PDGFA antibody

Principal name

PDGFA antibody

Alternative names for PDGFA antibody

Platelet-derived growth factor subunit A, PDGF alpha, PDGF A, PDGF-A, Platelet-derived growth factor alpha, PDGF subunit A, Platelet-derived growth factor A chain, PDGF1, PDGF-1

SwissProt ID

P04085 (Human), P13698 (Xenla), P20033 (Mouse), P28576 (Rat), P34007 (Rabit), Q2KJ15 (Bovin)

Gene ID

5154 (PDGFA), 25266 (Pdgfa), 18590

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Rb (Rabbit), Can (Canine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PDGFA antibody

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5 The standard product used in this kit is recombinant rat PDGF-AB(A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDGFA antibody

  Anti-PDGFA Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PDGFA antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

  Anti-PDGFA Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human lung and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PDGFA antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

  Biotinylated Anti-Human PDGF-AA Rabbit Polyclonal Antibody  
To detect hPDGF-AA by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

  Anti-Human PDGF-AA Rabbit Polyclonal Antibody  
To detect hPDGF-AA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-AA (TA328414) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

  Rabbit Polyclonal Anti-PDGFA Antibody  
Host: Rabbit; Target Name: PDGFA; Sample Tissue: U937 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

  Rabbit Polyclonal Anti-PDGFA Antibody  
Western blot analysis of PDGFA expression in Jurkat cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

Immunohistochemistry analyzes of PDGF-A antibody (Cat.-No.: AP20635PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PDGFA (PDGF-AA) antibody

Direct ELISA using PDGFA Antibody - Biotin Cat.-No PP1061B Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

PDGFA (PDGF-AA) antibody

Direct ELISA using PDGFA Antibody - Biotin Cat.-No PP1061B Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

PDGFA (PDGF-AA) antibody

Immunohistochemistry: This PDGFA antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 µg/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

PDGFA (PDGF-AA) antibody

Immunohistochemistry: This PDGFA antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PDGFA - human gene knockout kit via CRISPR

PDGFA - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PDGFA - human gene knockout kit via CRISPR

PDGFA - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDGFA - human gene knockout kit via CRISPR

PDGFA - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDGFA - human gene knockout kit via CRISPR

PDGFA - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdgfa - mouse gene knockout kit via CRISPR

Pdgfa - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pdgfa - mouse gene knockout kit via CRISPR

Pdgfa - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdgfa - mouse gene knockout kit via CRISPR

Pdgfa - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdgfa - mouse gene knockout kit via CRISPR

Pdgfa - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDGFA (transcript variant 2)

  Purified recombinant protein of Homo sapiens platelet-derived growth factor alpha polypeptide (PDGFA), transcript variant 2  
PDGFA Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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