Perilipin-1 antibody

Principal name

Perilipin-1 antibody

Alternative names for Perilipin-1 antibody

Perilipin, PLIN, PLIN1, PLIN-1, PERI, Lipid Droplet-Associated Protein

SwissProt ID

O60240 (Human), P43884 (Rat), Q8CGN5 (Mouse)

Gene ID

5346 (PLIN1), 103968 (Plin1), 25629

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), Sh (Sheep), Bt (Bat), Eq (Equine), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Bear, GP (Guinea Pig)

Available hosts

Guinea Pig, Goat, Mouse, Rabbit

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of Perilipin-1 antibody

Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids.
Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and Perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, Perilipin is phosphorylated by protein kinase A. Once activated, Perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body.
Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.

General readings

Wolins NE, Brasaemle DL, Bickel PE.
A proposed model of fat packaging by exchangeable lipid droplet proteins.
FEBS Lett. 2006 Oct 9;580(23):5484-91. Epub 2006 Sep 1. Review.
PMID: 16962104

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Perilipin-1 antibody

Perilipin-1 Guinea Pig Serum Bov, Hu, Ms, Rt C, P, WB
0.1 ml / €350.00
  Acris Antibodies GmbH

Perilipin-1 antibody

  Goat Polyclonal Antibody against PLIN  
TA303218 (0.01µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Perilipin-1 (C-term) antibody

  Goat Polyclonal Antibody against PLIN (C-terminal)  
TA303219 (0.01µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Perilipin-1 antibody

  Rabbit Polyclonal Perilipin Antibody  
Western Blot: Perilipin Antibody -WB detection of Perilipin in human liver samples. Rabbit Purified Bov, Hu, Ms, Por, Rt, Sh WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Perilipin-1 antibody

  Rabbit polyclonal anti-Perilipin A antibody  
Western blot analysis of Perilipin A in Jurkat cell lysate. Rabbit Purified Hu, Ms, Rt WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Perilipin-1 antibody

  Rabbit Polyclonal Anti-PLIN Antibody  
WB Suggested Anti-PLIN Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Sh, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Perilipin-1 antibody

  Rabbit Polyclonal Anti-PLIN1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fat tissue, Primary antibody: (PLIN1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Perilipin-1 antibody

  Rabbit Polyclonal Anti-PLIN1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fat tissue, Primary antibody: (PLIN1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Perilipin-1 antibody

  Rabbit Polyclonal Anti-PLIN1 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (PLIN1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Perilipin-1 antibody

Perilipin-1 Mouse IgG1 PERI112.17 Supernatant Bov, Hu, Ms, Rt C, ICC/IF, P, WB
5 ml / €300.00
  Acris Antibodies GmbH

Perilipin-1 (488-499) antibody

Human Colon (formalin-fixed, paraffin-embedded) stained with PLIN antibody AP22521PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Por, Prim, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Perilipin-1 (C-term) antibody

Human Colon (formalin-fixed, paraffin-embedded) stained with PLIN antibody AP22522PU-N at 4 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Purified Can, Hu, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Perilipin-1 (C-term) antibody

Perilipin-1 Guinea Pig Serum Bov, Hu C, P, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Plin1 - mouse gene knockout kit via CRISPR

Plin1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Plin1 - mouse gene knockout kit via CRISPR

Plin1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Plin1 - mouse gene knockout kit via CRISPR

Plin1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Perilipin-1 (transcript variant 1)

  Recombinant protein of human perilipin (PLIN), transcript variant 1  
Perilipin-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PLIN1 overexpression lysate

PLIN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

PLIN1 overexpression lysate

PLIN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Plin1 (GFP-tagged) - Mouse perilipin 1 (Plin1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Plin1 (GFP-tagged) - Mouse perilipin 1 (Plin1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,140.00
  OriGene Technologies, Inc.

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