PDHA1 antibody

Principal name

PDHA1 antibody

Alternative names for PDHA1 antibody

PHE1A, PDHE1-A type I, Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial

SwissProt ID

A7MB35 (Bovin), P08559 (Human), P16387 (Yeast), P26284 (Rat), P29804 (Pig), P35486 (Mouse), P49823 (Canfa), P52899 (Caeel), Q10489 (Schpo)

Gene ID

5160 (PDHA1), 18597 (Pdha1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of PDHA1 antibody

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT); and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex.The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Glushakova, L.G., et al. Mol. Genet. Metab. 98(3):289-299(2009)
Joao Silva, M., et al. Eur. J. Pediatr. 168(1):17-22(2009)
Boichard, A., et al. Mol. Genet. Metab. 93(3):323-330(2008)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDHA1 antibody

  Rabbit Polyclonal antibody to Pyruvate Dehydrogenase E1 alpha (pyruvate dehydrogenase (lipoamide) alpha 1)  
Sample (20 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. TA308687 diluted at 1:5000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

  PDHA1 mouse monoclonal antibody, clone OTI2C10 (formerly 2C10)  
Anti-PDHA1 mouse monoclonal antibody (TA502696) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1(RC201831). Mouse IgG2b OTI2C10 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDHA1 antibody

  PDHA1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10)  
Anti-PDHA1 mouse monoclonal antibody (TA502704) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1(RC201831). Mouse IgG2b OTI2B10 Purified Hu F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PDHA1 antibody

  Rabbit polyclonal anti-PDHA1 antibody  
Western blot analysis of extracts from A549, HepG2 and Jurkat cells, using PDHA1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

  Anti-PDHA1 Rabbit Polyclonal Antibody  
Predicted band size: 43 kDa . Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

  Rabbit anti-PDHA1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using PDHA1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

  Rabbit Polyclonal Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody  
Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [TA336570] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretin Rabbit Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €379.00
  OriGene Technologies, Inc.

PDHA1 antibody

  Rabbit Polyclonal Anti-PDHA1 Antibody  
WB Suggested Anti-PDHA1 Antibody; Positive Control: Lane 1: 80ug mouse brain extract; Primary Antibody Dilution: 1: 500; Secondary Antibody: IRDye 800 CW goat anti-rabbit from Li-COR Bioscience; Secondry Antibody Dilution: 1: 20, 000; Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

  Rabbit Polyclonal Anti-PDHA1 Antibody  
WB Suggested Anti-PDHA1 Antibody; Positive Control: Lane 1: 60ug human NT2 cell line; Primary Antibody Dilution: 1: 500; Secondary Antibody: IRDye 800 CW goat anti-rabbit from Li-COR Bioscience; Secondry Antibody Dilution: 1: 20, 000; Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

  Mouse Monoclonal pyruvate dehydrogenase (lipoamide) alpha 1 Antibody  
Western blot detection of pyruvate dehydrogenase (lipoamide) alpha 1 in 293,1299,mEsc and Tc1 cell lysates using pyruvate dehydrogenase (lipoamide) alpha 1 mouse mAb (1:1000 diluted).Predicted band size: 43KDa.Observed band size: 43KDa. Mouse IgG1 3H2-F8-B5 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDHA1 antibody

Western blot analysis of PDHA1 (arrow) using PDHA1 Antibody Cat.-No AP53240PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDHA1 gene. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDHA1 (Center) antibody

Western blot analysis of PDHA1 Antibody (Center) Cat.-No AP53241PU-N in Hela cell line lysates (35ug/lane). PDHA1 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PDHA1 - human gene knockout kit via CRISPR

PDHA1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PDHA1 - human gene knockout kit via CRISPR

PDHA1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDHA1 - human gene knockout kit via CRISPR

PDHA1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDHA1 - human gene knockout kit via CRISPR

PDHA1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdha1 - mouse gene knockout kit via CRISPR

Pdha1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pdha1 - mouse gene knockout kit via CRISPR

Pdha1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdha1 - mouse gene knockout kit via CRISPR

Pdha1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdha1 - mouse gene knockout kit via CRISPR

Pdha1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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