PDK1 antibody

Principal name

PDK1 antibody

Alternative names for PDK1 antibody

Pyruvate dehydrogenase kinase isoform 1, Pyruvate dehydrogenase [lipoamide] kinase isozyme 1 mitochondrial

SwissProt ID

Q15118 (Human), Q63065 (Rat), Q8BFP9 (Mouse)

Gene ID

5163 (PDK1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Bov (Bovine), African clawed frog, Mky (Monkey), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of PDK1 antibody

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005 Feb 18;307(5712):1098-101.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDK1 antibody

Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 antibody Cat.-No AM06566SU-N with DAB staining. Mouse IgG1 4A11 Ascites Hu, Mky, Rt E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

PDK1 antibody

  Rabbit Polyclonal Antibody against PDK1  
Detection of PDK1 in human heart lysate at 2 ug/ml Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK1 antibody

  Rabbit Polyclonal Antibody against PDK1  
Detection of PDK1 in human heart lysate at 0.5 ug/ml. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK1 antibody

  Goat Polyclonal Antibody against PDK1  
TA303069 (1µg/ml) staining of Rat Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDK1 antibody

  Rabbit polyclonal PDK1 antibody  
Western blot analysis of extracts from Hela cells, using PDK1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK1 antibody

  Rabbit Polyclonal anti-PDK1 antibody  
WB Suggested Anti-PDK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDK1 (29-436) antibody

ICC/IF analysis of PDK1 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human PDK1 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG1 AT2D6 Purified Hu, Rt E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PDK1 (29-436) antibody

ICC/IF analysis of PDK1 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human PDK1 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green). Mouse IgG1 AT2D6 Purified Hu, Rt E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

PDK1 (N-term) antibody

The anti-PDK1 Pab is used in Western blot to detect PDK1 in mouse kidney tissue lysate. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDK1 antibody

Skeletal muscle. Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Can, Hu, Mky, Ms, Por, Rb, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDK1

  Recombinant protein of human pyruvate dehydrogenase kinase, isozyme 1 (PDK1), nuclear gene encoding mitochondrial protein  
PDK1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PDK1 (29-436, His-tag)

PDK1 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

PDK1 (29-436, His-tag)

PDK1 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

Lysates (Knockout)

Catalog No.    

PDK1-knockout Cell lysate

PDK1-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

14 Item(s)

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