PDE7A antibody

Principal name

PDE7A antibody

Alternative names for PDE7A antibody

Phosphodiesterase 7A, PDE7, HCP1, TM22, High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A

SwissProt ID

O08593 (Rat), P70453 (Mouse), Q13946 (Human)

Gene ID

5150 (PDE7A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Hst (Hamster), Mky (Monkey), Rb (Rabbit), Xen (Xenopus), Gorilla, Eq (Equine), GP (Guinea Pig), Por (Porcine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of PDE7A antibody

The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP in T-Lymphocytes are phosphodiesterase type 7. The cAMP-specific phosphodiesterase type-7 (PDE7) family is comprised of 2 genes (PDE7A and PDE7B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites (1). PDE7A has two splice variants (PDE7A1 and PDE7A2), the two are divergent at the 5’ end in human and PDE7A1 is more hydrophobic (2). Like other PDEs, human PDE7A gene has 456 amino acids and migrates at an apparent molecular weight of 53-55 kDa on reduced SDS-PAGE. The PDE7A has a significant conserved region of about 270 amino acids common to all PDEs at the carboxy terminal apparently serves as the catalytic domain. The amino-terminal region of this protein is divergent and presumably accounts for the distinctive and regulatory properties unique to the individual PDE families. PDE7A protein showed significant homology to other cAMP-dependent PDEs (23%) with in the catalytic domain. PDE7A is widely expressed in various tissues including skeletal muscle, T lymphocytes, brain, pancreas.

General readings

General / background references:Bloom TJ & Beavo JA Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc Natl Acad Sci U S A 93:14188-92 (1996). PubMed PMID: 8943082
Michaeli T et al. Isolation and characterization of a previously undetected human cAMP phosphodiesterase by complementation of cAMP phosphodiesterase-deficient Saccharomyces cerevisiae. J Biol Chem 268:12925-32 (1993). PubMed PMID: 8389765
Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991). PubMed PMID: 1832709

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE7A (N-term) antibody

Anti-PDE7A antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €345.00
  OriGene Technologies, Inc.

PDE7A antibody

WB Suggested Anti-PDE7A Antibody; Titration: 1.0 ug/ml; Positive Control: 721_B Whole Cell Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE7A antibody

Western blot using AP00659SU-N (1:2,000) against lysate from COS cells transfected with the indicated human PDE isoform. Rabbit Serum Hu, Ms, Rt C, IP, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

PDE7A Control Peptide

PDE7A
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE7A antibody

Figure 1. Western Blot using PDE7A antibody (AP10247PU-N):
Lane 1: Lungs 
Lane 2: Kidney
Lane 3: Testis 
Lane 4: Pancreas
Lane 5: Heart
Lane 6: Brain
Lane 7: Heart Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE7A antibody

PDE7A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PDE7A antibody

PDE7A Antibody Rabbit Serum Hu, Ms, Rt IP, P, WB
50 µl / €400.00
  Immuquest

PDE7A antibody

PDE7A Rabbit Aff - Purified Hu P
50 µg / €440.00
  Novus Biologicals Inc.

PDE7A antibody

Western Blot: PDE7A Antibody [NB300-639] - Western Blot with PDE7A 1. Lungs 2. Kidney 3. Testis 4. Pancrease 5. Heart 6. Brain 7. Heart Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

PDE7A antibody

Immunohistochemistry-Paraffin: PDE7A Antibody [NBP1-83275] Immunohistochemical staining of human heart muscle shows distinct cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE7A

PDE7A Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

PDE7A

PDE7A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

PDE7A

PDE7A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PDE7A overexpression lysate

PDE7A overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

14 Item(s)

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