PEBP1 / RKIP antibody

Principal name

PEBP1 / RKIP antibody

Alternative names for PEBP1 / RKIP antibody

PEBP-1, PEBP, Prostatic-binding protein, PBP, Raf kinase inhibitor protein, HCNPpp, Phosphatidylethanolamine-binding protein 1

SwissProt ID

P13696 (Bovin), P30086 (Human), P31044 (Rat), P70296 (Mouse), Q3YIX4 (Canfa), Q8MK67 (Rabit)

Gene ID

5037 (PEBP1), 29542 (Pebp1), 23980

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Rb (Rabbit), African clawed frog, Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PEBP1 / RKIP antibody

Raf kise inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kises. RKIP has been shown to complex with Raf-1, MEK, and ERK. Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively termites sigl transmission from Raf-1 to MAP kises. The inhibitory effect of RKIP on MAP kise sigling is elimited by PKC phosphorylation of RKIP at Ser153. PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent interlization of GPCR. RKIP also interacts with modules of the NF-?B pathway, including NF-?B-inducing kise (NIK), TAK1, IKKa and IKKß. These interactions antagonize cytokine-induced activation of the NF-?B pathway. Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion.

General readings

Yeung K et al. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature 401:173-7 (1999).
PUBMED:10490027

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PEBP1 / RKIP antibody

Western blot analysis of testis cell and brian cell lysate,using PEBP1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PEBP1 / RKIP (C-term) antibody

TA302567 staining (0.01µg/ml) of A549 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PEBP1 / RKIP antibody

TA302568 (0.01µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PEBP1 / RKIP antibody

Western blot analysis of extracts from K562 cells and Jurkat cells, using PEBP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PEBP1 / RKIP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEBP1 (RC206355, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEBP1. Mouse IgG1 OTI2D4 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

PEBP1 / RKIP antibody

Figure 1. Western blot of rat brain lysate showing the specific immunolabeling of the ~22k PEBP protein. Rabbit IgG Serum Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

PEBP1 / RKIP (N-term) antibody

(TOP)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.  (BUTTOM)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PBP antibody (N-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PEBP1 / RKIP (Center) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PEBP1 / RKIP (175-187) antibody

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with PEBP1 antibody AP22528PU-N at 1.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Can, Chk, Eq, Fi, Hst, Hu, Insect, Mky, Ms, Op, Por, Prim, Rb, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PEBP1 / RKIP (70-83) antibody

Human Adrenal (formalin-fixed, paraffin-embedded) stained with PEBP1 antibody AP22542PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Can, Chk, Eq, Fi, Hst, Hu, Insect, Mky, Ms, Nematode, Op, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PEBP1 / RKIP antibody

PEBP1 / RKIP Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PEBP1 / RKIP antibody

PBP monoclonal antibody (M01), clone 2G2-1F1. Western Blot analysis of PEBP1 expression in human kidney. Mouse IgG2b 2G2-1F1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PEBP1 / RKIP antibody

PBP monoclonal antibody (M02), clone 1D3-1B6 Western Blot analysis of PBP expression in HL-60 ( Cat # L014V1 ). Mouse IgG1 1D3-1B6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PEBP1 / RKIP antibody

PBP monoclonal antibody (M05), clone M2 Western Blot analysis of PBP expression in HeLa ( Cat # L013V1 ). Mouse IgG M2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PEBP1 / RKIP antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PEBP1 polyclonal antibody ( Cat # PAB3307 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PEBP1 / RKIP antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PEBP1 polyclonal antibody ( Cat # PAB3308 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PEBP1 / RKIP antibody

PEBP1 polyclonal antibody ( Cat # PAB6149 ) ( 1.5µg/ml ) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu, Rt E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PEBP1 / RKIP antibody

PEBP1 polyclonal antibody ( Cat # PAB7136 ) ( 2.5µg/ml ) staining of paraffin embedded human adrenal gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PEBP1 / RKIP antibody

Western blot analysis of extracts from Jurkat/K562 cells, using PEBP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

PEBP1 / RKIP antibody

Western blot analysis of extracts from Jurkat/K562 cells, using PEBP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

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