PDE8A antibody

Principal name

PDE8A antibody

Alternative names for PDE8A antibody

Phosphodiesterase 8A, FLJ16150, HsT19550, High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A, cAMP-specific cyclic nucleotide phosphodiesterase 8A

SwissProt ID

O60658 (Human), O88502 (Mouse), Q9UMB7 (Human)

Gene ID

5151 (PDE8A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit), Gorilla, Bov (Bovine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PDE8A antibody

Cyclic nucleotides are important intracellular second messengers which play important role variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cAMP in to AMP are phosphodiesterase type 8. The cAMP-specific phosphodiesterase type-8 (PDE8) family is comprised of 2 genes (PDE8A and PDE8B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites (1). PDE8 family is a high affinity cAMP-specific, IBMX sensitive PDE. Like other PDEs, human PDE8A has 713 amino acids and migrate at apparent 100 kDa on reduced and non-reduced SDS-PAGE. The PDE8 has a significant conserved region of about 270 amino acids common to all PDEs at the carboxy-terminal apparently serves as the catalytic domain. The amino-terminal region of this protein is divergent and presumably accounts for the distinctive and regulatory properties unique to the individual PDE families. PDE8A protein showed significant homology to other cAMP-dependent PDEs (23%) with in the catalytic domain. PDE8A is widely expressed in various tissues in contrast to PDE8B that is exclusively expressed in thyroid gland. The PDE8A transcripts are found in brain, pancreas, placenta, thyroid, spleen, trachea, prostate, and uterus.

General readings

General / background references:Hayashi M et al. Molecular cloning and characterization of human PDE8B, a novel thyroid-specific isozyme of 3',5'-cyclic nucleotide phosphodiesterase. Biochem Biophys Res Commun 250:751-6 (1998). PubMed PMID: 9784418
Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991). PubMed PMID: 1832709

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDE8A (N-term) antibody

Anti-PDE8A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €345.00
  OriGene Technologies, Inc.

PDE8A antibody

WB Suggested Anti-PDE8A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE8A antibody

WB Suggested Anti-PDE8A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDE8A control peptide

PDE8A
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE8A (N-term) antibody

Western blot usingAP10250PU-N 1:500 diluted. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE8A control peptide

PDE8A
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE8A antibody

PDE8A Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE8A control peptide

PDE8A
0.25 mg / €250.00
  Acris Antibodies GmbH

PDE8A (C-term) antibody

PDE8A Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

PDE8A (Center) antibody

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PDE8A antibody (Center) (AP11453PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDE8A antibody

Western Blot analysis of PDE8A expression in transfected 293T cell line (H00005151-T01) by PDE8A MaxPab polyclonal antibody.

Lane 1: PDE8A transfected lysate(91.19 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PDE8A antibody

Western Blot analysis of PDE8A expression in transfected 293T cell line by PDE8A monoclonal antibody (M02), clone 1H6.

Lane 1: PDE8A transfected lysate(93.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1H6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDE8A antibody

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PDE8A polyclonal antibody ( Cat # PAB1878 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PDE8A (C-term) antibody

PDE8A Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PDE8A (C-term) antibody

PDE8A Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDE8A

PDE8A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PDE8A

PDE8A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

PDE8A

PDE8A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PDE8A

PDE8A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PDE8A 293T Cell Transient Overexpression Lysate(Denatured)

PDE8A 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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