PDGFA antibody

Principal name

PDGFA antibody

Alternative names for PDGFA antibody

Platelet-derived growth factor subunit A, PDGF alpha, PDGF A, PDGF-A, Platelet-derived growth factor alpha, PDGF subunit A, Platelet-derived growth factor A chain, PDGF1, PDGF-1

SwissProt ID

P04085 (Human), P13698 (Xenla), P20033 (Mouse), P28576 (Rat), P34007 (Rabit), Q2KJ15 (Bovin)

Gene ID

5154 (PDGFA), 25266 (Pdgfa), 18590, 24628 (Pdgfb)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Can (Canine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Functional assay (FN), Frozen Sections (C)

Background of PDGFA antibody

The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5 The standard product used in this kit is recombinant rat PDGF-AB(A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.

Items 1 to 20 of 65 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDGFA antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PDGFA antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

Predicted cell location: Cytoplasm. Positive control: Human lung and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PDGFA antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

To detect hPDGF-AA by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

To detect hPDGF-AA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-AA (TA328414) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

Host: Rabbit; Target Name: PDGFA; Sample Tissue: U937 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

Western blot analysis of PDGFA expression in Jurkat cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFA antibody

Immunohistochemistry analyzes of PDGF-A antibody (Cat.-No.: AP20635PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PDGFA (PDGF-AA) antibody

Direct ELISA using PDGFA Antibody - Biotin Cat.-No PP1061B Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

PDGFA (PDGF-AA) antibody

Direct ELISA using PDGFA Antibody - Biotin Cat.-No PP1061B Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

PDGFA (PDGF-AA) antibody

Immunohistochemistry: This PDGFA antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 µg/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

PDGFA (PDGF-AA) antibody

Immunohistochemistry: This PDGFA antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PDGFA antibody

PDGFA Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PDGFA antibody

Western Blot analysis of PDGFA expression in transfected 293T cell line (H00005154-T01) by PDGFA MaxPab polyclonal antibody.

Lane 1: PDGFA transfected lysate(23.21 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PDGFA antibody

Immunoprecipitation of PDGFA transfected lysate using anti-PDGFA MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PDGFA MaxPab mouse polyclonal antibody (B01) (H00005154-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

PDGFA antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PDGFA and PLCG1. Huh7 cells were stained with anti-PDGFA rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PDGFA antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with PDGFA polyclonal antibody ( Cat # PAB3678 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PDGFA antibody

PDGFA Sheep Hu E
1 ml / €560.00
  Abnova Taiwan Corp.

PDGFA antibody

Immunohistochemical analysis of PDGFA staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rb, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

PDGFA antibody

Immunohistochemical analysis of PDGFA staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rb, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

PDGFA antibody

Immunohistochemical analysis of PDGFA staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rb, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Items 1 to 20 of 65 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn