PDE3A antibody

Principal name

PDE3A antibody

Alternative names for PDE3A antibody

Phosphodiesterase 3A, Phosphodiesterase-3A cGMP-inhibited, CGI-PDE, CGI-PDE A, cGMP-inhibited 3',5'-cyclic phosphodiesterase A, Cyclic GMP-inhibited phosphodiesterase A

SwissProt ID

Q14432 (Human), Q62865 (Rat), Q9Z0X4 (Mouse)

Gene ID

5139 (PDE3A)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Por (Porcine), Mky (Monkey), Eq (Equine), Bov (Bovine)

Available hosts

Goat, Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C)

Background of PDE3A antibody

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. The members of the PDE families are known as cGMP inhibited PDEs. The PDE3 enzymes bind both cGMP and cAMP with different affinities (cAMP 4-10-fold than cGMP). The phosphodiesterase type-3 (PDE3) family is comprised of 2 genes (PDE3 A and B). PDE3A is a 125 kDa protein and is expressed in various tissues (myocardium, vascular and non vascular smooth muscles, megakaryocytes and a subset of neurons (Snyder 1999; Maecci et al., 1992). PDE3A has two splice variants and are expressed in adipocyte tissue, hepatocyte, kidney, spermatocytes and embryonic neuroepithelium (Reinhatdt et. al., 1995). Both PDE3A and PDE3B are therapeutic targets for the treatment of obesity as inhibition of PDE3 increase the lipolytic activity and alter the insulin secretion and its response. PDE3 inhibitors also promote thermogenic and cardiotonic effects in animals.

General readings

General / background references:Meacci E et al. Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase. Proc Natl Acad Sci U S A 89:3721-5 (1992). PubMed PMID: 1315035
Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991). PubMed PMID: 1832709

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE3A (N-term) antibody

Anti-PDE3A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €345.00
  OriGene Technologies, Inc.

PDE3A (N-term) antibody

Anti-PDE3A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €345.00
  OriGene Technologies, Inc.

PDE3A antibody

Anti-PDE3A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Ms, Rt P
50 µg / €345.00
  OriGene Technologies, Inc.

PDE3A antibody

WB Suggested Anti-PDE3A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE3A antibody

Western blot using AP00653SU-N (1:20,000) against lysate from COS cells transfected with the indicated human PDE isoform Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE3A Control Peptide

PDE3A
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE3A antibody

PDE3A Rabbit Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE3A antibody

PDE3A Goat Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE3A antibody

PDE3A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PDE3A antibody

Detection limit for recombinant GST tagged PDE3A is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 5C12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDE3A antibody

PDE3A Mouse IgG2b 2D7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDE3A antibody

PDE3A Rabbit Hu IP
0.1 mg / €560.00
  Abnova Taiwan Corp.

PDE3A antibody

PDE3A Rabbit Hu, Ms, Rt C, IP, WB
50 µl / €560.00
  Abnova Taiwan Corp.

PDE3A antibody

Human MCF-7; WB Suggested Anti-PDE3A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate; PDE3A antibody - N-terminal region (ARP44356_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Chk, Hu, Por WB
50 µg / €350.00
  AVIVA Systems Biology

PDE3A antibody

PDE3A Antibody Rabbit Serum Hu, Ms, Rt IP, P, WB
50 µl / €400.00
  Immuquest

PDE3A antibody

Western Blot: PDE3A Antibody [NB300-673] - Western blot detection of PDE3A at an antibody dilution of 1:250. Goat Aff - Purified Can, Hu, Ms, Rt IP, WB
0.1 mg / €530.00
  Novus Biologicals Inc.

PDE3A antibody

Western Blot: PDE3A Antibody [NBP1-46181]-Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Affinity purified rabbit anti-PDE3A antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  PDE3A was also immunoprecipitated by rabbit anti-PDE3A antibody NBP1-46182, which recognizes a downstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PDE3A antibody

Western Blot: PDE3A Antibody [NBP1-46182]-Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-PDE3A antibody  used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  PDE3A was also immunoprecipitated by rabbit anti-PDE3A antibody NBP1-46181, which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE3A

PDE3A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PDE3A

PDE3A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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