Peroxiredoxin-3 / PRDX3 antibody

Principal name

Peroxiredoxin-3 / PRDX3 antibody

Alternative names for Peroxiredoxin-3 / PRDX3 antibody

PRDX-3, Prx3, Prx-3, PRX-III, AOP-1, AOP1, HBC189, Thioredoxin-dependent peroxide reductase mitochondrial, Antioxidant protein 1, Protein MER5 homolog

SwissProt ID

P20108 (Mouse), P30048 (Human), P35705 (Bovin), Q9Z0V6 (Rat)

Gene ID

10935 (PRDX3), 11757 (Prdx3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair)

Background of Peroxiredoxin-3 / PRDX3 antibody

PRDX3 is a protein with antioxidant function and is localized in the mitochondrion. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.

General readings

Cox AG., et al. (2009). Biochemistry. 48(27):6495-501.
Rhee SG., et al. (2005). Free Radic Biol Med. 38(12):1543-52.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Peroxiredoxin-3 / PRDX3 antibody

Predicted band size: 28kDa. Positive control: Hela, 293T, MCF7 and Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1-4: Hela, 293T, MCF7 and Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peroxiredoxin-3 / PRDX3 antibody

Predicted band size: 28kDa. Positive control: Hela, 293T and MCF7 cell, human fetal brain tissue and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1-5: Hela, 293T and MCF7 cell, human fetal brain tissue and Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 2 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peroxiredoxin-3 / PRDX3 antibody

Predicted band size: 28 kDa . Positive control: Hela and 293T cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane1: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/800 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Peroxiredoxin-3 / PRDX3 antibody

WB Suggested Anti-PRDX3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysatePRDX3 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Peroxiredoxin-3 / PRDX3 (N-term) antibody

Western blot analysis of PRDX3 Antibody (N-term) (AP17663PU-N) in NCI-H460,MDA-MB231,HepG2 cell line lysates (35ug/lane). PRDX3 (arrow) was detected using the purified Pab.(2ug/ml) Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Peroxiredoxin-3 / PRDX3 antibody

Immunohistochemistry (IHC) analyzes of PRX III antibody (Cat.-No.: AP20593PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Peroxiredoxin-3 / PRDX3 antibody

Human Prostate (formalin-fixed, paraffin-embedded) stained with PRDX3 antibody AP22617PU-N at 1/250 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu, Ms, Rt IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Peroxiredoxin-3 / PRDX3 antibody

Peroxiredoxin-3 / PRDX3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

Immunofluorescence of purified MaxPab antibody to PRDX3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

Peroxiredoxin-3 / PRDX3 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

PRDX3 MaxPab rabbit polyclonal antibody. Western Blot analysis of PRDX3 expression in mouse spleen. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

Immunofluorescence of monoclonal antibody to PRDX3 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG1 1E3-B2 Purified Hu E, ICC/IF
0.1 mg / €370.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

Immunoprecipitation analysis of 293T cell lysates. Lane 1 : Input and lane 2 : Precipitates. Immunoblotting with PRDX3 monoclonal antibody, clone 2B11 ( Cat # MAB0696 ). Mouse IgG2a 2B11 Hu E, IP
0.1 ml / €390.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

Immunoblot analysis of cell lysates. Lane 1 : Hela cell lysates, lane 2 : 293T cell lysate and lane 3 : Jurkat cell lysate. Mouse IgG1 4G10 Hu E, IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

Immunoblot analysis of cell lysates.
Lane 1 : HeLa cell lysate.
 Lnae 2 : 293T cell lysate.
Lane 3 : Mouse liver tissue lysate.
Lane 4 : Rat liver tissue lysate.
Lane 5 : PC-12 cell lysate.
Lane 6 : L929 cell lysate. Mouse AF17D2FF Purified Hu, Ms, Rt IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

Immunoblot analysis of cell and tissue lysates. Lane 1 : HeLa cell lysate. Lane 2 : Mouse liver. Lane 3 : Rat brain. Rabbit Hu IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Peroxiredoxin-3 / PRDX3 antibody

Human Bronchial Epithelial Tissue; Rabbit Anti-PRDX3 Antibody . Catalog Number: ARP52341_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue . Observed Staining: Cytoplasmic . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-PRDX3 Antibody (ARP52341_P050) ) in Human Bronchial Epithelial Tissue using Immunohistochemistry Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Peroxiredoxin-3 / PRDX3 antibody

Peroxiredoxin-3 / PRDX3 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

Peroxiredoxin-3 / PRDX3 antibody

Peroxiredoxin-3 / PRDX3 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Peroxiredoxin-3 / PRDX3 antibody

Immunohistochemical analysis of Peroxiredoxin 3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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