PDE3B antibody

Principal name

PDE3B antibody

Alternative names for PDE3B antibody

Phosphodiesterase 3B, Phosphodiesterase-3B cGMP-inhibited, HcGIP1, CGIPDE1, CGI-PDE B, CGIP, CGIP1, cGMP-inhibited 3',5'-cyclic phosphodiesterase B, Cyclic GMP-inhibited phosphodiesterase B

SwissProt ID

Q13370 (Human), Q61409 (Mouse), Q63085 (Rat)

Gene ID

5140 (PDE3B), 18576 (Pde3b)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of PDE3B antibody

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. The members of the PDE3 families are known as cGMP inhibited PDEs. The PDE3 enzymes bind both cGMP and cAMP with different affinities (cAMP 4-10-fold than cGMP). The phosphodiesterase type-3 (PDE3) family is comprised of 2 genes (PDE3A and PDE3B). PDE3A is a 145 kDa protein and is expressed in various tissues (myocardium, vascular and non vascular smooth muscles, megakaryocytes and a subset of neurons (Snyder 1999; Maecci et al., 1992). PDE3B is 1112 amino acids long and has two splice variants which are expressed in adipocyte tissue, hepatocyte, kidney, spermatocytes and embryonic neuroepithelium (Reinhatdt et. al., 1995). Both PDE3A and PDE3B are therapeutic targets for the treatment of obesity as inhibition of PDE3 increase the lipolytic activity and alter the insulin secretion and its response. PDE3 inhibitors also promote thermogenic and cardiotonic effects in animals.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Yamano, Y., et al. Int. J. Cancer 126(2):437-449(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Hanson, M.S., et al. Am. J. Physiol. Heart Circ. Physiol. 295 (2), H786-H793 (2008)
Raymond, D.R., et al. Cell. Signal. 19(12):2507-2518(2007)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE3B (Phospho) antibody

PDE3B Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

PDE3B antibody

Western blot analysis of PDE3B with lysate from 3T3 cells. Rabbit Purified Hu, Ms, Rt WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

PDE3B antibody

Lysates prepared from mouse heart (lane 1) or lymph node cells (lane 2) were run under reducing conditions on a 3-8% Tris-Acetate gel. The membrane was probed with 2 ug/mL of Anti-Mouse PDE3B Purified, followed by Anti-Rat IgG HRP. Rat SMCP3B Purified Ms WB
25 µg / €150.00
  OriGene Technologies, Inc.

PDE3B antibody

WB Suggested Anti-PDE3B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE3B antibody

Western blot using AP00654SU-N (1:20,000) against lysate from COS cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE3B Control Peptide

PDE3B
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE3B antibody

PDE3B Rabbit Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE3B antibody

PDE3B Goat Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE3B Control Peptide

PDE3B
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE3B (Center) antibody

Confocal immunofluorescent analysis of PDE3B Antibody (Center) Cat.-No AP53235PU-N with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Aff - Purified Hu F, ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDE3B antibody

PDE3B Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PDE3B antibody

Detection limit for recombinant GST tagged PDE3B is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 4A4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDE3B antibody

Human 293T; WB Suggested Anti-PDE3B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate; PDE3B antibody - middle region (ARP44359_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PDE3B antibody

PDE3B Antibody Rabbit Serum Hu, Ms, Rt IP, P, WB
50 µl / €410.00
  Immuquest

PDE3B antibody

PDE3B Rat IgG2a SMCP3B Aff - Purified Ms WB
0.1 mg / €470.00
  Novus Biologicals Inc.

PDE3B antibody

PDE3B Rat IgG2a SMCP3B Aff - Purified Ms WB
25 µg / €340.00
  Novus Biologicals Inc.

PDE3B antibody

Western Blot: PDE3B Antibody [NBP1-46183] - Detection of Human PDE3B by Western Blot and_x000d_
Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE3B antibody NBP1-46183  used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B)_x000d_
and used for IP at 3 mcg/mg lysate. PDE3B was also_x000d_
immunoprecipitated by rabbit anti-PDE3B antibody NBP1-46184,_x000d_
which recognizes a downstream epitope. For blotting_x000d_
immunoprecipitated PDE3B, the blot  Reagents and_x000d_
Procedures were used. Detection:_x000d_
Chemiluminescence with exposure times of 3 minutes (A) and 30_x000d_
seconds (B). Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

PDE3B antibody

Western Blot: PDE3B Antibody [NBP1-46184] - Detection of Human PDE3B by Western Blot and_x000d_
Immunoprecipitation. Samples: Whole cell lysate (5, 15 and50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDE3B antibody_x000d_
NBP1-46184 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B)_x000d_
and used for IP at 3 mcg/mg lysate. PDE3B was also_x000d_
immunoprecipitated by rabbit anti-PDE3B antibody A302-742A,_x000d_
which recognizes an upstream epitope. For blotting_x000d_
immunoprecipitated PDE3B, the blot Reagents and_x000d_
Procedures (Cat. No. WB120) were used. Detection:_x000d_
Chemiluminescence with exposure times of 30 seconds(A) and_x000d_
10 seconds (B). Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE3B

PDE3B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PDE3B

PDE3B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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