PDE4B antibody

Principal name

PDE4B antibody

Alternative names for PDE4B antibody

PDE4B, DPDE4, cAMP-specific 3',5'-cyclic phosphodiesterase 4B, EC=3.1.4.17, PDE32

SwissProt ID

P14646 (Rat), Q07343 (Human)

Gene ID

5142 (PDE4B), 24626 (Pde4b)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey), Bov (Bovine), Por (Porcine), African clawed frog, Hst (Hamster), Prim (Primate), Rb (Rabbit), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of PDE4B antibody

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. Four genes (PDE4A, B, C and D) make up the PDE4 family. cAMP-dependent phosphodiesterase type B (PDE4B) family is consists of 4 known splice variants, PDE4B1, B2, B3 and a novel 66 kDa PDE4B4 protein (1, 2). One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In CNS, all four PDE4B subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Some PDE4B variants are also substrate for PKA-dependent phosphorylation. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants; the PDE4B4 (66kDa protein) is the major PDE4B variant expressed in lungs, testis and heart. In testis PDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4A is localized in germ cells (2).
PDE4B2 variant (b) contains a distinct 5’ UTR and lacks an in-frame portion of the 5’ coding region, compared to variant a. The resulting isoform, PDE4B2 (PDE32) has a shorter and distinct N-terminus when compared to isoform 1. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The PDE4B2 expression is found in brain, heart, lung and skeletal muscle but not in placenta, liver, kidney or pancrease.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Souza, R.P., et al. Int Clin Psychopharmacol 25(5):264-269(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE4B antibody

  PDE4B mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503439) Mouse IgG2b OTI2B6 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4B antibody

  PDE4B mouse monoclonal antibody, clone OTI1D12 (formerly 1D12)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503471) Mouse IgG1 OTI1D12 Purified Hu, Ms F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PDE4B antibody

  PDE4B mouse monoclonal antibody, clone OTI3B12 (formerly 3B12)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503477) Mouse IgG2b OTI3B12 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4B antibody

  PDE4B mouse monoclonal antibody, clone OTI3A10 (formerly 3A10)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503532) Mouse IgG1 OTI3A10 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4B (C-term) antibody

AP31948PU-N PDE4B antibody staining of Human Uterus lysate at 2 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PDE4B antibody

  Goat Polyclonal Antibody against Phosphodiesterase 4B  
TA302571 (0.5µg/ml) staining of Mouse Brain Lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE4B antibody

  Rabbit polyclonal antibody to Phosphodiesterase 4B (phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila))  
Sample (30 ug of whole cell lysate) . A: NT2D1. B:  PC-3. 7.5% SDS PAGE . TA308931 diluted at 1:1000.   Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDE4B antibody

  Rabbit Polyclonal Anti-PDE4B Antibody  
WB Suggested Anti-PDE4B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE4B antibody

  PDE4B mouse monoclonal antibody, clone OTI2B10 (formerly 2B10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B. Mouse IgG1 OTI2B10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4B antibody

  PDE4B mouse monoclonal antibody, clone OTI1B11 (formerly 1B11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B. Mouse IgG1 OTI1B11 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE4B antibody

Western blot using Scottish AP00656SU-N (1:2,000) against lysate from COS cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE4B (723-736) antibody

Immunohistochemistry Image: Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Hst, Hu, Mky, Ms, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PDE4B (Center) antibody

Western blot analysis of PDE4B Antibody (Center) Cat.-No AP53236PU-N in Ramos cell line lysates (35ug/lane). This demonstrates the PDE4B antibody detected the PDE4B protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDE4B cAMP- antibody

PDE4B Rabbit Purified Hu C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE4B (transcript variant d)

  Recombinant protein of human phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila) (PDE4B), transcript variant d  
PDE4B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PDE4B (transcript variant a)

  Recombinant protein of human phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila) (PDE4B), transcript variant a  
PDE4B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

PDE4B overexpression lysate

PDE4B overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PDE4B (mGFP-tagged) - Human phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant a, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE4B (mGFP-tagged) - Human phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant a, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE4B (mGFP-tagged) - Human phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant b, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE4B (mGFP-tagged) - Human phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant b, 200 uL, >10^7 TU/mL  
200 µl / €1,210.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE4B (mGFP-tagged)-Human phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant c, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE4B (mGFP-tagged)-Human phosphodiesterase 4B, cAMP-specific (PDE4B), transcript variant c, 200 uL, >10^7 TU/mL  
200 µl / €1,600.00
  OriGene Technologies, Inc.

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