Phospholamban antibody

Principal name

Phospholamban antibody

Alternative names for Phospholamban antibody

Cardiac phospholamban, PLB, PLN

SwissProt ID

A4IFH6 (Bovin), P26677 (Chick), P26678 (Human), P61012 (Canfa), P61013 (Pig), P61014 (Mouse), P61015 (Rabit), P61016 (Rat)

Gene ID

414755 (PLN), 5350, 18821 (Pln)

Available reactivities

Can (Canine), Chk (Chicken), GP (Guinea Pig), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Sh (Sheep), Bov (Bovine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Neutralisation (NEUT), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of Phospholamban antibody

Phospholamban (PLB/PLN) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains cAMP-dependent protein kinase phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. PLB is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

General readings

Reddy LG et al. Purified, reconstituted cardiac Ca2+-ATPase is regulated by phospholamban but not by direct phosphorylation with Ca2+/calmodulin-dependent protein kinase. J Biol Chem 271:14964-70 (1996). PubMed PMID: 8663079
Cougnon M et al. The rat distal colon P-ATPase alpha subunit encodes a ouabain-sensitive H+, K+-ATPase. J Biol Chem 271:7277-80 (1996). PubMed PMID: 8631741
Hu P et al. Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching. J Biol Chem 270:11619-22 (1995). PubMed PMID: 7744801
Rockman HA et al. Molecular and physiological alterations in murine ventricular dysfunction. Proc Natl Acad Sci U S A 91:2694-8 (1994). PubMed PMID: 8146176
Khan I et al. Expression of the internal calcium pump in pregnant rat uterus. Cell Calcium 14:111-7 (1993). PubMed PMID: 8384529
Jorgensen AO et al. The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle. J Cell Biol 120:969-80 (1993). PubMed PMID: 8381786
Jones LR & Field LJ Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum. J Biol Chem 268:11486-8 (1993). PubMed PMID: 8505284
Cantilina T et al. Comparative studies of cardiac and skeletal sarcoplasmic reticulum ATPases. Effect of a phospholamban antibody on enzyme activation by Ca2+. J Biol Chem 268:17018-25 (1993). PubMed PMID: 8349590
Briggs FN et al. Phospholamban expressed in slow-twitch and chronically stimulated fast-twitch muscles minimally affects calcium affinity of sarcoplasmic reticulum Ca(2+)-ATPase. J Biol Chem 267:26056-61 (1992). PubMed PMID: 1464616
Sham JS et al. Phospholamban mediates the beta-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes. Am J Physiol 261:H1344-9 (1991). PubMed PMID: 1681743

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Phospholamban (N-term) antibody

(0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Phospholamban antibody

Western blot analysis of lysate from U266 cell line,using PLN Antibody (N-term)(Cat. #TA328116).TA328116 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Rabbit RB44799 Purified Bov, Hu, Ms, Por, Rb, Rt WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Phospholamban (N-term) antibody

Western blot with Phospholamban Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Phospholamban (pThr7) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using PLB-Thr17 Cat.-No AP53332PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-T17 Antibody for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Phospholamban antibody

Phospholamban Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Phospholamban antibody

Western blot of rat heart tissue lysate with PLN polyclonal antibody ( Cat # PAB13686 ). Rabbit Hu, Ms, Rt WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Phospholamban pSer16/pThr17 antibody

Immunofluorescence analysis of HuvEc cells, using PLB (Phospho-Ser16+Thr17) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

Phospholamban pSer16/pThr17 antibody

Immunofluorescence analysis of HuvEc cells, using PLB (Phospho-Ser16+Thr17) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

Phospholamban antibody

Immunofluorescence analysis of HeLa cells, using PLB (Ab-16/17) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

Phospholamban antibody

Immunofluorescence analysis of HeLa cells, using PLB (Ab-16/17) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

Phospholamban antibody

Phospholamban Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

Phospholamban antibody

Phospholamban Mouse IgG2a 2D12 Purified Can, Chk, GP, Hu, Ms, Por, Rb, Rt, Sh ICC/IF, IP, NEUT, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Phospholamban antibody

Phospholamban Rabbit IgG Aff - Purified Hu E, WB
50 µg / €440.00
  Novus Biologicals Inc.

Phospholamban pSer16 antibody

Western Blot: Phospholamban [S16] Antibody (EPR1911) [NBP1-40742] - Western blot analysis on fetal heart lysates using anti-Phospholamban Phospho (pS16). Cells were either (A) untreated (B) treated with LP. Rabbit IgG EPR1911 Hu, Ms IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Phospholamban antibody

Phospholamban Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Phospholamban antibody

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-85543] - Immunohistochemical staining of human heart muscle shows strong cytoplasmic and nuclear membranous positivity in myocytes. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Phospholamban antibody

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP2-19807] - Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using antibody at 1:250 dilution. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Phospholamban

Phospholamban Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Phospholamban

Phospholamban Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

19 Item(s)

per page
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