Phosphoserine antibody

Principal name

Phosphoserine antibody

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), All Species, var.

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Radioimmunoassay (R), Flow Cytometry (F)

Background of Phosphoserine antibody

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the nucleus. Phosphorylated epitopes may serve as docking sites for the assembley of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosin kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via the interaction of phosphotyrosine residues with SH2 or PTB domains. Antibodies direct against phosphorylated epitopes recognize the phosphorylated amino acid in the context of the surrounding amino acid sequence. Recognition is therefore dependent on 2 criteria: 1) phosphorylation and 2) the surrounding amino acid motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Since the amino acid sequence varies between different phosphorylation sites, certain proteins - though phosphorylated - may not be detected by the antibody. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.

General readings

Gravel A et al. Human Herpesvirus 6 immediate-early 1 protein is a sumoylated nuclear phosphoprotein colocalizing with promyelocytic leukemia protein-associated nuclear bodies. J Biol Chem 277:19679-87 (2002). PubMed PMID: 11901159
Davis PJ et al. Thyroxine promotes association of mitogen-activated protein kinase and nuclear thyroid hormone receptor (TR) and causes serine phosphorylation of TR. J Biol Chem 275:38032-9 (2000). PubMed PMID: 10982791

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Phosphoserine (incl. pos. control) antibody

Phosphoserine Detection.
Phosphoprotein Positive Control was probed with:
Lane 1: mab 1C8 (IgM), 1 μg/ml 
Lane 2: mab 4A3 (IgM), 1 μg/ml 
Lane 3: mab 4A9 (IgM), 1 μg/ml 
Lane 4: mab 4H4 (IgM), 1 μg/ml 
Lane 5: mab 7F12 (IgG), 1 μg/ml 
Lane 6: mab 16B4 (IgM), 1 μg/ml Mouse IgM 4A9 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphoserine (incl. pos. control) antibody

Phosphoserine Detection. Phosphoprotein Positive Control was probed with:
Lane 1: mab 1C8 (IgM), 1 μg/ml 
Lane 2: mab 4A3 (IgM), 1 μg/ml 
Lane 3: mab 4A9 (IgM), 1 μg/ml 
Lane 4: mab 4H4 (IgM), 1 μg/ml 
Lane 5: mab 7F12 (IgG), 1 μg/ml 
Lane 6: mab 16B4 (IgM), 1 μg/ml Mouse IgM 1C8 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphoserine (incl. pos. control) antibody

Figure 1.  Phosphoserine Detection: Phosphoprotein Positive Control was probed with lane 1: mab 1C8 (IgM), 1 μg/ml lane 2: mab 4A3 (IgM), 1 μg/ml lane 3: mab 4A9 (IgM), 1 μg/ml lane 4: mab 4H4 (IgM), 1 μg/ml lane 5: mab 7F12 (IgG), 1 μg/ml lane 6: mab 16B4 (IgM), 1 μg/ml Mouse IgM 4A3 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphoserine (incl. pos. control) antibody

Phosphoserine Detection: Phosphoprotein Positive Control was probed with LANE1: mab 1C8 (IgM), 1 μg/ml LANE2: mab 4A3 (IgM), 1 μg/ml LANE3: mab 4A9 (IgM), 1 μg/ml LANE4: mab 4H4 (IgM), 1 μg/ml LANE5: mab 7F12 (IgG), 1 μg/ml LANE6: mab 16B4 (IgM), 1 μg/ml Mouse IgM 4H4 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphoserine (incl. pos. control) antibody

Phosphoserine Detection: Phosphoprotein Positive Control was probed with: 
 Lane 1: mab 1C8 (IgM), 1 μg/ml. 
Lane 2: mab 4A3 (IgM), 1 μg/ml.
lane 3: mab 4A9 (IgM), 1 μg/ml. 
Lane 4: mab 4H4 (IgM), 1 μg/ml. 
Lane 5: mab 7F12 (IgG), 1 μg/ml. 
Lane 6: mab 16B4 (IgM), 1 μg/ml. Mouse IgG1 7F12 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphoserine (incl. pos. control) antibody

