PHLDA3 antibody

Principal name

PHLDA3 antibody

Alternative names for PHLDA3 antibody

TIH1, Pleckstrin homology-like domain family A member 3, TDAG51/Ipl homologue 1

SwissProt ID

Q5PQT7 (Rat), Q8AW35 (Danre), Q9WV95 (Mouse), Q9Y5J5 (Human)

Gene ID

23612 (PHLDA3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PHLDA3 antibody

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.
The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.

General readings

1. Frank D, Mendelsohn CL, Ciccone E, Svensson K, Ohlsson R, Tycko B. A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting.
Mamm Genome. 1999 Dec;10(12):1150-9.
PMID: 10594239

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PHLDA3 (C-term) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with TA311404 (1ug/ml) in Lane A and probed with anti-MYC Tag (1:1000) in Lane C. Mock-transfected HEK293 probed with TA311404 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PHLDA3 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PHLDA3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

PHLDA3 antibody

WB Suggested Anti-PHLDA3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: 293T cell lysate Rabbit IgG Purified GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PHLDA3 antibody

PHLDA3 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

PHLDA3 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PHLDA3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

PHLDA3 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PHLDA3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

PHLDA3 antibody

Human 293T; WB Suggested Anti-PHLDA3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: 293T cell lysate; PHLDA3 antibody - N-terminal region (ARP54891_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

PHLDA3 antibody

PHLDA3 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €360.00
  Novus Biologicals Inc.

PHLDA3 antibody

PHLDA3 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P
50 µg / €290.00
  Novus Biologicals Inc.

PHLDA3 antibody

Western Blot: PHLDA3 Antibody [NBP1-56772] - This Anti-PHLDA3 antibody was used in Western Blot of 293T tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

PHLDA3 antibody

Immunohistochemistry-Paraffin: PHLDA3 Antibody [NBP1-80683] - Immunohistochemical staining of human small intestine shows generally weak positivity in glandular cells but goblet cells were stronger stained. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PHLDA3

PHLDA3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

PHLDA3

PHLDA3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PHLDA3

PHLDA3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

PHLDA3

PHLDA3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

PHLDA3

PHLDA3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

PHLDA3 overexpression lysate

PHLDA3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

17 Item(s)

per page
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