PDE6B antibody

Principal name

PDE6B antibody

Alternative names for PDE6B antibody

PDEB, Phosphodiesterase 6B, Phosphodiesterase-6B cGMP-specific, Phosphodiesterase 6 beta, GMP-PDE beta, PDE6 beta, Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

SwissProt ID

P23439 (Bovin), P23440 (Mouse), P33726 (Canfa), P35913 (Human)

Gene ID

5158 (PDE6B), 18587 (Pde6b), 100065158 (SPI2)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PDE6B antibody

The retinal cone and rod photoreceptor cells can directly stimulate and response by photons of the light. Upon light stimulation these cells undergo a highly regulated cascade of events that leads to changes in the photoreceptor membrane potentials and subsequent neurotransmitter release. The key component of photo transduction in retina is retinal specific cGMP-dependent phosphodiesterases type 6 (PDE6s). These PDEs convert cGMP in to GMP when activated by the G-protein transducin and caused a closer of the cGMP gated ion channels, reduced Na+ and Ca++ influx and hyper polarization of the photoreceptor cells. Rodhopsin and PDE6 are also co-localized in the internal membraneous disk of the photoreceptor cells allowing most efficient transfer of light signal from one protein to other. The retinal phosphodiesterses are multimeric proteins consisting of catalytic and inhibitory subunits. The large alfa and beta (a and b) subunits of the rod and cone specific PDE6 dimerises to form the catalytic complex of the PDE6 isozyme. The smaller gamma subunit serves as an inhibitor for the rod and cone phosphodiesterses (1). There are 3 subtypes of PDE6 found in rod and cone cells, PDE6g PDE6b and an inhibitory PDE6g. The rod and cone PDEs differ significantly in subunit compositions, sensitivity to transducin, and cGP binding properties (2). The PDE6a and b forms have 2 non catalytic binding cGMP sites; the cone biding site has significantly lower Ki for cGMP that the rod enzymes. There is significant homology between a and b subunits in the catalytic and GAF binding domains.
Both PDE6a and PDE6 b are post-translationally modified, PDE6b is farnsylated and beta subunit is gernylgernylated (3). Generally, these modifications will allow proteins to be anchored to the membranes, incase of PDE6, despite 2 lipid modifications the PDE can easily be removed form membranes by detergent free hypotonic buffers. The PDE6 can be localized in both membrane and cytosolic compartment, the cytosolic PDE6 has been show to be associated with a 17 kDa delta subunit (4). The role of delta subunit in PDE6 functioning is not fully understood. It been hypothesized that delta subunit might be playing a role in PDE6 solubility in the photoreceptor cells. The PDE6b is a 103-105 kDa protein (858 amino acids) with 2 GAF and one PDEase catalytic domain.

General readings

He F et al. Multiple zinc binding sites in retinal rod cGMP phosphodiesterase, PDE6alpha beta. J Biol Chem 275:20572-7 (2000). PubMed PMID: 10787404
Granovsky AE & Artemyev NO Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '. J Biol Chem 275:41258-62 (2000). PubMed PMID: 11024033

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE6B Control Peptide

PDE6B
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE6B antibody

PDE6B Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE6B antibody

PDE6B Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

PDE6B antibody

Immunofluorescent: PDE6 Antibody [NB120-5663] - Figure 2 shows immunoflourences of mouse retina outer segments after staining with NB120-5663. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms ICC/IF, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

PDE6B overexpression lysate

PDE6B overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

5 Item(s)

per page
  • LinkedIn