PHF8 / ZNF422 antibody
Principal name
PHF8 / ZNF422 antibody
Alternative names for PHF8 / ZNF422 antibody
PHD finger protein 8, KIAA1111, Zinc finger protein 422, Histone lysine demethylase PHF8
Ncbi ID
9606, NM_001184896, NP_001171825.1, NP_001009544.1, NM_177201, NM_001113354, NM_015107, NM_001184897
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB)
Background of PHF8 / ZNF422 antibody
- Kit Component:
- - KN300336G1, 4921501E09Rik gRNA vector 1 in pCas-Guide vector
- KN300336G2, 4921501E09Rik gRNA vector 2 in pCas-Guide vector
- KN300336D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
E14TG2A ( mouse embryonic stem cells ) were transfected with a conditional allele of PHF8.Nuclear extracts ( 135 µg ) from wild type cells ( WT ) and from 2 clones ( F1, B8 ) with an active and a targeted allele ( F1ko, B8ko ), respectively, were analysed by Western blot using PHF8 polyclonal antibody ( Cat # PAB14120 ).The antibody was diluted 1 : 1,000.
ELISA was performed using a serial dilution of PHF8 polyclonal antibody ( Cat # PAB14120 ), crude serum and Flow Through in antigen coated wells.By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 6,500.
WB using the antibody against PHF8. The antibody was diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human PHF8, crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,500.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA347263 | PHF8 / ZNF422 antibody |
||||||
| Rabbit Polyclonal PHF8 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E |
50 µg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN300336 | 4921501E09Rik - mouse gene knockout kit via CRISPR |
||
 |
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN300336BN | 4921501E09Rik - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN300336LP | 4921501E09Rik - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN300336RB | 4921501E09Rik - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214763 | PHF8 - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214763BN | PHF8 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214763LP | PHF8 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN214763RB | PHF8 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN313215 | Phf8 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN313215BN | Phf8 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN313215LP | Phf8 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN313215RB | Phf8 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY433632 | PHF8 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
