PGAM1 antibody

Principal name

PGAM1 antibody

Alternative names for PGAM1 antibody

PGAM 1, Phosphoglycerate mutase 1, PGAMA, PGAM-B, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B

SwissProt ID

P18669 (Human), P25113 (Rat), Q3SZ62 (Bovin), Q5ZLN1 (Chick), Q9DBJ1 (Mouse)

Gene ID

5223 (PGAM1), 18648 (Pgam1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Chimp (Chimpanzee), Chk (Chicken), Mky (Monkey), Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of PGAM1 antibody

PGAM1 (phosphoglycerate mutase 1) is an enzyme that regulates a unique step in glycolysis, and most of the glycolytic intermediates that are used as precursors for abolic biosynthesis are upstream of this step. PGAM1 catalyzes the interconversion of 3- phosphoglycerate (3-PGA) to 2- phosphoglycerate (2-PGA), and becomes activated or "primed" by an intrinsic phosphatase activity that converts 2,3-bisphosphoglycerate (2, 3-BPGA) to 2-PGA, phosphorylating the active site histidine in the process. The phosphohistidine is essential to carryout the mutase reaction, converting 3-PGA to 2-PGA in glycolysis. Interestingly, PGAM1 is the rate-limiting enzyme of glycolysis in tumor cells, heart tissue, and leukocytes, and its inhibition by epoxide inhibitors is lethal to cancer cells. In many cancers, including hepatocellular carcinoma and colorectal cancer, PGAM1 activity is increased compared to that in the normal tissues, wherein its expression is upregulated through loss of TP53 as TP53 negatively regulates PGAM1 gene expression.

General readings

General / background references:Sakoda S et al. Isolation of a cDNA encoding the B isozyme of human phosphoglycerate mutase (PGAM) and characterization of the PGAM gene family. J Biol Chem 263:16899-905 (1988). PubMed PMID: 2846553

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PGAM1 antibody

Sample(30 ug of whole cell lysate). A:293T. 12% SDS PAGE. TA308372 diluted at 1:500 Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PGAM1 antibody

Sample (50 ug of whole cell lysate). A: mouse brain. 12% SDS PAGE. TA308373 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PGAM1 antibody

Western Blot: PGAM1 Antibody [TA336785] - Analysis of PGAM1 in Jurkat whole cell extracts Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PGAM1 antibody

Host: Rabbit; Target Name: PGAM1; Sample Tissue: Fetal Brain lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PGAM1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : 293T cell lysate 
Lane 2. : Jurkat cell lysate 
Lane 3. : Raji cell lysate 
Lane 4. : A431 cell lysate 
Lane 5. : HeLa cell lysate Mouse IgG2a AT1G4 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

PGAM1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CBR1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : 293T cell lysate 
Lane 2. : Jurkat cell lysate 
Lane 3. : Raji cell lysate 
Lane 4. : A431 cell lysate 
Lane 5. : HeLa cell lysate Mouse IgG2a AT1G4 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

PGAM1 antibody

PGAM1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PGAM1 antibody

PGAM1 monoclonal antibody (M01), clone 2G1-A6 Western Blot analysis of PGAM1 expression in Jurkat ( Cat # L017V1 ). Mouse IgG1 2G1-A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PGAM1 antibody

Detection limit for recombinant GST tagged PGAM1 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 4F5-D8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

PGAM1 antibody

Western Blot: PGAM1/2/4  Antibody [NB100-774] - NB100-774 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PGAM1 antibody

PGAM1 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

PGAM1 antibody

PGAM1 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

PGAM1 antibody

PGAM1 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

PGAM1 antibody

PGAM1 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

PGAM1 antibody

PGAM1 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

PGAM1 antibody

PGAM1 Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
25 µl / €160.00
  Novus Biologicals Inc.

PGAM1/ PGAM2 / PGAM4 (C-term) antibody

TA302705 staining (0.5µg/ml) of human liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
20 µg / €200.00
  Acris Antibodies GmbH

PGAM1/ PGAM2 / PGAM4 (C-term) antibody

TA302705 staining (0.5µg/ml) of human liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PGAM1/ PGAM2 / PGAM4 antibody

The PGAM1/PGAM2/PGAM4 polyclonal antibody ( Cat # PAB6005 ) staining ( 0.5 µg/ml ) of human liver lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Purified Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PGAM1

PGAM1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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