Peripherin antibody

Principal name

Peripherin antibody

Alternative names for Peripherin antibody

PRPH, NEF4, PRPH1

SwissProt ID

P15331 (Mouse), P21807 (Rat), P41219 (Human), P48676 (Xenla), P84117 (Perai)

Gene ID

5630 (PRPH), 19132 (Prph), 24688

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mam (Mammalia), Chk (Chicken), Can (Canine), Fe (Feline), Bov (Bovine), Por (Porcine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Chicken, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of Peripherin antibody

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Peripherin is a class III intermediate filament subunit protein found in certain classes of neuron, most of which are located in the peripheral nervous system. Certain sensory neurons in the brain stem express peripherin normally, as do a few cortical and hippocampal neurons. Interestingly, peripherin protein is upregulated in the lower motor neurons that degenerate in Amyotrophic Lateral Sclerosis (ALS), focusing attention on peripherin as a potential contributory factor to this serious human neurodegenerative disease. Upregulation of peripherin mRNA and protein expression is seen in a variety of damage and disease states. To raise this particular antibody rat peripherin was expressed in bacteria and purified from inclusion bodies by dissolving in 6M urea followed by preparative gel electrophoresis. The pure protein was used to raise antibody in chicken by standard procedures and immunoglobulin was extracted from egg yolk. The resulting polyclonal antibody belongs to the IgY subclass. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G. The IgY total concentration is is 26mg/ml.

  1. Quick-Filters:
  2. Highly Validated (5)

Items 1 to 20 of 52 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Peripherin antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRPH mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800094)(1:200) Mouse IgG1 UMAB202 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Peripherin antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRPH mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800094)(1:200) Mouse IgG1 UMAB202 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Peripherin antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806181) Mouse IgG1 OTI3F4 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Peripherin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806182) Mouse IgG1 OTI2D5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Peripherin antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806183) Mouse IgG1 OTI1E10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Peripherin antibody

Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Peripherin antibody. TA308940 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 57k peripherin protein. Mouse IgG1 7C5 Supernatant Mam C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 57k peripherin protein. Chicken Ig Ig Fraction Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Western blot analysis of extracts from HepG2 cells, using Peripherin antibody (#TA312305).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using PRPH antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and liver cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PRPH antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peripherin (C-term) antibody

TA329072 (0.1µg/ml) staining of Rat Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Western Blot: Peripherin Antibody [TA336607] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000).  The faint 48 kDa band is a peripherin molecule. Rabbit Purified Hu, Mam, Ms, Rt C, E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L NBP1-05218 (red channel). A clas Mouse IgG1 7C5 Purified Chk, Hu, Mam, Ms, Rt ICC/IF, WB
0.5 ml / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Immunocytochemistry/Immunofluorescence: Peripherin Antibody [TA336714] - shows rat mixed neuron/glial cultures stained with TA336714, our chicken polyclonal antibody to peripherin (green channel) and rabbit antibody to the neurofilament subunit alpha-inte Chicken IgY Purified Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

Western blot analysis on HepG2 cell lysate using Peripherin Antibody Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Peripherin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPH (RC207561, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPH. Mouse IgG1 OTI3H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Peripherin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPH (RC207561, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPH. Mouse IgG1 OTI7D8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Peripherin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPH (RC207561, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPH. Mouse IgG1 OTI4A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Peripherin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPH (RC207561, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPH. Mouse IgG2b OTI7E4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Items 1 to 20 of 52 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn