PDE4D antibody

Principal name

PDE4D antibody

Alternative names for PDE4D antibody

PDE4D, DPDE3, cAMP-specific 3',5'-cyclic phosphodiesterase 4D, EC=3.1.4.17, DPDE3, PDE43

SwissProt ID

P14270 (Rat), Q01063 (Mouse), Q08499 (Human), Q9W4T0 (Drome)

Gene ID

5144 (PDE4D), 238871 (Pde4d), 24627

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey), Bt (Bat), Bov (Bovine), Prim (Primate), Rb (Rabbit), Eq (Equine), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of PDE4D antibody

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes (1). Members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling (6, 7).
ERK mitogen activated protein kinase and cAMP pathways are important regulators for cross talk between protein kinases and cAMP at distinct points in the cell (2). The phosphodiesterase type 4b, C and D sbtypes, but not the PDE4A subtype, have a consensus ERK site phosphorylation site. This site in PDE4D3 lies at serine at position 579 (3-5). The ERK kinase phosphorylation site lies in the consensus motif Pro-Xaa-Ser-Pro. In vivo, a KIM motif (Val-Xaa-Xaa-Lys-Lys-Xaa6-Leu-Leu-Leu-Xaa12-Phospho-serine) serves as a docking site for ERK/JNK and is located upstream to the target phosphorylation site. The specificty for ERK is confered over JNK by the presence of FQF motif down stream from the phosphorylation site (6). PDE4B, C and D all have conserved KIM and FQF sites that provide serine as substrate for ERK phosphorylation. The crystal structure and the three dimentional modeling of the PDE4 D/B suggest that both KIM and FQF domain are located on a single microdoamin, the KIM being located on exposed hair pin loop and the FQF on an exposed a helix (6). The location of these doamins excelently displaced for ERK internations.
The phosphorylation of PDE4B, C, D long-form variants by ERK kinases led to a significant inhibition (approximately 80%) in PDEase activity (3-5). ERK phosphorylation of PDE4 long-forms cause an increse in cAMP as a resultof inhibition in PDEase activity. The rise in cAMP levels consequentially activated the cAMP-dependent PKA that phosphorylates the same protiens at PKA site (serine 53 in PDE4D3) that led to a significant increase in PDEase activity (7). Taken together these effects will generate a traanssient increase in cAMP lervels in a cytosolic sub-compartment.

General readings

Houslay, M.D., Baillie, G.S. and Maurice, D.H.
cAMP-Specific phosphodiesterase-4 enzymes in the cardiovascular system: a molecular toolbox for generating compartmentalized cAMP signaling.
Circ Res. 2007 Apr 13;100(7):950-66. Review.
PMID: 17431197

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE4D antibody

  Goat Polyclonal Antibody against PDE4D  
TA302564 staining (1µg/ml) of COS cell lysates (25µg protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE4D (Isoform 3) (N-term) antibody

  Goat Polyclonal Antibody against PDE4D3  
TA302565 (0.01µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

  Rabbit Polyclonal antibody to PDE4D (phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila))  
Sample (30 ug of whole cell lysate). A: A431. B: HeLa. 7.5% SDS PAGE. TA308932 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

  Rabbit anti-PDE4D Polyclonal Antibody  
Western blot analysis of extracts of mouse braincell lines , using PDE4D antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

  Rabbit Polyclonal Anti-PDE4D Antibody - C-terminal region  
Host: Rabbit; Target Name: PDE4D; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

  Rabbit Polyclonal Anti-PDE4D Antibody - N-terminal region  
WB Suggested Anti-Pde4d Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Mouse Brain Rabbit IgG Purified Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

  Rabbit Polyclonal Anti-PDE4D Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Human bladder carcinoma tissue, A172 cells, human fetal brain tissue, hela cells, Primary antibody: (PDE4D Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

  Rabbit Polyclonal Anti-PDE4D Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Hela cells, Primary antibody: (PDE4D Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

Western blot using AP00657SU-N (1:2,000) against lysate from COS cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE4D antibody

Immunohistochemistry analysis of PDE4D antibody (Cat.-No.: AP06558PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PDE4D (796-809) antibody

Immunohistochemistry Image:  
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Bt, Hu, Mky, Prim, Rb E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PDE4D pSer190/53 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PDE4D (Phospho-Ser190/53) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

PDE4D pSer190/53 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PDE4D (Phospho-Ser190/53) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

PDE4D antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PDE4D (Ab-190/53) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

PDE4D antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PDE4D (Ab-190/53) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Pde4d - mouse gene knockout kit via CRISPR

Pde4d - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pde4d - mouse gene knockout kit via CRISPR

Pde4d - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pde4d - mouse gene knockout kit via CRISPR

Pde4d - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pde4d - mouse gene knockout kit via CRISPR

Pde4d - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE4D (transcript variant 2)

  Recombinant protein of human phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (PDE4D), transcript variant 2  
PDE4D Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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