PDE7B antibody

Principal name

PDE7B antibody

Alternative names for PDE7B antibody

Phosphodiesterase 7B, MGC88256, cAMP-specific 3',5'-cyclic phosphodiesterase 7B, rolipram-insensitive phosphodiesterase type 7

SwissProt ID

Q8VIE4 (Rat), Q9NP56 (Human), Q9QXQ1 (Mouse)

Gene ID

27115 (PDE7B), 140929 (Pde7b), 29863

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of PDE7B antibody

The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP in T-Lymphocytes are phosphodiesterase type 7. The cAMP-specific phosphodiesterase type-7 (PDE7) family is comprised of 2 genes (PDE7A and PDE7B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites (1). PDE7B has three splice variants (PDE7B1 = 446 amino acid; PDE7B2 =359 amino acids and PDE7B3 = 459 amino acids. Both PDE7B2 ad PDE7B3 posses unique N-terminal sequences.
Like other PDEs, human PDE7B gene products have an apparent molecular weight of 53-55 kDa on reduced SDS-PAGE (2). As in PDE7A, the PDE7B has a significant conserved region of about 270 amino acids common to all PDEs at the carboxy terminal apparently serves as the catalytic domain. The amino-terminal region of this protein is divergent and presumably accounts for the distinctive and regulatory properties unique to the individual PDE families. PDE7B protein showed significant homology to other cAMP-dependent PDEs (23%) with in the catalytic domain. PDE7B is expressed in various tissues including skeletal muscle, heart, lung and testis (2).

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Kestenbaum, B., et al. J. Am. Soc. Nephrol. 21(7):1223-1232(2010)
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Ikeda, M., et al. Biol. Psychiatry 67(3):263-269(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE7B (N-term) antibody

  Phosphodiesterase 7b / PDE7B Rabbit Polyclonal (N-Terminus) Antibody  
Anti-PDE7B antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €400.00
  OriGene Technologies, Inc.

PDE7B antibody

  Phosphodiesterase 7b / PDE7B Rabbit Polyclonal (Internal) Antibody  
Anti-PDE7B antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €400.00
  OriGene Technologies, Inc.

PDE7B (C-term) antibody

  Phosphodiesterase 7b / PDE7B Rabbit Polyclonal (C-Terminus) Antibody  
Anti-PDE7B antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €400.00
  OriGene Technologies, Inc.

PDE7B antibody

  Rabbit Polyclonal Anti-PDE7B Antibody  
WB Suggested Anti-PDE7B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE7B Control Peptide

PDE7B
0.1 mg / €270.00
  Acris Antibodies GmbH

PDE7B antibody

Western blot with AP10248PU-N 
(1:1000). Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE7B Control Peptide

PDE7B
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE7B antibody

PDE7B Rabbit Aff - Purified Hu IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE7B (C-term) antibody

Western blot analysis of PDE7B Antibody (C-term) Cat.-No AP53239PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the PDE7B antibody detected the PDE7B protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PDE7B - human gene knockout kit via CRISPR

PDE7B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PDE7B - human gene knockout kit via CRISPR

PDE7B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDE7B - human gene knockout kit via CRISPR

PDE7B - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDE7B - human gene knockout kit via CRISPR

PDE7B - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE7B

  Recombinant protein of human phosphodiesterase 7B (PDE7B)  
PDE7B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

PDE7B overexpression lysate

PDE7B overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PDE7B (mGFP-tagged) - Human phosphodiesterase 7B (PDE7B), 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE7B (mGFP-tagged) - Human phosphodiesterase 7B (PDE7B), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDE7B (Myc-DDK tagged) - Human phosphodiesterase 7B (PDE7B) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PDE7B (Myc-DDK tagged) - Human phosphodiesterase 7B (PDE7B) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pde7b (GFP-tagged) - Mouse phosphodiesterase 7B (Pde7b), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pde7b (GFP-tagged) - Mouse phosphodiesterase 7B (Pde7b), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, Pde7b (Myc-DDK-tagged) - Mouse phosphodiesterase 7B (Pde7b), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pde7b (Myc-DDK-tagged) - Mouse phosphodiesterase 7B (Pde7b), 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Pde7b (GFP-tagged ORF) - Rat phosphodiesterase 7B (Pde7b), 200 uL, >10^7 TU/mL

Lenti ORF particles, Pde7b (GFP-tagged ORF) - Rat phosphodiesterase 7B (Pde7b), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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