PEA15 antibody

Principal name

PEA15 antibody

Alternative names for PEA15 antibody

Astrocytic phosphoprotein PEA-15, PED, Phosphoprotein enriched in diabetes

SwissProt ID

Q15121 (Human), Q5U318 (Rat), Q62048 (Mouse)

Gene ID

8682 (PEA15)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Flow Cytometry (F), Frozen Sections (C)

Background of PEA15 antibody

PED/PEA-15 (Phosphoprotein Enriched in Diabetes/Phosphoprotein Enriched in Astrocytes - 15 kDa) is a widely expressed 15 kDa protein comprised of an N-terminal region containing a canonical Death Effector Domain (DED) sequence and a nuclear export signal, and a C-terminal region containing two serine phosphorylation sites. PED/PEA-15 has been implicated in the regulation of multiple cellular processes including apoptosis, integrin activation, and insulin-sensitive glucose transport in insulin-responsive cells. Consistent with this latter function, expression of PED/PEA-15 is increased 2-3-fold in skeletal muscle and adipose cells of patients with Type II diabetes. PED/PEA-15 function is mediated through binding to multiple proteins, including ERK1&2, RSK2, Akt, FADD, and Caspase-8, which, in part, modulates their subcellular localization and access to potential substrates. A major function of PED/PEA-15 may be, therefore, to coordinate inputs from multiple pathways to achieve a unified cellular response. Phosphorylation of both serine 104 (a Protein Kinase C site) and serine 116 (a substrate of CaMKII and Akt) is required for PED/PEA-15 function.

General readings

Renault, F., et al. (2003) The multifunctional protein PEA-15 is involved in the control of apoptosis and cell cycle in astrocytes. Biochem. Pharmacol. 66(8):1581-1588.
Chou, F.L., et al. (2003) PEA-15 binding to ERK1/2 MAP Kinases is required for its modulation of integrin activation. J. Biol. Chem. (in press) ePub Manuscript #M309322200.
Trencia, A., et al. (2003) Protein kinase B/Akt binds and phosphorylates PED/PEA-15, stabilizing its antiapoptotic action. Mol. Cell. Biol. 23(13):4511-4521.
Vaidyanathan, H. and J.W. Ramos (2003) RSK2 activity is regulated by its interaction with PEA-15. J. Biol. Chem. 278(34):32367-32372.
Condorelli, G., et al. (2002) Multiple members of the mitogen-activated protein kinase family are necessary for PED/PEA-15 antiapoptotic function. J. Biol. Chem. 277(13):11013-11018.
Xiao, C., et al. (2002) Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-induced death-inducing signaling complex and its modulation by c-FLIP and PED/PEA-15 in glioma cells. J. Biol. Chem. 277(28):25020-25025.
Formstecher, E., et al. (2001) PEA-15 mediates cytoplasmic sequestration of ERK MAP Kinase. Dev. Cell 1(2):239-250.
Ramos, J.W., et al. (2000) Death effector domain protein PEA-15 potentiates Ras activation of Extracellular Signal Receptor-Activated Kinase by an adhesion-independent mechanism. Mol. Biol. Cell 11(9):2863-2872.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PEA15 antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue using PEA-15 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PEA15 antibody

Immunohistochemistry (IHC) analyzes of PEA-15 antibody (Cat.-No.: AP06559PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PEA15 (C-term) antibody

(LEFT) Western blot analysis of PEA-15 Antibody (C-term) (AP18148PU-N) in T47D cell line lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of PEA-15 Antibody (C-term) (AP18148PU-N) in mouse lung tissue lysates (35ug/lane).PEA-15 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PEA15 pSer104 antibody

Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody AP55709PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

PEA15 pSer104 antibody

Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody AP55709PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €270.00
  Acris Antibodies GmbH

PEA15 antibody

Western Blot analysis of PEA15 expression in transfected 293T cell line (H00008682-T01) by PEA15 MaxPab polyclonal antibody.

Lane 1: PEA15 transfected lysate(11.00 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PEA15 pSer116 antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using PEA-15 (Phospho-Ser116) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

PEA15 pSer116 antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using PEA-15 (Phospho-Ser116) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

PEA15 pSer104 antibody

Immunohistochemistry analysis of paraffin-embedded human heart, using PEA-15 (Phospho-Ser104) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

PEA15 pSer104 antibody

Immunohistochemistry analysis of paraffin-embedded human heart, using PEA-15 (Phospho-Ser104) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

PEA15 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PEA-15 (Ab-116) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

PEA15 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PEA-15 (Ab-116) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

PEA15 antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using PEA-15 (Ab-104) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

PEA15 antibody

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using PEA-15 (Ab-104) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

PEA15 antibody

Immunofluorescence analysis of MCF7 cells, using PEA15 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

PEA15 antibody

Immunofluorescence analysis of MCF7 cells, using PEA15 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

PEA15 antibody

Transfected MCF7; WB Suggested Anti-PEA15 Antibody . . Positive Control: Lane 1: 20ug EGFP-transfected MCF7 lysate Lane 2: 20ug EGFP-PEA15 transfected MCF7 lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000. Submitted by: Yann Wallez (Sanford-Burnham Medical Research Institute); PEA15 antibody - middle region (ARP48232_P050) in Transfected MCF7 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PEA15 (C-term) antibody

PEA15 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PEA15 antibody

PEA15 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

PEA15 antibody

PEA15 Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

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