PDK4 antibody

Principal name

PDK4 antibody

Alternative names for PDK4 antibody

Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase lipoamide kinase isozyme 4 mitochondrial

SwissProt ID

O54937 (Rat), O70571 (Mouse), Q16654 (Human)

Gene ID

5166 (PDK4), 27273 (Pdk4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of PDK4 antibody

PDK4 (pyruvate dehydrogese kise isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptiol initiation complex responsible for PDK4 activation. Insulin promotes ictivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogese complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.

General readings

Kimura,K., (2006) Genome Res. 16 (1), 55-65

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDK4 (C-term) antibody

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PDK4 Antibody (C-term) (Cat. #TA302046), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDK4 (C-term) antibody

Human Adrenal (formalin-fixed, paraffin-embedded) stained with PDK4 antibody AP22786PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms E, ICC/IF, P, WB
0.2 ml / €470.00
  Acris Antibodies GmbH

PDK4 antibody

Predicted band size: 46 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

Predicted band size: 46 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

WB Suggested Anti-PDK4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

Western Blot: PDK4 Antibody [TA336725] - Western Blot on human heart protein lysate. Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

Western Blot: PDK4 Antibody [TA336726] - Western Blot on human heart protein lysate. Rabbit Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDK4 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDK4 (Center) antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PDK4 antibody

PDK4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PDK4 antibody

PDK4 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PDK4 antibody

Western Blot analysis of PDK4 expression in transfected 293T cell line (H00005166-T03) by PDK4 MaxPab polyclonal antibody.

Lane 1: PDK4 transfected lysate(46.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PDK4 antibody

Detection limit for recombinant GST tagged PDK4 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 1A10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PDK4 antibody

Western blot analysis of PDK4 ( arrow ) using PDK4 polyclonal antibody ( Cat # PAB4564 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PDK4 gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PDK4 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 polyclonal antibody ( Cat # PAB4565 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PDK4 antibody

Western blot analysis of PDK4 polyclonal antibody ( Cat # PAB4566 ) in HL-60 cell line lysates ( 35 µg/lane ) . PDK4 ( arrow ) was detected using the purified PDK4 polyclonal antibody ( Cat # PAB4566 ) ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PDK4 antibody

PDK4 Rabbit Hu, Ms, Rt E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PDK4 antibody

Human Muscle; PDK4 antibody - N-terminal region (ARP32787_P050) in Human Muscle cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

PDK4 antibody

PDK4 Rabbit Biotin Bov, Hu, Ms, Rt WB
0.1 ml / €360.00
  Novus Biologicals Inc.

PDK4 antibody

PDK4 Rabbit DyLight488 Bov, Hu, Ms, Rt WB
0.1 ml / €390.00
  Novus Biologicals Inc.

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