PHLPP2 antibody

Principal name

PHLPP2 antibody

Alternative names for PHLPP2 antibody

PHLPPL, PHLPP-like, KIAA0931, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like

SwissProt ID

Q6ZVD8 (Human), Q8BXA7 (Mouse)

Gene ID

23035 (PHLPP2), 244650 (Phlpp2)

Available reactivities

Hu (Human)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of PHLPP2 antibody

Kit Component:
- KN313231G1, Phlpp2 gRNA vector 1 in pCas-Guide vector
- KN313231G2, Phlpp2 gRNA vector 2 in pCas-Guide vector
- KN313231D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Brognard J and Newton AC. PHLiPPing the switch on Akt and protein kinase C signaling. Trends Endocrinol. Metab. 2008; 19:223-30.
Gao T, Furnari F and Newton AC. PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol. Cell 2005; 18:13-24.
Brognard J, Sierecki E, Gao T, et al. PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol. Cell 2007; 25:917-31
Gao MH, Miyanohara A, Feramisco JR, et al. Activation of PH-domain leucine-rich protein phosphatase 2 (PHLPP2) by agonist stimulation in cardiac myocytes expressing adenylyl cyclase type 6. Biochem. Biophys. Res. Commun. 2009; 384:193-8.

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PHLPP2 antibody

  Goat Polyclonal Antibody against PHLPPL  
TA303258 (0.1µg/ml) staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PHLPP2 (C-term) antibody

  Rabbit Polyclonal PHLPP2 Antibody  
Western blot analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody at 1 ug/ml in (A) the presence and (B) the absence of blocking peptide. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PHLPP2 antibody

  Rabbit Monoclonal anti-PHLPP2 Antibody  
Western Blot analysis of HEK293T cell lysate with anti-PHLPP2 antibody. Rabbit SP220 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PHLPP2 antibody

  Rabbit Polyclonal PHLPP2 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHLPP2 (RC212846, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PHLPP2. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PHLPP2 (N-term) antibody

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHLPP2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PHLPP2 - human gene knockout kit via CRISPR

PHLPP2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PHLPP2 - human gene knockout kit via CRISPR

PHLPP2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PHLPP2 - human gene knockout kit via CRISPR

PHLPP2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PHLPP2 - human gene knockout kit via CRISPR

PHLPP2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Phlpp2 - mouse gene knockout kit via CRISPR

Phlpp2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Phlpp2 - mouse gene knockout kit via CRISPR

Phlpp2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Phlpp2 - mouse gene knockout kit via CRISPR

Phlpp2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Phlpp2 - mouse gene knockout kit via CRISPR

Phlpp2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PHLPP2

  Recombinant protein of human PH domain and leucine rich repeat protein phosphatase-like (PHLPPL)  
PHLPP2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PHLPP2 overexpression lysate

PHLPP2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PHLPP2 (mGFP-tagged) - Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2), 200 uL, >10^7 TU/mL

Lenti ORF particles, PHLPP2 (mGFP-tagged) - Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2), 200 uL, >10^7 TU/mL  
200 µl / €2,530.00
  OriGene Technologies, Inc.

Lenti ORF particles, PHLPP2 (Myc-DDK tagged) - Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PHLPP2 (Myc-DDK tagged) - Human PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2) , 200 uL, >10^7 TU/mL  
200 µl / €2,280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Phlpp2 (GFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Phlpp2 (GFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), 200 uL, >10^7 TU/mL  
200 µl / €2,250.00
  OriGene Technologies, Inc.

Lenti ORF particles, Phlpp2 (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Phlpp2 (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), 200 uL, >10^7 TU/mL  
200 µl / €2,030.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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