PDI / P4HB antibody

Principal name

PDI / P4HB antibody

Alternative names for PDI / P4HB antibody

ERBA2L, PDIA1, PO4DB, Protein disulfide-isomerase, ER marker, Prolyl 4-hydroxylase subunit beta p55, Cellular thyroid hormone-binding protein

SwissProt ID

P04785 (Rat), P05307 (Bovin), P07237 (Human), P09102 (Chick), P09103 (Mouse), P21195 (Rabit), Q8R4U2 (Crigr)

Gene ID

25506 (P4hb), 5034 (P4HB)

Available reactivities

Hst (Hamster), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Xen (Xenopus), Bov (Bovine), Can (Canine), GP (Guinea Pig), Mky (Monkey), Sh (Sheep), Chk (Chicken), Rb (Rabbit), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))

Background of PDI / P4HB antibody

P4HB is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

General / background references:Basu S & Srivastava PK Calreticulin, a peptide-binding chaperone of the endoplasmic reticulum, elicits tumor- and peptide-specific immunity. J Exp Med 189:797-802 (1999). PubMed PMID: 10049943
Kuznetsov G et al. Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J Biol Chem 269:22990-5 (1994). PubMed PMID: 7916014
Kaetzel CS et al. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J 241:39-47 (1987). PubMed PMID: 3566712

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

P4HB antibody

AP08767PU-N P4HB antibody staining of Formalin-Fixed, Paraffin-Embedded Human small intestine. Rabbit IgG Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PDI / P4HB antibody

Sample (20 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308403 diluted at 1:10000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDI / P4HB antibody

Immunofluorescence of purified MaxPab antibody to P4HB on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PDI / P4HB antibody

Predicted band size: 57 kDa . Positive control: THP-1, SW620, 6T-CEM, NIH3T3 and RAW264.7cell; liver and . kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1:THP-1 cell lysate Lane2: SW620 cell lysate Lane13:6T-CEM cell lysate Lane4:NIH3T3 cell lysate Lane5: RAW264.7cell lysate Lane6:Liver tissue lysate Lane7:Kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDI / P4HB antibody

Western blot analysis of extracts of various cell lines, using P4HB antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDI / P4HB antibody

Western blot analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDI / P4HB (C-term) antibody

AP17615PU-N P4HB antibody staining of Formalin-Fixed and paraffin-Embedded Human hepatocarcinoma using peroxidase-conjugated to the secondary antibody, followed by DAB staining. Clinical relevance has not been evaluated. Rabbit Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDI / P4HB antibody

PDI / P4HB Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PDI / P4HB antibody

PDI / P4HB Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PDI / P4HB antibody

Human kidney; P4HB antibody - N-terminal region (ARP48150_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rb, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

PDI / P4HB antibody

Human HepG2; WB Suggested Anti-P4HB Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. P4HB is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; P4HB antibody - C-terminal region (ARP48151_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PDI / P4HB antibody

PDI / P4HB Mouse IgG2b RL77 Ascites Hst, Hu, Ms, Por, Rt, Xen F, ICC/IF, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

PDI / P4HB antibody

PDI / P4HB Rabbit IgG Liquid Hu, Mky, Ms, Rt ICC/IF, P, WB
50 µl / €480.00
  Novus Biologicals Inc.

PDI / P4HB antibody

Western Blot: P4HB Antibody [NBP1-57949] - This Anti-P4HB antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.5ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

PDI / P4HB antibody

PDI / P4HB Mouse Aff - Purified Hu WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PDI / P4HB antibody

Immunohistochemistry-Paraffin: P4HB Antibody [NBP1-84051] - Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDI / P4HB

PDI / P4HB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

PDI / P4HB (18-508, His-tag)

Recombinant human P4HB, 18-508aa, His-tagged Human Purified > 90 % by SDS-PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

PDI / P4HB (18-508, His-tag)

Recombinant human P4HB, 18-508aa, His-tagged Human Purified > 90 % by SDS-PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

PDI / P4HB (20-509, His-tag)

PDI / P4HB Mouse Purified > 95 % by SDS – PAGE Insect cells
0.25 mg / €940.00
  Acris Antibodies GmbH

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