PDIA3 antibody

Principal name

PDIA3 antibody

Alternative names for PDIA3 antibody

Protein disulfide-isomerase A3, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, ER marker, Disulfide isomerase ER-60

SwissProt ID

P11598 (Rat), P27773 (Mouse), P30101 (Human), P38657 (Bovin)

Gene ID

2923 (PDIA3)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Hst (Hamster), Mky (Monkey), Por (Porcine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of PDIA3 antibody

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

General readings

1. Koivunen P, Horelli-Kuitunen N, Helaakoski T, Karvonen P, Jaakkola M, Palotie A, Kivirikko KI.
Structures of the human gene for the protein disulfide isomerase-related polypeptide ERp60 and a processed gene and assignment of these genes to 15q15 and 1q21.
Genomics. 1997 Jun 15;42(3):397-404.
PMID: 9205111

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDIA3 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA504995) Mouse IgG2b OTI3E1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDIA3 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505008) Mouse IgG2a OTI4D7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDIA3 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505010) Mouse IgG2a OTI3H5 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PDIA3 antibody

Colon, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PDIA3 antibody

PDIA3 MaxPab rabbit polyclonal antibody. Western Blot analysis of PDIA3 expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PDIA3 (C-term) antibody

PDIA3 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / €50.00
  SICGEN

PDIA3 (C-term) antibody

PDIA3 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.6 mg / €300.00
  SICGEN

PDIA3 (C-term) antibody

TA302680 staining (1µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

PDIA3 (C-term) antibody

TA302680 staining (1µg/ml) of HepG2 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDIA3 antibody

Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of the antibody Mouse IgG1 MaP.ERP57 Purified Bov, Can, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt C, IP, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDIA3 antibody

Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of the antibody Mouse IgG1 MaP.Erp57 Purified Bov, Can, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt C, IP, P, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

PDIA3 antibody

Western blot analysis of extracts of various cell lines using PDIA3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDIA3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA3 (RC205940, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA3. Mouse IgG1 OTI3D2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDIA3 antibody

Anti-PDIA3 mouse monoclonal antibody (TA504993) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDIA3(RC205940). Mouse IgG1 OTI2D2 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PDIA3 (C-term) antibody

PDIA3 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / €50.00
  SICGEN

PDIA3 (C-term) antibody

PDIA3 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.4 mg / €300.00
  SICGEN

PDIA3 (C-term) antibody

PDIA3 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
1.0 mg / €450.00
  SICGEN

PDIA3 (C-term) antibody

PDIA3 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
1.5 mg / €450.00
  SICGEN

PDIA3 (Center) antibody

(LEFT) Western blot analysis of PDIA3 Antibody (Center) (AP17626PU-N) in A2058, A375, Ramos cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml) (LEFT) Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center) (AP17626PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) . Rabbit Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDIA3 (C-term) antibody

(LEFT) Western blot analysis of PDIA3 Antibody (C-term) (AP17627PU-N) in A2058 cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (C-term) (AP17627PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) . Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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