Phosphothreonine antibody

Principal name

Phosphothreonine antibody

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), All Species

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C), Radioimmunoassay (R), Flow Cytometry (F)

Background of Phosphothreonine antibody

Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation. Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr. An understanding of transformation by oncogenes and mitogenic processes of growth factors depends on the identification of their substrate and a subsequent determination of how phosphorylation affects their properties. Studies on the role of phosphorylated proteins have been hampered by their low abundance and the problem of distinguishing the various types of phosphorylated proteins. The most common procedure is to label intact cells or small tissue fragments with 32P and subsequently to isolate 32P labeled proteins by conventional biochemical methods. In order to identify the specific amino acids that undergo phosphorylation, additional long and tedious procedures for phosphoamino acid analysis are required. Immunoblotting of cellular proteins with antibodies directed against phosphoamino acids is advantageous as it does not involve 32P labeling, and can therefore be employed to monitor alterations in phosphorylation of specific proteins as they occur in intact organs or the whole animal. Indeed, mono and polyclonal antibodies directed against phosphorylated residues have been generated and found useful as analytical and preparative tools because they enable the rapid identification, quantification and immunoaffinity isolation of phosphorylated cellular proteins.

General readings

Frackelton, AR, et al. (1983) Characterization and use of monoclonal antibodies for isolation of phosphotyrosyl proteins from retrovirustransformed cells and growth factor-stimulated cells. Mol. Cell. Biol. 3:1343.
Edleman, AM, et al. (1987) Protein serine/threonine kinases. Annu. Rev. Biochem. 56:567.
Hunter, T. (1987)A thousand and one protein kinases. Cell 50:823.
Sengupta, A, et al, (1988)Identification and subcellular localization of proteins that are rapidly phosphorylated in tyrosine in response to colony-stimulating factor 1. Proc. Natl. Acad. Sci. USA 85:8062.
Heffetz, D, et al, (1991)Generation and use of antibodies to phosphothreonine. Methods in Enzymology 210:44.
Hunter, T. (1991)Protein kinase classification. Methods in Enzymology 200:3.
Rintamaki, E. et al, (1997) Phosphorylation of light-harvesting complex II and photosystem II core proteins shows irradiance-dependent regulation in vivo. Application of phosphothreonine antibodies to analysis of thylakoid phosphoproteins. J. Biol. Chem. 272(48): 30476-30482.
Jin, DY, et al, (1998) Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1. Cell. 93:81-91.
Reilein A.R., et al, (1998) Regulation of organelle movement in melanophores by protein kinase A (PKA), protein kinase C (PKC), and protein phosphatase 2A (PP2A). J. Cell Biol. 142(3):803-813.
Fleming, I.N., et al, (1999) Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation. J. Biol. Chem. 274(18):12753-12758.

14 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Phosphothreonine (incl. pos. control) antibody

Figure 1. Phosphothreonine: A431 cell Lysates probed with lane1: mab 14B3 (IgG) 1 μg/ml, lane2: mab 4D11 (IgM), 1 μg/ml, lane3: mab 1E11 (IgG), 1 μg/ml. Mouse IgG1 1E11 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphothreonine (incl. pos. control) antibody

Phosphothreonine: A431 cell Lysates probed with:
Lane 1: mab 14B3 (IgG), 1 μg/ml 
Lane 2: mab 4D11 (IgM), 1 μg/ml 
Lane 3: mab 1E11 (IgG), 1 μg/ml Mouse IgM 4D11 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphothreonine (incl. pos. control) antibody

Figure 1. Phosphothreonine Detection: A431 cell Lysates probed with Lane 1: mab 14B3 (IgG), 1 μg/ml. Lane 2: mab 4D11 (IgM), 1 μg/ml. Lane 3: mab 1E11 (IgG), 1 μg/ml. Mouse IgG1 14B3 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphothreonine 3x sampler kit (incl. pos. control) antibody

Phosphothreonine Mouse Purified Can, Hu, Ms, Rt E, IP, WB
3 x 25 µg / €370.00
  Acris Antibodies GmbH

Phosphothreonine antibody

ELISA results of Mab anti-phosphothreonine antibody
tested against BSA conjugates of pT, pY and pS. Each
well was coated with 0.1 μg of conjugate. The starting
dilution of antibody was 1:1000 and each point on the
x-axis represents a 2-fold dilution. HRP conjugated
Gt-a-Mouse IgG (H&L) and TMB substrate was used for
detection. Mouse IgG1 18F6 Ascites E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Phosphothreonine antibody

Phosphothreonine Rabbit IgG HRP E, WB
0.4 ml / €450.00
  Acris Antibodies GmbH

Phosphothreonine antibody

Western blot analysis of Mouse brain extract cells with Rabbit anti-Phosphothreonine Antibody Cat.-No AP03034SU-N. Left: Treated with Vanadium. Right: Non-treated. Rabbit IgG Purified E, IP, WB
0.4 ml / €390.00
  Acris Antibodies GmbH

Phosphothreonine antibody

Phosphothreonine Rabbit IgG Purified E, IP, WB
50 µg / €310.00
  Acris Antibodies GmbH

Phosphothreonine antibody

Phosphothreonine Rabbit Serum C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Phosphothreonine antibody

Figure 2. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate after EGF stimulation of A431 cells. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore Inc. A 1:1,000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2,500 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc. Mouse IgG1 18F6 Ascites E, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

Phosphothreonine antibody

Phospho Threonine monoclonal antibody, clone 18F6 ( Cat # MAB2001 ) is shown to detect threonine phosphorylation of proteins in a lysate from A-431 cells stimulated with EGF.
Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc.
A 1 : 1,000 dilution of Phospho Threonine monoclonal antibody, clone 18F6 ( Cat # MAB2001 ) is used for 30 min followed by detection using a 1 : 2,500 dilution of IRDye® 800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR.
Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
0.1 ml / €560.00
  Abnova Taiwan Corp.

Phosphothreonine antibody

Phospho Threonine polyclonal antibody ( Cat # PAB10352 ) is shown to detect phosphorylated proteins present in mouse spleen cell lysates. Left Lane: extract of spleen from mouse treated with vanadium. Right lane: extract of spleen from untreated mouse. Rabbit IgG C, E, F, IP, R
0.1 mg / €560.00
  Abnova Taiwan Corp.

Phosphothreonine antibody

Phosphothreonine Rabbit Serum E, IP, WB
0.4 ml / €450.00
  Novus Biologicals Inc.

Phosphothreonine antibody

Phosphothreonine Mouse IgG1 18F6 Ascites All Species E, WB
50 µl / €310.00
  Novus Biologicals Inc.

14 Item(s)

per page
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