PHGDH antibody

Principal name

PHGDH antibody

Alternative names for PHGDH antibody

PGDH3, 3-PGDH, D-3-phosphoglycerate dehydrogenase

SwissProt ID

A5GFY8 (Pig), O04130 (Arath), O08651 (Rat), O43175 (Human), P0A9T0 (Ecoli), P40054 (Yeast), P87228 (Schpo), Q5EAD2 (Bovin), Q61753 (Mouse)

Gene ID

26227 (PHGDH), 2622759 (dnaA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of PHGDH antibody

The survival and development of central neurons require the supply of trophic factors by glial cells. The trophic actions of glial cells on Purkinje neurons are mediated by L-serine and glycine, which are glia-derived trophic factors synthesized by 3PGDH (1). 3PGDH protein is 544 amino acids in length. Two distinct mRNA transcripts that encode for 3PGDH protein in normal human tissues are dominant 2.1 kb mRNA, which is highly expressed in prostate, testis, ovary, brain, liver, kidney, and pancreas, and weakly expressed in thymus, colon, and heart, and 710 bp mRNA, which is highly expressed in heart and skeletal muscle (2). 3PGDH is regulated at the transcriptional level depending on tissue specificity and cellular proliferative status (2). 3PGDH protein is also highly expressed in adult and fetal brain tissues (3). 3PGDH protein plays an important role in the metabolism, development, and function of the central nervous system (3) and its deficiency is a treatable congential error (4-5) that impairs L-serine biosynthesis which is characterized by congenital microcephaly, psychomotor retardation, and seizures (3).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PHGDH antibody

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 4A3-1D6 Purified Hu E, ICC/IF, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

PHGDH antibody

Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration sup. ug/ml] Mouse IgG1 4A3-1D6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PHGDH antibody

WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysatePHGDH is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PHGDH antibody

WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 293T cell lysatePHGDH is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PHGDH antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806763) Mouse IgG2a OTI5C4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PHGDH antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHGDH (RC203949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHGDH. Mouse IgG2b OTI4A1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PHGDH antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PHGDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806876) Mouse IgG1 OTI9C2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PHGDH (N-term) antibody

(LEFT) Western blot analysis of PHGDH Antibody (N-term) (AP17635PU-N) in mouse brain tissue lysates (35ug/lane). PHGDH (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of PHGDH (arrow) using rabbit polyclonal PHGDH Antibody (N-term) (AP17635PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2) . Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PHGDH antibody

Western Blot analysis of PHGDH expression in transfected 293T cell line (H00026227-T01) by PHGDH MaxPab polyclonal antibody.

Lane 1: PHGDH transfected lysate(58.63 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu, Ms E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PHGDH antibody

PHGDH Mouse Purified Hu, Ms E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PHGDH antibody

PHGDH Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PHGDH antibody

PHGDH MaxPab rabbit polyclonal antibody. Western Blot analysis of PHGDH expression in human kidney. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

PHGDH antibody

PHGDH Mouse IgG1 4D5-1F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PHGDH antibody

Human 293T; WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. PHGDH is supported by BioGPS gene expression data to be expressed in HEK293T.; PHGDH antibody - middle region (ARP54801_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PHGDH antibody

Human 293T; WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 293T cell lysate. PHGDH is supported by BioGPS gene expression data to be expressed in HEK293T.; PHGDH antibody - middle region (ARP54802_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PHGDH antibody

Western Blot: PHGDH Antibody [NBP1-32201]-(30 µg of whole cell lysate) A:293T B:A431 C:HeLa S3 7.5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

PHGDH antibody

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87310] - Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblasts. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

PHGDH antibody

Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PHGDH

PHGDH Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PHGDH (full length, N-term HIS tag)

PHGDH Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

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