PDK4 antibody

Principal name

PDK4 antibody

Alternative names for PDK4 antibody

Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase lipoamide kinase isozyme 4 mitochondrial

SwissProt ID

O54937 (Rat), O70571 (Mouse), Q16654 (Human)

Gene ID

5166 (PDK4), 27273 (Pdk4), 89813

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PDK4 antibody

PDK4 (pyruvate dehydrogese kise isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptiol initiation complex responsible for PDK4 activation. Insulin promotes ictivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogese complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.

General readings

Kimura,K., (2006) Genome Res. 16 (1), 55-65

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDK4 (C-term) antibody

  Rabbit Polyclonal Antibody against PDK4 (C-term)  
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (Cat. #TA302046), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDK4 (C-term) antibody

Human Adrenal (formalin-fixed, paraffin-embedded) stained with PDK4 antibody AP22786PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms E, ICC/IF, P, WB
0.2 ml / €470.00
  Acris Antibodies GmbH

PDK4 antibody

  Anti-PDK4 Rabbit Polyclonal Antibody  
Predicted band size: 46 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

  Anti-PDK4 Rabbit Polyclonal Antibody  
Predicted band size: 46 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

  Rabbit Polyclonal Anti-PDK4 Antibody  
WB Suggested Anti-PDK4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

  Rabbit Polyclonal PDK4 Antibody  
Western Blot: PDK4 Antibody [TA336725] - Western Blot on human heart protein lysate. Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

  Rabbit Polyclonal PDK4 Antibody  
Western Blot: PDK4 Antibody [TA336726] - Western Blot on human heart protein lysate. Rabbit Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDK4 (Center) antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PDK4 - human gene knockout kit via CRISPR

PDK4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PDK4 - human gene knockout kit via CRISPR

PDK4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDK4 - human gene knockout kit via CRISPR

PDK4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDK4 - human gene knockout kit via CRISPR

PDK4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdk4 - mouse gene knockout kit via CRISPR

Pdk4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pdk4 - mouse gene knockout kit via CRISPR

Pdk4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdk4 - mouse gene knockout kit via CRISPR

Pdk4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdk4 - mouse gene knockout kit via CRISPR

Pdk4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PDK4

  Recombinant protein of human pyruvate dehydrogenase kinase, isozyme 4 (PDK4)  
PDK4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PDK4 (mGFP-tagged) - Human pyruvate dehydrogenase kinase, isozyme 4 (PDK4), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDK4 (mGFP-tagged) - Human pyruvate dehydrogenase kinase, isozyme 4 (PDK4), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDK4 (Myc-DDK tagged) - Human pyruvate dehydrogenase kinase, isozyme 4 (PDK4), nuclear gene encoding mitochondrial protein , 200 uL, >10^7 TU/mL

Lenti ORF particles, PDK4 (Myc-DDK tagged) - Human pyruvate dehydrogenase kinase, isozyme 4 (PDK4), nuclear gene encoding mitochondrial protein , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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