PDHA1 antibody

Principal name

PDHA1 antibody

Alternative names for PDHA1 antibody

PHE1A, PDHE1-A type I, Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial

SwissProt ID

A7MB35 (Bovin), P08559 (Human), P16387 (Yeast), P26284 (Rat), P29804 (Pig), P35486 (Mouse), P49823 (Canfa), P52899 (Caeel), Q10489 (Schpo)

Gene ID

5160 (PDHA1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F)

Background of PDHA1 antibody

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT); and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Glushakova, L.G., et al. Mol. Genet. Metab. 98(3):289-299(2009)
Joao Silva, M., et al. Eur. J. Pediatr. 168(1):17-22(2009)
Boichard, A., et al. Mol. Genet. Metab. 93(3):323-330(2008)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDHA1 antibody

Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. TA308687 diluted at 1:5000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

Anti-PDHA1 mouse monoclonal antibody (TA502696) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1(RC201831). Mouse IgG2b OTI2C10 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDHA1 antibody

Anti-PDHA1 mouse monoclonal antibody (TA502704) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1(RC201831). Mouse IgG2b OTI2B10 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PDHA1 antibody

Immunoperoxidase of purified MaxPab antibody to PDHA1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml] Mouse Purified Hu E(detection), P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PDHA1 antibody

Western blot analysis of extracts from A549, HepG2 and Jurkat cells, using PDHA1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

Predicted band size: 43 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

Western blot analysis of extracts of various cell lines, using PDHA1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [TA336570] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretin Rabbit Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €379.00
  OriGene Technologies, Inc.

PDHA1 antibody

Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293,1299,mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa. Mouse IgG1 3H2-F8-B5 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

Western blot analysis of PDHA1 (arrow) using PDHA1 Antibody Cat.-No AP53240PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDHA1 (Center) antibody

Western blot analysis of PDHA1 Antibody (Center) Cat.-No AP53241PU-N in Hela cell line lysates (35ug/lane). PDHA1 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDHA1 antibody

PDHA1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PDHA1 pSer293 antibody

Western blot analysis of PDHA1 in an in vitro autophosphorylation of PDH complex in response to different stimulants with PDHA1 (phospho S293) polyclonal antibody ( Cat # PAB12079 ).
Such stimulants are : ( - ) none, H2O2-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator.
Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center. Rabbit Hu WB
50 µl / €560.00
  Abnova Taiwan Corp.

PDHA1 antibody

mouse brain extract; WB Suggested Anti-PDHA1 Antibody . . Positive Control: Lane 1: 80ug mouse brain extract . Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience. Secondry Antibody Dilution : 1:20,000. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science; PDHA1 antibody - N-terminal region (ARP48136_T100) in mouse brain extract cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

PDHA1 antibody

human NT2; WB Suggested Anti-PDHA1 Antibody . . Positive Control: Lane 1: 60ug human NT2 cell line . Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience. Secondry Antibody Dilution : 1:20,000. Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science; PDHA1 antibody - C-terminal region (ARP48137_T100) in human NT2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

PDHA1 antibody

PDHA1 Rabbit Biotin Hu WB
50 µl / €260.00
  Novus Biologicals Inc.

PDHA1 antibody

PDHA1 Rabbit DyLight488 Hu WB
50 µl / €270.00
  Novus Biologicals Inc.

PDHA1 antibody

PDHA1 Rabbit HRP Hu WB
50 µl / €270.00
  Novus Biologicals Inc.

PDHA1 antibody

PDHA1 Rabbit Aff - Purified Hu ICC/IF, WB
25 µl / €200.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDHA1

PDHA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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