PFKM antibody

Principal name

PFKM antibody

Alternative names for PFKM antibody

Phosphofructokinase-M, Phosphohexokinase, Phosphofructokinase 1, PFK1, PFK-1, PFK-A, PFKX, GSD7, 6-phosphofructokinase muscle type, Phosphofructo-1-kinase isozyme A

SwissProt ID

P00511 (Rabit), P08237 (Human), P47858 (Rat), P52034 (Drome), P52784 (Canfa), Q0IIG5 (Bovin), Q2HYU2 (Pig), Q867C9 (Horse)

Gene ID

5213 (PFKM)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Rb (Rabbit), Can (Canine), Por (Porcine), Xen (Xenopus), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Sheep, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (R)

Background of PFKM antibody

The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Martínez-Costa OH., et al. (2007) FEBS Lett. 581(16):3033-8.
Kim JW., et al. (2004) Mol Cell Biol. 24(13):5923-36.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Fructose 6 Phosphate Kinase antibody

Fructose 6 Phosphate Kinase Goat Biotin Rb E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Fructose 6 Phosphate Kinase antibody

Fructose 6 Phosphate Kinase Goat HRP Rb E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Fructose 6 Phosphate Kinase antibody

Fructose 6 Phosphate Kinase Goat Purified Rb E, ICC/IF, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

PFKM antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308655 diluted at 1:2000. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PFKM antibody

Western blot analysis of extracts of various cell lines, using PFKM antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PFKM antibody

WB Suggested Anti-PFKM Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PFKM antibody

The HepG2 cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human PFKM antibody Cat.-No AM50026PU (1/500~1/2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT2F11 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PFKM antibody

The HepG2 cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Human PFKM antibody Cat.-No AM50026PU (1/500~1/2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2a AT2F11 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

PFKM (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PFKM (C-term) antibody

(TOP)Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (BUTTOM)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PFKM antibody

PFKM Sheep IgG Azide Free Rb Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

PFKM antibody

PFKM Sheep IgG Biotin Rb Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

PFKM antibody

PFKM Sheep IgG Aff - Purified Rb Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

PFKM antibody

PFKM MaxPab polyclonal antibody. Western Blot analysis of PFKM expression in rat brain. Mouse Purified Hu, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PFKM antibody

PFKM Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PFKM antibody

PFKM MaxPab rabbit polyclonal antibody. Western Blot analysis of PFKM expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PFKM antibody

PFKM Mouse IgG 2B11 Purified Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

PFKM antibody

PFKM monoclonal antibody (M04), clone 1D1. Western Blot analysis of PFKM expression in K-562 ( Cat # L009V1 ). Mouse IgG2a 1D1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PFKM antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with PFKM polyclonal antibody ( Cat # PAB2141 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PFKM antibody

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM polyclonal antibody ( Cat # PAB2142 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

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