PDE6A antibody

Principal name

PDE6A antibody

Alternative names for PDE6A antibody

Phosphodiesterase 6A, Phosphodiesterase-6A cGMP-specific, PDE6 alpha, PDEA, GMP-PDE alpha, PDE V-B1, Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

SwissProt ID

P11541 (Bovin), P16499 (Human), P27664 (Mouse), Q28263 (Canfa)

Gene ID

5145 (PDE6A)

Available reactivities

Bov (Bovine), Sh (Sheep), Hu (Human), Ms (Mouse), Rt (Rat), Hst (Hamster), Mky (Monkey), Rb (Rabbit), Gorilla

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PDE6A antibody

The retinal cone and rod photoreceptor cells can directly stimulate and response by photons of the light. Upon light stimulation these cells undergo a highly regulated cascade of events that leads to changes in the photoreceptor membrane potentials and subsequent neurotransmitter release. The key component of photo transduction in retina is retinal specific cGMP-dependent phosphodiesterases type 6 (PDE6s). These PDEs convert cGMP in to GMP when activated by the G-protein transducin and caused a closer of the cGMP gated ion channels, reduced Na+ and Ca++ influx and hyperpolarization of the photoreceptor cells. Rodhopsin and PDE6 are also colocalized in the internal membraneous disk of the photoreceptor cells allowing most efficient transfer of light signal from one protein to other. The retinal phosphodiesterses are multimeric proteins consisting of catalytic and inhibitory subunits. The large alfa and beta (a and b) subunits of the rod and cone specific PDE6 dimerises to form the catalytic complex of the PDE6 isozyme. The smaller gamma subunit serves as an inhibitor for the rod and cone phosphodiesterses (1). There are 3 subtypes of PDE6 found in rod and cone cells, PDE6a, PDE6b and and inhibitory PDE6g. The rod and cone PDEs differ significantly in subunit compositions, sensitivity to transducin, and cGP binding properties (2). The PDE6a and beta forms have 2 non catalytic binding cGMP sites; the cone biding site has significantly lower Ki for cGMP that the rod enzymes. There is significant homology between a and b subunits in the catalytic and GAF binding domains.
Both PDE6a and PDE6b are post-translationally modified, PDE6a is farnsylated and beta subunit is gernylgernylated (3). Generally, these modifications will allow proteins to be anchored to the membranes, incase of PDE6, despite 2 lipid modifications the PDE can easily be removed form membranes by detergent free hypotonic buffers. The PDE6 can be localized in both membrane and cytosolic compartment, the cytosolic PDE6 has been show to be associated with a 17 kDa delta subunit (4). The role of delta subunit in PDE6 functioning is not fully understood. It been hypothesized that delta subunit might be playing a role in PDE6 solubility in the photoreceptor cells. The PDE6a is a 103-105 kDa protein (858 amino acids) with 2 GAF and one PDEase catalytic domain.

General readings

General / background references:He F et al. Multiple zinc binding sites in retinal rod cGMP phosphodiesterase, PDE6alpha beta. J Biol Chem 275:20572-7 (2000). PubMed PMID: 10787404
Granovsky AE & Artemyev NO Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '. J Biol Chem 275:41258-62 (2000). PubMed PMID: 11024033

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE6A (N-term) antibody

Anti-PDE6A / PDE6 Alpha antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €345.00
  OriGene Technologies, Inc.

PDE6A antibody

Host: Rabbit; Target Name: PDE6A; Sample Tissue: Fetal Thymus lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE6A Control Peptide

PDE6A
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE6A antibody

PDE6A Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE6A antibody

PDE6A Rabbit Aff - Purified Hu P
50 µg / €440.00
  Novus Biologicals Inc.

PDE6A antibody

Western Blot: PDE6 Antibody [NB120-5660] - Western blot of PDE6 alpha on sheep retinal extracts using NB120-5660. Rabbit IgG Bov, Sh WB
0.1 ml / €400.00
  Novus Biologicals Inc.

PDE6A antibody

Immunohistochemistry-Paraffin: PDE6A Antibody [NBP1-87312] Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

PDE6A overexpression lysate

PDE6A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

8 Item(s)

per page
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