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PDF antibody

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Principal name

PDF antibody

Alternative names for PDF antibody

PDF1A, Polypeptide deformylase, Peptide deformylase mitochondrial

SwissProt ID

Q9HBH1 (Human)

Gene ID

64146 (PDF)

Ncbi ID

9606, NP_071736.1

Available reactivities

Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PDF antibody

Protein synthesis proceeds after formylation of methionine by methionyl-tR formyl transferase (FMT) and transfer of the charged initiator f-met tR to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of scent peptides. In eubacteria, deformylation of scent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a tural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq].

H00064146-B01P - PDF
PDF MaxPab polyclonal antibody. Western Blot analysis of PDF expression in human spleen.
H00064146-B01P - PDF
Western Blot analysis of PDF expression in transfected 293T cell line (H00064146-T02) by PDF MaxPab polyclonal antibody.Lane 1: PDF transfected lysate(26.73 KDa).Lane 2: Non-transfected lysate.
TA502988 - PDF
Anti-PDF mouse monoclonal antibody (TA502988) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF(RC205788 ).
TA502988 - PDF
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (RC205788, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.
TA502988 - PDF
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PDF monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
TA502988 - PDF
Flow cytometric Analysis of Hela cells, using anti-PDF antibody(TA502988),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
TA502988 - PDF
HEK293T cells transfected with either RC205788 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDF antibody(TA502988), and then analyzed by flow cytometry.
TA502988 - PDF
Flow cytometric Analysis of Jurkat cells, using anti-PDF antibody(TA502988),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
TA502989 - PDF
Anti-PDF mouse monoclonal antibody (TA502989) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF(RC205788 ).
TA502989 - PDF
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (RC205788, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.
TA502989 - PDF
HEK293T cells transfected with either RC205788 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDF antibody(TA502989), and then analyzed by flow cytometry.
TA502989 - PDF
Flow cytometric Analysis of Hela cells, using anti-PDF antibody(TA502989),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
  1. Quick-Filters:
  2. Highly Validated (2)

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDF antibody
  PDF mouse monoclonal antibody, clone OTI3F3 (formerly 3F3)  
Anti-PDF mouse monoclonal antibody (TA502988) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF(RC205788 ). Mouse IgG2b OTI3F3 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+5 additional images

Highly validated antibody!

TrueMAB

PDF antibody
  PDF mouse monoclonal antibody, clone OTI3C12 (formerly 3C12)  
Anti-PDF mouse monoclonal antibody (TA502989) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF(RC205788 ). Mouse IgG1 OTI3C12 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

TrueMAB

PDF antibody
PDF Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PDF - human gene knockout kit via CRISPR
PDF - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PDF - human gene knockout kit via CRISPR
PDF - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDF - human gene knockout kit via CRISPR
PDF - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDF - human gene knockout kit via CRISPR
PDF - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

7 Item(s)

per page

Available Filters

Product Type
  1. CRISPR (4)
  2. Primary Antibody (3)
Clonality
  1. Monoclonal (2)
  2. Polyclonal (1)
Target
  1. PDF (3)
Reactivity
  1. Human (Hu) (3)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Flow cytometry (F) (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  4. Frozen sections (C) (1)
  5. Enzyme Immunoassay (E) (1)
Presentation
  1. Purified (2)
  2. Serum (1)
Host
  1. Mouse (2)
  2. Rabbit (1)
Images
  1. 1 image (2)
  2. 3+ images (2)
  3. 5+ images (1)
Images: Applications
  1. Flow cytometry (F) (2)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  3. Western blot / Immunoblot (WB) (2)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. Acris - OriGene (7)
Service
  • OriGene EU
  • Acris Antibodies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info@acris-online.de
  • orders: ordering@acris-online.de
 
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