PDIA3 antibody
Principal name
PDIA3 antibody
Alternative names for PDIA3 antibody
Protein disulfide-isomerase A3, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, ER marker, Disulfide isomerase ER-60
Ncbi ID
9606, NP_005304, NP_005304.3, NP_031978.2, NM_007952, NM_017319, NM_005313
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PDIA3 antibody
ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
General readings
1. Koivunen P, Horelli-Kuitunen N, Helaakoski T, Karvonen P, Jaakkola M, Palotie A, Kivirikko KI.
Structures of the human gene for the protein disulfide isomerase-related polypeptide ERp60 and a processed gene and assignment of these genes to 15q15 and 1q21.
Genomics. 1997 Jun 15;42(3):397-404.
PMID: 9205111
PDIA3 polyclonal antibody ( Cat # PAB6295 ) staining ( 1 µg/ml ) of HepG2 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
PDIA3 MaxPab polyclonal antibody. Western Blot analysis of PDIA3 expression in human stomach.
Western Blot analysis of PDIA3 expression in transfected 293T cell line (H00002923-T01) by PDIA3 MaxPab polyclonal antibody.Lane 1: PDIA3 transfected lysate(55.55 KDa).Lane 2: Non-transfected lysate.
PDIA3 MaxPab polyclonal antibody. Western Blot analysis of PDIA3 expression in A-431.
PDIA3 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDIA3 expression in mouse liver.
PDIA3 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDIA3 expression in human kidney.
Western Blot analysis of PDIA3 expression in transfected 293T cell line (H00002923-T02) by PDIA3 MaxPab polyclonal antibody.Lane 1: PDIA3 transfected lysate(56.80 KDa).Lane 2: Non-transfected lysate.
PDIA3 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDIA3 expression in HeLa.
Recombinant human PDIA3, 25-505 aa, His-tagged: 15% SDS-PAGE (3 µg)
(LEFT) Western blot analysis of PDIA3 Antibody (Center) (AP17626PU-N) in A2058, A375, Ramos cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml) (LEFT) Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center) (AP17626PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .
(LEFT) Western blot analysis of PDIA3 Antibody (C-term) (AP17627PU-N) in A2058 cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (C-term) (AP17627PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .
Colon, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot Analysis of ERp57 (Grp58): Lane 1: MWMLane 2: CHO-K1 lysateLane 3: Human Liver MicrosomesLane 4: Rat Liver MicrosomesLane 5: Mouse Liver Microsomes
Western blot analysis of canine MDCK lysate.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA3 (RC205940, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA3.
Anti-PDIA3 mouse monoclonal antibody (TA504993) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDIA3(RC205940).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA3 (RC205940, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA3.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA3 (RC205940, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA3.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA3 (RC205940, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA3.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA3 (RC205940, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA3.
TA302680 staining (1µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of extracts of various cell lines using PDIA3 antibody.
Immunohistochemistry of paraffin-embedded rat kidney using PDIA3 antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of HeLa cell using PDIA3 antibody. Blue: DAPI for nuclear staining.
- Quick-Filters:
- Highly Validated (4)
- Overnight Delivery (6)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA504995 | PDIA3 antibody |
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| PDIA3 (ERp57) mouse monoclonal antibody, clone OTI3E1 (formerly 3E1) | |||||||
 |
Mouse | IgG2b | OTI3E1 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +10 additional images |
Highly validated antibody!
|
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| TA505008 | PDIA3 antibody |
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| PDIA3 (ERp57) mouse monoclonal antibody, clone OTI4D7 (formerly 4D7) | |||||||
 |
Mouse | IgG2a | OTI4D7 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA505010 | PDIA3 antibody |
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| PDIA3 (ERp57) mouse monoclonal antibody, clone OTI3H5 (formerly 3H5) | |||||||
 |
Mouse | IgG2a | OTI3H5 | Purified | Hu | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| AP22891PU-N | PDIA3 antibody |
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 |
Rabbit | Purified | Bov, Can, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt | P, WB |
50 µg /
€470.00
|
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| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AB0004-20 | PDIA3 (C-term) antibody |
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 |
Goat | IgG | Aff - Purified | Can, Hu, Mky, Ms, Rt | C, ICC/IF, P, WB |
20 µl /
€50.00
|
|
| SICGEN | |||||||
| +1 additional image |
Acris recommends this product for Western blotting. |
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| AB0004-200 | PDIA3 (C-term) antibody |
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|
Goat | IgG | Aff - Purified | Can, Hu, Mky, Ms, Rt | C, ICC/IF, P, WB |
0.6 mg /
€300.00
|
|
| SICGEN | |||||||
| +1 additional image |
Acris recommends this product for Western blotting. |
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| TA302680 | PDIA3 (C-term) antibody |
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| Goat Polyclonal Antibody against PDIA3 / ERp57 / GRP58 | |||||||
 |
Goat | Aff - Purified | Bov, Hu, Ms, Rt | E, WB |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA309330 | PDIA3 antibody |
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| Mouse Monoclonal anti-PDIA3 Antibody | |||||||
 |
Mouse | IgG1 | MaP.ERP57 | Purified | Bov, Can, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt | C, IP, P, WB |
50 µg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| TA326353 | PDIA3 antibody |
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| Mouse monoclonal Erp57 (Grp58) Antibody | |||||||
|
Mouse | IgG1 | MaP.Erp57 | Purified | Bov, Can, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt | C, IP, P, WB |
0.2 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| TA326918 | PDIA3 antibody |
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| Rabbit anti-PDIA3 PPolyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA504990 | PDIA3 antibody |
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| PDIA3 (ERp57) mouse monoclonal antibody, clone OTI3D2 (formerly 3D2) | |||||||
 |
Mouse | IgG1 | OTI3D2 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA504993 | PDIA3 antibody |
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| PDIA3 (ERp57) mouse monoclonal antibody, clone OTI2D2 (formerly 2D2) | |||||||
 |
Mouse | IgG1 | OTI2D2 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
||||||
| AB0003-20 | PDIA3 (C-term) antibody |
||||||
 |
Goat | IgG | Aff - Purified | Can, Hu, Mky, Ms, Rt | C, ICC/IF, P, WB |
20 µl /
€50.00
|
|
| SICGEN | |||||||
| AB0003-200 | PDIA3 (C-term) antibody |
||||||
|
Goat | IgG | Aff - Purified | Can, Hu, Mky, Ms, Rt | C, ICC/IF, P, WB |
0.4 mg /
€300.00
|
|
| SICGEN | |||||||
| AB0003-500 | PDIA3 (C-term) antibody |
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 |
Goat | IgG | Aff - Purified | Can, Hu, Mky, Ms, Rt | C, ICC/IF, P, WB |
1.0 mg /
€450.00
|
|
| SICGEN | |||||||
| AB0004-500 | PDIA3 (C-term) antibody |
||||||
 |
Goat | IgG | Aff - Purified | Can, Hu, Mky, Ms, Rt | C, ICC/IF, P, WB |
1.5 mg /
€450.00
|
|
| SICGEN | |||||||
| AP17626PU-N | PDIA3 (Center) antibody |
||||||
 |
Rabbit | Purified | Hu | WB |
0.4 ml /
€370.00
|
||
| Acris Antibodies GmbH | |||||||
| AP17627PU-N | PDIA3 (C-term) antibody |
||||||
 |
Rabbit | Hu | WB |
0.4 ml /
€370.00
|
|||
| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205940 | PDIA3 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN205940BN | PDIA3 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
