PDGFB antibody

Principal name

PDGFB antibody

Alternative names for PDGFB antibody

Platelet-derived growth factor subunit B, PDGF beta, PDGF B, PDGF-B, Platelet-derived growth factor beta, PDGF subunit B, Platelet-derived growth factor B chain, PDGF2, PDGF-2, c-Sis, SIS, Becaplermin

SwissProt ID

P01127 (Human), P12919 (Felca), P20034 (Pig), P31240 (Mouse), Q05028 (Rat), Q6Q7I7 (Canfa), Q95229 (Sheep)

Gene ID

5155 (PDGFB), 18591 (Pdgfb)

Available reactivities

Hu (Human), Rt (Rat), Hst (Hamster), Ms (Mouse), Sh (Sheep), Bov (Bovine), Can (Canine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat, Sheep

Available applications

Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), ELISA (detection) (E(detection))

Background of PDGFB antibody

The platelet-derived growth factor (PDGF) family consists of four different polypeptide chains; PDGF-A, PDGF-B, PDGF-C and PDGF-D, sharing approximately 25% sequence homology. The biological effects of PDGF-B are exerted via two receptor tyrosine kinases, PDGFRA and PDGFRB. PDGF-B is expressed predominantly in platelets and the heart, but has been detected in the majority of human organs. PDGF-B is a potent mitogen for mesenchymal cells such as fibroblasts, smooth muscle cells, and neuroectodermal cells such as oligodendrocytes, and plays a critical role in wound healing and tumor development. PDGF-B is an important growth factor contributing to angiogenesis and possibly lymphatic metastasis due to lymphangiogenesis. Additionally, PDGF-B plays a crucial role in wound healing. Studies have also suggested that PDGF-B is involved in the CNS including having a neuroprotective or neurotrophic effect in the developing or adult CNS, promoting angiogenic responses to injury in the CNS and remyelination. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Van Steensel L., et al. (2012) J Clin Endocrinol Metab. 97(6): 37062-37071.Malabanan KP, et al. (2012) Am J Pathol. 180(6): 2590-7.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDGFB (222-233) antibody

Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Fe, Hu, Mky, Ms, Op, Prim, Rt, Sh P
50 µg / €470.00
  Acris Antibodies GmbH

PDGFB (101-116) antibody

Figure 1. Formalin-fixed, paraffin-embedded human angiosarcoma stained with PDGF antibody (Cat. DP145) using peroxidase-conjugate and AEC. Note cytoplasmic staining of tumor cells. Rabbit Purified Hu, Rt C, P
1 ml / €580.00
  Acris Antibodies GmbH

PDGFB (101-116) antibody

Figure 1. Formalin-fixed, paraffin-embedded human angiosarcoma stained with PDGF antibody (Cat. DP145) using peroxidase-conjugate and AEC. Note cytoplasmic staining of tumor cells. Rabbit Purified Hu, Rt C, P
0.5 ml / €380.00
  Acris Antibodies GmbH

PDGFB antibody

Western blot analysis of extracts from NIH/3T3 cells, using PDGFB antibody (#TA314326).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

Western blot analysis using recombinant human PDGF-BB. Rabbit Purified Hu WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

Western blot analysis using recombinant murine PDGF-BB. Lane 1: 50 ng, Lane 2: 250 ng, Lane 3: 1 ug. Rabbit Purified Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

Western blot analysis of PDGF-B in rat liver tissue lysate with PDGF-B antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

Predicted cell location: Cytoplasm. Positive control: Human liver and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using PDGFB antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

Western blot analysis on NIH-3T3 cell lysate using PDGFB Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

Western blot analysis of extracts of A431 cell lines using PDGFB antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

To detect hPDGF-BB by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

To detect hPDGF-BB by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (TA328416) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

WB Suggested Anti-PDGFB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: 293T cell lysateThere is BioGPS gene expression data showing that PDGFB is expressed in HEK293T Rabbit IgG Purified Bov, Can, Hu, Ms, Rb, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDGFB antibody

ELISA: PDGFB Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDGFB (PDGF-BB / - AB) antibody

Staining of normal formalin fixed, paraffin embedded human skin with Rabbit anti Human PDGF BB Cat.-No AP05490PU-N) Rabbit IgG Purified Hu E, FN, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

PDGFB antibody

 Immunohistochemistry (IHC) analysis of PDGFB antibody (Cat.-No.: AP06282PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PDGFB (PDGF-BB) antibody

PDGFB Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

PDGFB (PDGF-BB) antibody

PDGFB Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

PDGFB (PDGF-BB) antibody

Formalin-Fixed, Paraffin-Embedded Sections of normal Human skin stained with PDGF-BB antibody Cat.-No PP1062P Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

PDGFB (PDGF-BB) antibody

Formalin-Fixed, Paraffin-Embedded Sections of normal Human skin stained with PDGF-BB antibody Cat.-No PP1062P Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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