PHF12 / Pf1 antibody

Principal name

PHF12 / Pf1 antibody

Alternative names for PHF12 / Pf1 antibody

PHD finger protein 12, PHD factor 1, KIAA1523

SwissProt ID

Q5SPL2 (Mouse), Q96QT6 (Human)

Gene ID

57649 (PHF12)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E)

Background of PHF12 / Pf1 antibody

PHD factor 1 (PF1) was identified as a protein that interacts with mSin3A-histone deacetylase corepressor, a multiprotein complex that mediates transcriptional repression. PF1 is a plant homeodomain zinc finger protein that also bears a forkhead-associated (FHA) domain. PF1 appears to facilitate the recruitment of the mSin3A complex to DNA for transcriptional repression. It has also been shown to interact with the histone deacetylase containing corepressor transducin-like enhancer of split (TLE) and may play a role in bridging the mSin3A and TLE transcriptional networks. Alternate names for PF1 include PHF12, PHD finger protein 12, and KIAA1523.

General readings

Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Yochum, G.S., et al. Mol. Cell. Biol. 22(22):7868-7876(2002)
Yochum, G.S., et al. Mol. Cell. Biol. 21(13):4110-4118(2001)

7 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PHF12 / Pf1 antibody

WB Suggested Anti-PHF12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human brain Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PHF12 / Pf1 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with PHF12 Antibody (C-term) Cat.-No AP53280PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PHF12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PHF12 / Pf1 antibody

Human Brain; WB Suggested Anti-PHF12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; PHF12 antibody - C-terminal region (ARP50416_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PHF12 / Pf1 antibody

Western Blot: PF1 Antibody [NB100-81670] - Detection of Human PF1 by Western Blot of  Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP,  20% of IP loaded) from HeLa cells. Antibodies: Affinity purified  rabbit anti-PF1 antibody NB100-81670 used for IP at 3 mcg/mg  lysate. PF1 was also immunoprecipitated by rabbit anti-PF1  antibody NB100-81671, which recognizes a downstream epitope.  Detection: Chemiluminescence with  an exposure time of 30 seconds. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PHF12 / Pf1 antibody

Western Blot and Immunoprecipitation: PF1 Antibody [NB100-81671] - Detection of Human and Mouse PF1 on HeLa whole cell lysate using NB100-81671. Pf1 was also  immunoprecipitated by rabbit anti-PF1 antibody NB100-81670. Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

PHF12 / Pf1 antibody

Immunohistochemistry-Paraffin: PF1 Antibody [NBP1-89599] - Immunohistochemical staining of human prostate shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

PHF12 overexpression lysate

PHF12 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

7 Item(s)

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