PFKFB4 antibody
Principal name
PFKFB4 antibody
Alternative names for PFKFB4 antibody
6-phosphofructo-2-kinase, 6PF-2-K, PFK/FBPase 4, fructose-2,6-biphosphatase 4, Fru-2,6-P2ase testis-type isozyme
Ncbi ID
9606, NP_004558, NP_004558.1, NM_004567, NP_766607.3, BC057594, NM_173019, NM_019333
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of PFKFB4 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PFKFB4 polyclonal antibody ( Cat # PAB4031 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
The PFKFB4 polyclonal antibody ( Cat # PAB4031 ) is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.
Left: Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4 Antibody Cat.-No AP15154PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Right: Formalin-fixed, paraffin-embedded human cancer tissue reacted with PFKFB4 Antibody Cat.-No AP15154PU-N, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot analsis using PFKFB4 Antibody Cat.-No AP15154PU-N to detect PFKFB4 in mouse brain tissue lysate.
FTS down-regulates HIF-1 and HIF-1-regulated proteins in GBM cells. GBM cell lines were incubated under hypoxia (1% O2) for the indicated periods with FTS and then processed for the determination of PFKFB4 by Western Blot. Samples of total cell lysates were also immunoblotted with anti-Beta-Tubulin antibody.
HEK293, H1299; WB Suggested Anti-PFKFB4 Antibody . . Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000. Submitted by: Jose Luis Rosa, Universitat de Barcelona; PFKFB4 antibody - N-terminal region (ARP56584_P050) in HEK293, H1299 cells using Western Blot
human primary tumor; WB Suggested Anti-PFKFB4 Antibody . . Positive Control: Lane1: 10ug human primary tumor lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti-rabbit-HRP . Secondry Antibody Dilution : 1:5000. Submitted by: Anonymous; PFKFB4 antibody - N-terminal region (ARP56584_P050) in human primary tumor cells using Western Blot
Anti-PFKFB4 mouse monoclonal antibody (TA500809) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKFB4(RC201573).
Immunofluorescent staining of HepG2 cells using anti-PFKFB4 mouse monoclonal antibody (TA500809).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKFB4 (RC201573, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PFKFB4.
WB Suggested Anti-PFKFB4 Antibody ; Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate; Primary Antibody Dilution : 1:1000; Secondary Antibody : Goat anti-rabbit-HRP; Secondry Antibody Dilution : 1:5000;
WB Suggested Anti-PFKFB4 Antibody ; Positive Control: Lane1: 10ug human primary tumor lysate; Primary Antibody Dilution : 1:1000; Secondary Antibody : Anti-rabbit-HRP; Secondry Antibody Dilution : 1:5000;
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA342218 | PFKFB4 antibody |
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| Rabbit polyclonal Anti-PFKFB4 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA500809 | PFKFB4 antibody |
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| anti-PFKFB4 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8) | |||||||
 |
Mouse | IgG2a | OTI1C8 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP15154PU-N | PFKFB4 antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN201573 | PFKFB4 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201573BN | PFKFB4 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201573LP | PFKFB4 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN201573RB | PFKFB4 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN313142 | Pfkfb4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN313142BN | Pfkfb4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN313142LP | Pfkfb4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN313142RB | Pfkfb4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301573 | PFKFB4 |
||||
| Recombinant protein of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401448 | PFKFB4 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC201573L2V | Lenti ORF particles, PFKFB4 (mGFP-tagged) - Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC201573L1V | Lenti ORF particles, PFKFB4 (Myc-DDK tagged) - Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR207244L2V | Lenti ORF particles, Pfkfb4 (GFP-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,206.00
|
|
| OriGene Technologies, Inc. | ||
| MR207244L1V | Lenti ORF particles, Pfkfb4 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (cDNA clone MGC:67170 IMAGE:6810667), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| MR215696L1V | Lenti ORF particles, Pfkfb4 (Myc-DDK-tagged) - Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€989.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214187L2V | Lenti ORF particles, Pfkfb4 (GFP-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,113.00
|
|
| OriGene Technologies, Inc. | ||
| RR214187L1V | Lenti ORF particles, Pfkfb4 (Myc-DDK-tagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€993.00
|
|
| OriGene Technologies, Inc. | ||
