PDE4D antibody

Principal name

PDE4D antibody

Alternative names for PDE4D antibody

PDE4D, DPDE3, cAMP-specific 3',5'-cyclic phosphodiesterase 4D, EC=3.1.4.17, DPDE3, PDE43

SwissProt ID

P14270 (Rat), Q01063 (Mouse), Q08499 (Human), Q9W4T0 (Drome)

Gene ID

5144 (PDE4D), 9659 (PDE4DIP), 238871 (Pde4d), 24627, 5144724 (mpg)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Chk (Chicken), Mky (Monkey), Por (Porcine), Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P), Dot blot (Dot), Flow Cytometry (F)

Background of PDE4D antibody

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes (1). Members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling (6, 7).
ERK mitogen activated protein kinase and cAMP pathways are important regulators for cross talk between protein kinases and cAMP at distinct points in the cell (2). The phosphodiesterase type 4b, C and D sbtypes, but not the PDE4A subtype, have a consensus ERK site phosphorylation site. This site in PDE4D3 lies at serine at position 579 (3-5). The ERK kinase phosphorylation site lies in the consensus motif Pro-Xaa-Ser-Pro. In vivo, a KIM motif (Val-Xaa-Xaa-Lys-Lys-Xaa6-Leu-Leu-Leu-Xaa12-Phospho-serine) serves as a docking site for ERK/JNK and is located upstream to the target phosphorylation site. The specificty for ERK is confered over JNK by the presence of FQF motif down stream from the phosphorylation site (6). PDE4B, C and D all have conserved KIM and FQF sites that provide serine as substrate for ERK phosphorylation. The crystal structure and the three dimentional modeling of the PDE4 D/B suggest that both KIM and FQF domain are located on a single microdoamin, the KIM being located on exposed hair pin loop and the FQF on an exposed a helix (6). The location of these doamins excelently displaced for ERK internations.
The phosphorylation of PDE4B, C, D long-form variants by ERK kinases led to a significant inhibition (approximately 80%) in PDEase activity (3-5). ERK phosphorylation of PDE4 long-forms cause an increse in cAMP as a resultof inhibition in PDEase activity. The rise in cAMP levels consequentially activated the cAMP-dependent PKA that phosphorylates the same protiens at PKA site (serine 53 in PDE4D3) that led to a significant increase in PDEase activity (7). Taken together these effects will generate a traanssient increase in cAMP lervels in a cytosolic sub-compartment.

General readings

General / background references:Hoffmann R et al. The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579. EMBO J 18:893-903 (1999). PubMed PMID: 10022832
Erdogan S & Houslay MD Challenge of human Jurkat T-cells with the adenylate cyclase activator forskolin elicits major changes in cAMP phosphodiesterase (PDE) expression by up-regulating PDE3 and inducing PDE4D1 and PDE4D2 splice variants as well as down-regulating a novel PDE4A splice variant. Biochem J 321 ( Pt 1):165-75 (1997). PubMed PMID: 9003416
Bolger GB et al. Characterization of five different proteins produced by alternatively spliced mRNAs from the human cAMP-specific phosphodiesterase PDE4D gene. Biochem J 328 ( Pt 2):539-48 (1997). PubMed PMID: 9371713
Alvarez R et al. Activation and selective inhibition of a cyclic AMP-specific phosphodiesterase, PDE-4D3. Mol Pharmacol 48:616-22 (1995). PubMed PMID: 7476886

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE4D antibody

TA302564 staining (1µg/ml) of COS cell lysates (25µg protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE4D (Isoform 3) (N-term) antibody

TA302565 (0.01µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

Sample (30 ug of whole cell lysate). A: A431. B: HeLa. 7.5% SDS PAGE. TA308932 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

Western blot analysis of extracts of mouse braincell lines , using PDE4D antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

Host: Rabbit; Target Name: PDE4D; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

WB Suggested Anti-Pde4d Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Mouse Brain Rabbit IgG Purified Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE4D antibody

Western blot using AP00657SU-N (1:2,000) against lysate from COS cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE4D antibody

Immunohistochemistry analysis of PDE4D antibody (Cat.-No.: AP06558PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PDE4D control peptide

PDE4D
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE4D (C-term) (D1-D5) antibody

PDE4D Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE4D control peptide

PDE4D
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE4D (phosphospecific) antibody

PDE4D Rabbit Aff - Purified Chk, Hu, Mky, Ms, Rt F, ICC/IF, IP, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

PDE4D pSer579 antibody

PDE4D Rabbit Aff - Purified Chk, Hu, Mky, Ms, Rt WB
0.1 mg / €490.00
  Acris Antibodies GmbH

PDE4D (796-809) antibody

Immunohistochemistry Image:  
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Bt, Hu, Mky, Prim, Rb E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PDE4D antibody

Immunofluorescence of monoclonal antibody to PDE4DIP on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2B5 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDE4D antibody

HEK293 overexpressing human PDE4D2 and probed with PDE4D polyclonal antibody ( Cat # PAB6924 ) ( mock transfection in first lane ), tested by Origene. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PDE4D antibody

PDE4D polyclonal antibody ( Cat # PAB7166 ) ( 0.01 µg/ml ) staining of rat brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PDE4D antibody

PDE4D Rabbit Hu, Ms, Rt C, IP, WB
50 µl / €560.00
  Abnova Taiwan Corp.

PDE4D pSer190/53 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PDE4D (Phospho-Ser190/53) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

PDE4D pSer190/53 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PDE4D (Phospho-Ser190/53) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

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