PDE5A antibody

Principal name

PDE5A antibody

Alternative names for PDE5A antibody

PDE5, cGMP-specific 3',5'-cyclic phosphodiesterase, EC=3.1.4.35, cGMP-binding cGMP-specific phosphodiesterase, CGB-PDE

SwissProt ID

O76074 (Human)

Gene ID

8654 (PDE5A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of PDE5A antibody

The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-PDEs). The cGB-PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. The cGMP-specific phosphodiesterase type-5A (PDE5A) family is comprised of single gene with multiple splice variants (PDE5A1 and PDE5A2) generated by RNA splicing and use of alternate initiation sites (1, 2). The PDE5 is highly expressed in aorta and lungs, intestine, kidney adrenal gland cerebellum and cerebrum. In cerebellum the PDE5 is highly expressed during neonatal development in Purkinge cells layer. The PDE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of PDE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate PDE5 activity by cGMP-dependent kinase phosphorylation (Rybalkin et. al., 2002; Murthy 2001). PDE5A1 has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of the PDE5 gene showed no sequence homology with other PDEs and also contained serine 92 that is phosphorylated by cGMP-kinases (McAllister et al., 93).

General readings

Morelli A, Filippi S, Mancina R, Luconi M, Vignozzi L, Marini M, Orlando C, Vannelli GB, Aversa A, Natali A, Forti G, Giorgi M, Jannini EA, Ledda F, Maggi M. Androgens regulate phosphodiesterase type 5 expression and functional activity in corpora cavernosa. Endocrinology. 2004 May;145(5):2253-63.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE5A (C-term) antibody

TA302566 staining (0.3µg/ml) of human lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDE5A antibody

Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (PDE5A Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDE5A antibody

Western blot using AP00658SU-N (1/2,000) against lysate from COS cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, P, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE5A Control Peptide

PDE5A
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE5A antibody

Figure 2. Immunostaining of cells expressing PDE5 with this antibody Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE5A control peptide

PDE5A
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE5A (N-term) antibody

PDE5A Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE5A antibody

PDE5A Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

PDE5A antibody

PDE5A Mouse IgG3 9H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDE5A antibody

PDE5A Mouse IgG3 1F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDE5A antibody

HEK293 overexpressing PDE5A and probed with PDE5A polyclonal antibody ( Cat # PAB6741 ) ( mock transfection in first lane ), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PDE5A antibody

PDE5A Rabbit Hu, Ms, Rt C, IP, WB
50 µl / €560.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE5A (transcript variant 2)

PDE5A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PDE5A

PDE5A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PDE5A

PDE5A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PDE5A overexpression lysate

PDE5A overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

PDE5A overexpression lysate

PDE5A overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PDE5A (mGFP-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE5A (mGFP-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE5A (mGFP-tagged) - Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE5A (mGFP-tagged) - Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,780.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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