PDE6G antibody

Principal name

PDE6G antibody

Alternative names for PDE6G antibody

Phosphodiesterase 6G, Phosphodiesterase-6G cGMP-specific, PDEG, GMP-PDE gamma, PDE6 gamma, Phosphodiesterase 6 gamma, Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma

SwissProt ID

P04972 (Bovin), P09174 (Mouse), P18545 (Human), P54827 (Canfa), P61248 (Felca), Q9EQQ8 (Cavpo)

Gene ID

5148 (PDE6G), 281977

Available reactivities

Bov (Bovine), Sh (Sheep), Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)

Background of PDE6G antibody

The retinal cone and rod photoreceptor cells can directly stimulate and response by photons of the light. Upon light stimulation these cells undergo a highly regulated cascade of events that leads to changes in the photoreceptor membrane potentials and subsequent neurotransmitter release. The key component of photo transduction in retina is retinal specific cGMP-dependent phosphodiesterases type 6 (PDE6s). These PDEs convert cGMP in to GMP when activated by the G-protein transducin and caused a closer of the cGMP gated ion channels, reduced Na+ and Ca++ influx and hyper polarization of the photoreceptor cells. Rodhopsin and PDE6 are also co-localized in the internal membraneous disk of the photoreceptor cells allowing most efficient transfer of light signal from one protein to other. The retinal phosphodiesterses are multimeric proteins consisting of catalytic and inhibitory subunits. The large alfa and beta (a and b) subunits of the rod and cone specific PDE6 dimerizes to form the catalytic complex of the PDE6 isozyme. The smaller gamma subunit serves as an inhibitor for the rod and cone phosphodiesterses (1). There are 3 subtypes of PDE6 found in rod and cone cells, PDE6g PDE6b and an inhibitory PDE6g. The rod and cone PDEs differ significantly in subunit compositions, sensitivity to transducin, and cGP binding properties (2). The PDE6a and b forms have 2 non catalytic binding cGMP sites; the cone biding site has significantly lower Ki for cGMP that the rod enzymes. There is significant homology between a and b subunits in the catalytic and GAF binding domains.
Both PDE6a and PDE6 b are post-translationally modified, PDE6b is farnsylated and beta subunit is gernylgernylated (3). Generally, these modifications will allow proteins to be anchored to the membranes, incase of PDE6, despite 2 lipid modifications the PDE can easily be removed form membranes by detergent free hypotonic buffers. The PDE6 can be localized in both membrane and cytosolic compartment, the cytosolic PDE6 has been show to be associated with a 17 kDa delta subunit (4). The role of delta subunit in PDE6 functioning is not fully understood. It been hypothesized that delta subunit might be playing a role in PDE6 solubility in the photoreceptor cells. The PDE6b is a 103-105 kDa protein (858 amino acids) with 2 GAF and one PDEase catalytic domain. The inhibitory subunit (PDE6g) is a 87 amino acid peptide with an approximate molecular weight of 11kDa. The association of PDE6g subunit suppresses the PDE mediated cGMP hydrolysis.

General readings

General / background references:He F et al. Multiple zinc binding sites in retinal rod cGMP phosphodiesterase, PDE6alpha beta. J Biol Chem 275:20572-7 (2000). PubMed PMID: 10787404
Granovsky AE & Artemyev NO Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '. J Biol Chem 275:41258-62 (2000). PubMed PMID: 11024033

9 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE6G antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE6G (RC216236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE6G. Mouse IgG2a OTI1F3 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE6G antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE6G (RC216236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE6G. Mouse IgG2a OTI1H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE6G antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE6G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503736) Mouse IgG2b OTI2F5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDE6G antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE6G (RC216236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE6G. Mouse IgG1 OTI1E11 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PDE6G Control Peptide

PDE6G
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE6G antibody

PDE6G Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE6G antibody

Western Blot: PDE6 Antibody [NB120-5664] - Figure 1 shows a Western blot of PDE6 gamma on sheep retinal extracts using NB120-5664. Rabbit IgG Bov, Sh WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE6G (transcript variant 1)

PDE6G Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PDE6G overexpression lysate

PDE6G overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

9 Item(s)

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