE1B Antibody (C-term) Cat.-No AP53233PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDE1B (C-term) antibody

Western blot analysis of PDE1B Antibody (C-term) Cat.-No AP53234PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the PDE1B antibody detected the PDE1B protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Pde1b - mouse gene knockout kit via CRISPR

Pde1b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pde1b - mouse gene knockout kit via CRISPR

Pde1b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pde1b - mouse gene knockout kit via CRISPR

Pde1b - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pde1b - mouse gene knockout kit via CRISPR

Pde1b - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE1B

  Recombinant protein of human phosphodiesterase 1B, calmodulin-dependent (PDE1B)  
PDE1B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PDE1B (mGFP-tagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE1B (mGFP-tagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE1B (mGFP-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE1B (mGFP-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,140.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE1B (Myc-DDK tagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE1B (Myc-DDK tagged) - Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE1B (Myc-DDK-tagged)-Human phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,020.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pde1b (GFP-tagged) - Mouse phosphodiesterase 1B, Ca2+-calmodulin dependent (cDNA clone MGC:67093 IMAGE:6826674), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pde1b (GFP-tagged) - Mouse phosphodiesterase 1B, Ca2+-calmodulin dependent (cDNA clone MGC:67093 IMAGE:6826674), 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.

Lenti ORF particles, Pde1b (GFP-tagged) - Mouse phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pde1b (GFP-tagged) - Mouse phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), 200 uL, >10^7 TU/mL  
200 µl / €1,160.00
  OriGene Technologies, Inc.

Lenti ORF particles, Pde1b (Myc-DDK-tagged) - Mouse phosphodiesterase 1B, Ca2+-calmodulin dependent (cDNA clone MGC:67093 IMAGE:6826674), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pde1b (Myc-DDK-tagged) - Mouse phosphodiesterase 1B, Ca2+-calmodulin dependent (cDNA clone MGC:67093 IMAGE:6826674), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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