Pepsin antibody

Principal name

Pepsin antibody

Alternative names for Pepsin antibody

Pepsin A, PGA, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I, Pepsinogen A, PGA3, PGA4, PGA5

SwissProt ID

P00790 (Human), P00791 (Pig), P00792 (Bovin), P00793 (Chick), Q9GMY6 (Canfa)

Gene ID

643834 (PGA3), 396892 (PGA), 5222 (PGA5), 643847 (PGA4)

Available reactivities

Hu (Human), Por (Porcine), Can (Canine), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Dot blot (Dot), Enzyme Immunoassay (E), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Pepsin antibody

PGA5: Pepsinogen 5, group I (pepsinogen A). Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor,pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates.PGA5 is inhibited by ovUS-1, a uterine serpin.

General readings

Plebani, M., et al. Helicobacter 2(4):172-175(1997)
Fujinaga, M., et al. Protein Sci. 4(5):960-972(1995)
Zwiers, A., et al. Clin. Nephrol. 41(3):153-158(1994)
Ichinose, M., et al. Jpn. J. Cancer Res. 82(6):686-692(1991)
Athauda, S.B., et al. J. Biochem. 106(5):920-927(1989)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Pepsin antibody

Immunohistochemistry analysis of Pepsin A antibody Cat.-No AP20789PU-N in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Pepsin antibody

Pepsin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €300.00
  SICGEN

Pepsin antibody

  Anti-PGA5 Rabbit Polyclonal Antibody  
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Pepsin antibody

  Anti-PGA5 Rabbit Polyclonal Antibody  
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Human liver cancer tissue lysate Lysate: 60 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Pepsin antibody

  Anti-PGA4 Rabbit Polyclonal Antibody  
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Pepsin antibody

  Anti-PGA4 Rabbit Polyclonal Antibody  
Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Pepsin antibody

  Rabbit Polyclonal Anti-PGA4 Antibody  
WB Suggested Anti-PGA4 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Liver Rabbit IgG Purified Bov, Hu, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

Pepsin antibody

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 mouse mAb with DAB staining. Mouse IgG1 2C1 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Pepsin antibody

Pepsin Rabbit IgG Azide Free Por Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €240.00
  Acris Antibodies GmbH

Pepsin antibody

Pepsin Rabbit IgG Biotin Por Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €350.00
  Acris Antibodies GmbH

Pepsin (N-term) antibody

Western blot analysis of Pepsin Antibody (N-term) Cat.-No AP53266PU-N  in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the PGA3 antibody detected the PGA3 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Pepsin (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with PGA4 Antibody (C-term) Cat.-No AP53267PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Pepsin (C-term) antibody

Western blot analysis of Pepsin Antibody (C-term) Cat.-No AP53268PU-N in NCI-H292 cell line lysates (35ug/lane). This demonstrates the PGA5 antibody detected the PGA5 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Pepsin antibody

Pepsin Goat Serum Por C, E, IP, P, WB
2 ml / €1,180.00
  Acris Antibodies GmbH

Pepsin antibody

Pepsin Goat IgG Biotin Por C, E, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Pepsin antibody

Pepsin Goat IgG HRP Por C, E, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Pepsin antibody

Immunohistochemistry of goat anti-pepsin antibody 1:100 Goat IgG Purified Por C, E, IP, P, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PGA4 - human gene knockout kit via CRISPR

PGA4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PGA4 - human gene knockout kit via CRISPR

PGA4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PGA4 - human gene knockout kit via CRISPR

PGA4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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