PELI1 antibody

Principal name

PELI1 antibody

Alternative names for PELI1 antibody

Protein pellino homolog 1, Pellino-1, Pellino 1, PRISM, Pellino-related intracellular-signaling molecule

SwissProt ID

Q8C669 (Mouse), Q96FA3 (Human)

Gene ID

57162 (PELI1), 67245 (Peli1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of PELI1 antibody

Kit Component:
- KN208197G1, PELI1 gRNA vector 1 in pCas-Guide vector
- KN208197G2, PELI1 gRNA vector 2 in pCas-Guide vector
- KN208197D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Moynagh PN. The Pellino family: IRAK E3 ligases with emerging roles in innate immune signaling. Trends Immunol. 2009; 30:33-42.
Jiang Z, Johnson J, Nie H, et al. Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex. J. Biol. Chem. 2003; 278:10952-6
Butler MP, Hanly JA, and Moynagh PN. Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for Pellino proteins being ubiquitin-protein isopeptide ligases. J. Biol. Chem. 2007; 282:29729-37.
Goh ET, Arthur JS, Cheung PC, et al. Identification of the protein kinases that activate the E3 ubiquitin ligase Pellino 1 in the innate immune system. Biochem. J. 2012; 441:339-46

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19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PELI1 (C-term) antibody

  Rabbit Polyclonal Pellino 1 Antibody  
Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PELI1 antibody

  PELI1 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807506) Mouse IgG1 OTI1D9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PELI1 antibody

  PELI1 mouse monoclonal antibody, clone OTI13F8  
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807629)(1:150) Mouse IgG1 OTI13F8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PELI1 antibody

  Rabbit Polyclonal Pellino 1 Antibody  
Western Blot: Pellino 1 Antibody [TA336442] - Analysis of Pellino 1 (Peli1) in human Ramos lysate (A) and mouse RAW lysate (B) using Pellino 1 antibody at 1:1000. Rabbit Purified Hu, Ms P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

PELI1 antibody

  Rabbit Polyclonal Anti-PELI1 Antibody - N-terminal region  
WB Suggested Anti-PELI1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PELI1 antibody

  PELI1 mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1. Mouse IgG1 OTI2H3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PELI1 antibody

  PELI1 mouse monoclonal antibody, clone OTI14F10 (formerly 14F10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807600)(1:150) Mouse IgG2b OTI14F10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PELI1 antibody

  PELI1 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000) Mouse IgG1 OTI1A8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PELI1 antibody

  PELI1 mouse monoclonal antibody, clone OTI3A9 (formerly 3A9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PELI1 (RC208197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PELI1.(1:2000) Mouse IgG1 OTI3A9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PELI1 antibody

  PELI1 mouse monoclonal antibody, clone OTI10D1 (formerly 10D1)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PELI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807741)(1:150) Mouse IgG1 OTI10D1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

PELI1 - human gene knockout kit via CRISPR

PELI1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PELI1 - human gene knockout kit via CRISPR

PELI1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PELI1 - human gene knockout kit via CRISPR

PELI1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PELI1 - human gene knockout kit via CRISPR

PELI1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PELI1

  Recombinant protein of human pellino homolog 1 (Drosophila) (PELI1)  
PELI1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PELI1 (mGFP-tagged) - Human pellino homolog 1 (Drosophila) (PELI1), 200 uL, >10^7 TU/mL

Lenti ORF particles, PELI1 (mGFP-tagged) - Human pellino homolog 1 (Drosophila) (PELI1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PELI1 (Myc-DDK tagged) - Human pellino homolog 1 (Drosophila) (PELI1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PELI1 (Myc-DDK tagged) - Human pellino homolog 1 (Drosophila) (PELI1) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Peli1 (GFP-tagged) - Mouse pellino 1 (Peli1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Peli1 (GFP-tagged) - Mouse pellino 1 (Peli1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Peli1 (Myc-DDK-tagged) - Mouse pellino 1 (Peli1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Peli1 (Myc-DDK-tagged) - Mouse pellino 1 (Peli1), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

19 Item(s)

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