PDE4A antibody

Principal name

PDE4A antibody

Alternative names for PDE4A antibody

Phosphodiesterase 4A, Phosphodiesterase-4A cAMP-specific, PDE46, PDE4, DPDE2, PDE4A4, PDE4A4B, PDE4A11, PDE4A7, PDE4A6, PDE4A1, RD1, PDE4A8A, 2EL, PDE4A10, PDE4A8, cAMP-specific 3',5'-cyclic phosphodiesterase 4A

SwissProt ID

O89084 (Mouse), O89085 (Cavpo), P27815 (Human), P54748 (Rat), Q8WUQ3 (Human), Q9W4T4 (Drome)

Gene ID

5141 (PDE4A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P)

Background of PDE4A antibody

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes (1). Members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling (6, 7). In testis PDE4A variants are expressed exclusively in primary and secondary germ cells and are believed to be responsible for normal spermatogenesis (8). PDE4 enzymes are also important molecular targets for variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprised of 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants (2). Rolipram, an antidepressant drug, inhibits all the members of PDE4 family in mM concentration range. In rodents, inhibition of PDE4 enzymes attenuated short- and long-term memory impairment produced by scopolamine and MK801 administration (3). Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases (4). Two conserved phosphorylation motifs have been identified in PDE4A subtype variants (i) Phosphorylation at PKA site and (ii) an ERK kinase site. Phosphorylation at PKA site resulted in significant increase I enzymatic activity of PDE4A variants. Phosphorylation state, protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling (5).

General readings

General / background references:Zhang HT et al. Inhibition of cyclic AMP phosphodiesterase (PDE4) reverses memory deficits associated with NMDA receptor antagonism. Neuropsychopharmacology 23:198-204 (2000). PubMed PMID: 10882846
Yarwood SJ et al. The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform. J Biol Chem 274:14909-17 (1999). PubMed PMID: 10329691
Beavo JA Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol Rev 75:725-48 (1995). PubMed PMID: 7480160
Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991). PubMed PMID: 1832709

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE4A antibody

Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody (TA501152). Mouse IgG2a OTI1E3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501153, Dilution 1:50) Mouse IgG2b OTI1F5 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501192, Dilution 1:50) Mouse IgG1 OTI1C8 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501198, Dilution 1:50) Mouse IgG1 OTI3D4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501199, Dilution 1:50) Mouse IgG1 OTI7D11 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501200, Dilution 1:50) Mouse IgG1 OTI2G2 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501201, Dilution 1:50) Mouse IgG2b OTI7B11 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501202, Dilution 1:50) Mouse IgG1 OTI6B6 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501203, Dilution 1:50) Mouse IgG1 OTI6D6 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501206, Dilution 1:50) Mouse IgG2b OTI5C6 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE4A  mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501207, Dilution 1:50) Mouse IgG1 OTI8B7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4A antibody

Western blot using AP00655SU-N (1:2,000) against lysate from COS cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE4A control peptide

PDE4A
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE4A (C-term) antibody

PDE4A Rabbit Aff - Purified Chk, Hu, Mky, Ms, Rt E, ICC/IF, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE4A control peptide

PDE4A
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE4A (phosphospecific) antibody

PDE4A Rabbit Aff - Purified Chk, Hu, Mky, Ms, Rt E, F, ICC/IF, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE4A control peptide

PDE4A
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE4A antibody

Western Blot analysis of PDE4A expression in transfected 293T cell line (H00005141-T01) by PDE4A MaxPab polyclonal antibody.

Lane 1: PDE4A transfected lysate(71.17 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PDE4A antibody

Western Blot analysis of PDE4A expression in transfected 293T cell line (H00005141-T01) by PDE4A MaxPab polyclonal antibody.

Lane 1: PDE4A transfected lysate(71.17 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PDE4A antibody

PDE4A Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Items 1 to 20 of 43 total

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