Phosphoserine Detection: Phosphoprotein Positive Control was probed with:
Lane 1: mab 1C8 (IgM), 1 μg/ml 
Lane 2: mab 4A3 (IgM), 1 μg/ml 
Lane 3: mab 4A9 (IgM), 1 μg/ml 
Lane 4: mab 4H4 (IgM), 1 μg/ml 
Lane 5: mab 7F12 (IgG), 1 μg/ml 
Lane 6: mab 16B4 (IgM), 1 μg/ml Mouse IgM 16B4 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphoserine 6x sampler kit (incl. pos. control) antibody

Figure 1. Phosphoserine Detection. Phosphoprotein Positive Control was probed with:
Lane 1: mab 1C8 (IgM), 1 μg/ml 
Lane 2: mab 4A3 (IgM), 1 μg/ml 
Lane 3: mab 4A9 (IgM), 1 μg/ml 
Lane 4: mab 4H4 (IgM), 1 μg/ml 
Lane 5: mab 7F12 (IgG), 1 μg/ml 
Lane 6: mab 16B4 (IgM), 1 μg/ml Mouse Purified Can, Hu, Ms, Rt E, IP, WB
6 x 25 µg / €580.00
  Acris Antibodies GmbH

Phosphoserine antibody

Phosphoserine Mouse IgG1 PS-53 Purified Hu, var. WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Phosphoserine antibody

Phosphoserine Rabbit IgG HRP E, WB
0.4 ml / €450.00
  Acris Antibodies GmbH

Phosphoserine antibody

Western blot analysis of the phosphorylated proteins with UV-treated cell lysates Mouse spleen cell. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1μM okadaic acid). Rabbit IgG Aff - Purified C, E, IP, WB
0.4 ml / €390.00
  Acris Antibodies GmbH

Phosphoserine antibody

The cell lysate derived from EGF-stimulated A-431was resolved onto 12% SDS-PAGE and immunoblotted by Phosphoserine monoclonal antibody, clone A8G9 ( Cat # MAB2545 ) at 1 : 500 ( lane A ) ; or pre-incubated by Phosphoserine ( land B ) or pre-incubated by phosphotyrosine ( lnae C ). A panel of phosphorylated proteins was observed. Mouse IgG A8G9 Hu, Ms, Rt E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Phosphoserine antibody

Phospho Serine polyclonal antibody ( Cat # PAB10351 ) is shown to detect phosphorylated proteins present in UV-treated mouse spleen cell lysates ( top panel ). Rabbit IgG C, E, F, IP, R
0.1 mg / €560.00
  Abnova Taiwan Corp.

Phosphoserine antibody

Phosphoserine Rabbit IgG E, IP, WB
0.1 mg / €730.00
  Abnova Taiwan Corp.

Phosphoserine antibody

Western Blot: Phosphoserine Antibody [NB100-1953] Rabbit Aff - Purified E, IP, P, WB
0.4 ml / €450.00
  Novus Biologicals Inc.

Phosphoserine antibody

Western Blot: Phosphoserine Antibody [NB100-1981] - Western blot analysis of human MMRU melanoma cells probed with Phosphoserine polyclonal antibody. Top (from the left): MMRU cells treated with 0 (A), 50 (B), 200 (C), 400 (D) l long wavelength UV, or 0.1uM of okadaic acid (E) Bottom: Same samples, except probed with b-actin antibody Rabbit HRP E, WB
0.2 ml / €570.00
  Novus Biologicals Inc.

Phosphoserine antibody

Phosphoserine Rabbit IgG Purified All Species E, IP, WB
50 µl / €400.00
  Novus Biologicals Inc.

Phosphoserine antibody

Western blot: Phosphoserine antibody (PSR-45) [600-405] - Mouse IgG1 PSR-45 Ascites Hu, Rt E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Phosphoserine / Phosphothreonine / Phosphotyrosine antibody

Human breast ca. stained with Anti-phospho pan antibody Mouse IgG1 SPM101 Purified All Species ICC/IF, IP, P, WB
0.5 ml / €300.00
  Acris Antibodies GmbH

Phosphoserine / Phosphothreonine / Phosphotyrosine antibody

Human breast ca. stained with Anti-phospho pan antibody Mouse IgG1 SPM101 Purified All Species ICC/IF, IP, P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

Phosphoserine / Phosphothreonine / Phosphotyrosine antibody

Human breast ca. stained with Anti-phospho pan antibody Mouse IgG1 SPM101 Purified All Species ICC/IF, IP, P, WB
1 ml / €320.00
  Acris Antibodies GmbH

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