PDE1C antibody

Principal name

PDE1C antibody

Alternative names for PDE1C antibody

Phosphodiesterase 1C, Phosphodiesterase-1C calmodulin-dependent, HCAM3, hCam-3, Cam-PDE 1C, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C

SwissProt ID

Q14123 (Human), Q63421 (Rat), Q64338 (Mouse)

Gene ID

5137 (PDE1C)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), African clawed frog, Eq (Equine), Hst (Hamster), Mky (Monkey), Rb (Rabbit), Gorilla, Por (Porcine), Ze (Zebrafish), GP (Guinea Pig)

Available hosts

Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of PDE1C antibody

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, Pde1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The PDE1C gene has been cloned from human, mouse and rat. The PDE1C gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein. The predicted size of PDE1C gene products ranged between 70-87 kDa. Members of the PDE1C gene have a longer carboxy terminus that accounts for their larger size. The tissue distribution of PDE1C is different from other PDE1 gene family members. A 5’ splice variant of PDE1C2 is found in rat olfactory epithelium (Yan and Beavo 1994; Yan et. al., 1995) and is believed to be involved in olfactory signal transduction. Other PDE1C variants (PDE1C1 and PDE1C2) are found in human heart (Loughney et. al., 1996). The PDE1C enzymes bind both cGMP and cAMP with different affinities. The phosphodiesterase type-1C has at least three variants (PDE1C1, PDE1C2, PDE1C3) ranging in molecular weight between 70-87 kDa. PDE1C is 634 amino acids long and has at least three splice variants that are expressed in heart, olfactory epithelium and other brain regions, lungs and testis.

General readings

General / background references:Loughney K et al. Isolation and characterization of cDNAs corresponding to two human calcium, calmodulin-regulated, 3',5'-cyclic nucleotide phosphodiesterases. J Biol Chem 271:796-806 (1996). PubMed PMID: 8557689
Yan C et al. Molecular cloning and characterization of a calmodulin-dependent phosphodiesterase enriched in olfactory sensory neurons. Proc Natl Acad Sci U S A 92:9677-81 (1995). PubMed PMID: 7568196
Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991). PubMed PMID: 1832709

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE1C (C-term) antibody

Anti-PDE1C antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Ms, Rt P
50 µg / €345.00
  OriGene Technologies, Inc.

PDE1C antibody

WB Suggested Anti-PDE1C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE1C antibody

WB Suggested Anti-PDE1C Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE1C Control Peptide

PDE1C
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE1C antibody

PDE1C Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE1C antibody

Human HepG2; WB Suggested Anti-PDE1C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; PDE1C antibody - N-terminal region (ARP53563_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PDE1C antibody

Human HepG2; WB Suggested Anti-PDE1C Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; PDE1C antibody - middle region (ARP56615_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PDE1C antibody

PDE1C Rabbit Aff - Purified Hu P
50 µg / €440.00
  Novus Biologicals Inc.

PDE1C antibody

Western Blot: PDE1C Antibody [NB300-672] - Western blot using NB300-672 (1:500) and 1. Brain; 2. Heart 3. Lung; 4. Testis and 5. Kidney extracts. Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE1C (N-term HIS tag, transcript variant 4)

PDE1C Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

10 Item(s)

per page
  • LinkedIn