PDPK1 antibody

Principal name

PDPK1 antibody

Alternative names for PDPK1 antibody

PDK-1, PDK1, 3-phosphoinositide-dependent proteinkinase 1, hPDK1, PDPK1

SwissProt ID

O15530 (Human), O55173 (Rat), Q9W0V1 (Drome), Q9Y1J3 (Caeel), Q9Z2A0 (Mouse)

Gene ID

5170 (PDPK1), 18607 (Pdpk1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Ze (Zebrafish), Bov (Bovine), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Frozen Sections (C)

Background of PDPK1 antibody

PDK1 (3-Phosphoinositide-dependent protein kinase 1) is a serine/threonine protein kinase known to phosphorylate and activate various signal transduction pathways: Akt/PKB (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC, and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P3 and PtdIns(3,4)P2, which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1, 2). Unlike other phosphorylation sites on PDK1, only phosphorylation on the activation loop at Ser241 is necessary for PDK1 activity; a single mutation to Ala abolishes its activity (3). PDK1 can also autophosphorylate Ser241, leading to its own activation (3).

General readings

Lin,H.J., (2005) Br. J. Cancer 93 (12), 1372-1381

  1. Quick-Filters:
  2. Highly Validated (3)

Items 1 to 20 of 53 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PDPK1 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 antibody (AP02549PU-S; AP02549PU-N). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

PDPK1 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 antibody (AP02549PU-S; AP02549PU-N). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

PDPK1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between RPS6KA3 and PDPK1 HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal 1:1200 and anti-PDPK1 mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse Purified Hu, Ms E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PDPK1 antibody

AP15725PU PDPK1 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

PDPK1 antibody

AP15725PU PDPK1 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
1 ml / €440.00
  Acris Antibodies GmbH

PDPK1 antibody

AP15725PU PDPK1 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
0.1 ml / €180.00
  Acris Antibodies GmbH

PDPK1 antibody

Western blot using affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 µg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4° C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] (p/n 611-1302) and chemiluminescent detection is recommended. Other detection systems will yield similar results. Rabbit Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDPK1 antibody

Predicted band size: 63 kDa . Positive control: MCF, 293 and PC12 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2,3: MCF, 293 and PC12 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDPK1 antibody

Western blot analysis of PDK1 expression in EGF treated MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PDPK1 antibody

Western blot analysis of extracts of various cell lines, using PDPK1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDPK1 antibody

ELISA: PDPK1 Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

PDPK1 antibody

Western Blot analysis of PDK-1 expression with Jurkat cell lysate. Rabbit Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

PDPK1 pSer241 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 antibody (Phospho-Ser241) Cat. AP02307PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

PDPK1 pSer241 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 antibody (Phospho-Ser241) Cat. AP02307PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

PDPK1 pSer241 (236-248) antibody

Figure 1. Staining PDPK1 in Kidney by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Hu E, P
50 µg / €470.00
  Acris Antibodies GmbH

PDPK1 pSer396 antibody

Dot blot analysis of anti-PDPK1-pSer396 Phospho-specific Pab (CAt#AP12874PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

PDPK1 antibody

Human Testis, Spermatogonia: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

PDPK1 pTyr9 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody AP55743PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
0.1 ml / €340.00
  Acris Antibodies GmbH

PDPK1 pTyr9 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody AP55743PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P
50 µl / €270.00
  Acris Antibodies GmbH

PDPK1 antibody

PDPK1 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

Items 1 to 20 of 53 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn