PDE6C antibody

Principal name

PDE6C antibody

Alternative names for PDE6C antibody

Phosphodiesterase 6C, Phosphodiesterase-6C cGMP-specific, PDEA2, COD4, Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'

SwissProt ID

P16586 (Bovin), P51160 (Human), P52731 (Chick), Q91ZQ1 (Mouse)

Gene ID

5146 (PDE6C)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PDE6C antibody

The retinal cone and rod photoreceptor cells can directly stimulate and response by photons of the light. Upon light stimulation these cells undergo a highly regulated cascade of events that leads to changes in the photoreceptor membrane potentials and subsequent neurotransmitter release. The key component of photo transduction in retina is retinal specific cGMP-dependent phosphodiesterases type 6 (PDE6s). These PDEs convert cGMP in to GMP when activated by the G-protein transducin and caused a closer of the cGMP gated ion channels, reduced Na+ and Ca++ influx and hyper polarization of the photoreceptor cells. Rodhopsin and PDE6 are also co-localized in the internal membraneous disk of the photoreceptor cells allowing most efficient transfer of light signal from one protein to other. The retinal phosphodiesterses are multimeric proteins consisting of catalytic and inhibitory subunits. The large alfa and beta (ƒÑ and ƒÒ) subunits of the rod and cone specific PDE6 dimerises to form the catalytic complex of the PDE6 isozyme. The smaller gamma subunit serves as an inhibitor for the rod and cone phosphodiesterses (1). There are 3 subtypes of PDE6 found in rod and cone cells, PDE6ƒ× PDE6ƒÒ and an inhibitory PDE6ƒ×. The rod and cone PDEs differ significantly in subunit compositions, sensitivity to transducin, and cGP binding properties (2). The PDE6ƒÑ and ƒÒ forms have 2 non catalytic binding cGMP sites; the cone biding site has significantly lower Ki for cGMP that the rod enzymes. There is significant homology between a and b subunits in the catalytic and GAF binding domains.
Both PDE6ƒÑ and PDE6ƒnƒÒ are post-translationally modified, PDE6ƒÒ is farnsylated and beta subunit is gernylgernylated (3). Generally, these modifications will allow proteins to be anchored to the membranes, incase of PDE6, despite 2 lipid modifications the PDE can easily be removed form membranes by detergent free hypotonic buffers. The PDE6 can be localized in both membrane and cytosolic compartment, the cytosolic PDE6 has been show to be associated with a 17 kDa delta subunit (4). The role of delta subunit in PDE6 functioning is not fully understood. It been hypothesized that delta subunit might be playing a role in PDE6 solubility in the photoreceptor cells. The PDE6ƒÒ is a 103-105 kDa protein (858 amino acids) with 2 GAF and one PDEase catalytic domain.

General readings

Mustafi D et al. J Clin Invest. 2012 Aug 1;122(8):2989-3001. doi: 10.1172/JCI64427
Muradov, H., et al. J. Biol. Chem. 284(47):32662-32669(2009)
Chang, B., et al. Proc. Natl. Acad. Sci. U.S.A. 106(46):19581-19586(2009)
Thiadens, A.A., et al. Am. J. Hum. Genet. 85(2):240-247(2009)

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE6C control peptide

PDE6C
0.1 mg / €250.00
  Acris Antibodies GmbH

PDE6C (15-26) antibody

PDE6C Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE6C (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue reacted with PDE6C Antibody (Center) Cat.-No AP53238PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDE6C antibody

PDE6C polyclonal antibody (A01), Lot # 060116JC01 Western Blot analysis of PDE6C expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PDE6C antibody

PDE6C Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE6C

PDE6C Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PDE6C

PDE6C Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PDE6C overexpression lysate

PDE6C overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

8 Item(s)

per page
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