Phosphotyrosine antibody

Principal name

Phosphotyrosine antibody

Ncbi ID

5874

Available reactivities

All Species, Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Broad, Br, Chk (Chicken)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P), Radioimmunoassay (R)

Background of Phosphotyrosine antibody

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the nucleus. Phosphorylated epitopes may serve as docking sites for the assembley of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions.
Modification of proteins on tyrosine residues is mediated by protein tyrosin kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via the interaction of phosphotyrosine residues with SH2 or PTB domains.
Antibodies direct against phosphorylated epitopes recognize the phosphorylated amino acid in the context of the surrounding amino acid sequence. Recognition is therefore dependent on 2 criteria: 1) phosphorylation and 2) the surrounding amino acid motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Since the amino acid sequence varies between different phosphorylation sites, certain proteins - though phosphorylated - may not be detected by the antibody.
Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity.

General readings

General / background references:Sarmay G et al. Protein-tyrosine kinase activity tightly associated with human type II Fc gamma receptors. Proc Natl Acad Sci U S A 91:4140-4 (1994). PubMed PMID: 8183883
Far DF et al. Immunofluorescent quantification of tyrosine phosphorylation of cellular proteins in whole cells by flow cytometry. Cytometry 15:327-34 (1994). PubMed PMID: 7517816
Barry ST & Critchley DR The RhoA-dependent assembly of focal adhesions in Swiss 3T3 cells is associated with increased tyrosine phosphorylation and the recruitment of both pp125FAK and protein kinase C-delta to focal adhesions. J Cell Sci 107 (Pt 7):2033-45 (1994). PubMed PMID: 7527052
Arad-Dann H et al. Immunohistochemistry of phosphotyrosine residues: identification of distinct intracellular patterns in epithelial and steroidogenic tissues. J Histochem Cytochem 41:513-9 (1993). PubMed PMID: 7680679
Dvir A et al. The inhibition of EGF-dependent proliferation of keratinocytes by tyrphostin tyrosine kinase blockers. J Cell Biol 113:857-65 (1991). PubMed PMID: 1709168

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Mouse IgG1 2A5 Biotin Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Detection: Lysates of pervanadate-treated A431 cells were probed with Lane 1: mab 2A5 (IgG), 1μg/ml 
Lane 2: mab 2C8 (IgG), 1μg/ml 
Lane 3: mab 3B12 (IgG), 1μg/ml 
Lane 4: mab 9H8 (IgG), 1μg/ml 
Lane 5: mab 16F4 (IgG), 1µg/ml 
Lane 6: mab 9F1 (IgG), 1µg/ml Mouse IgG1 2A5 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Detection Lysates of pervanadate-treated A431 cells were probed with LANE1: mab 2A5 (IgG), 1μg/ml LANE2: mab 2C8 (IgG), 1μg/ml LANE3: mab 3B12 (IgG), 1μg/ml LANE4: mab 9H8 (IgG), 1μg/ml LANE5: mab 16F4 (IgG), 1µg/ml LANE6: mab 9F1 (IgG), 1µg/ml Mouse IgG1 2C8 Biotin Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Mouse IgG1 2C8 FITC Can, Hu, Ms, Rt F
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Detection Lysates of pervanadate-treated A431 cells were probed with LANE1: mab 2A5 (IgG), 1μg/ml LANE2: mab 2C8 (IgG), 1μg/ml LANE3: mab 3B12 (IgG), 1μg/ml LANE4: mab 9H8 (IgG), 1μg/ml LANE5: mab 16F4 (IgG), 1µg/ml LANE6: mab 9F1 (IgG), 1µg/ml Mouse IgG1 2C8 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Mouse IgG1 3B12 Biotin Can, Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Detection Lysates of pervanadate-treated A431 cells were probed with LANE1: mab 2A5 (IgG), 1μg/ml LANE2: mab 2C8 (IgG), 1μg/ml LANE3: mab 3B12 (IgG), 1μg/ml LANE4: mab 9H8 (IgG), 1μg/ml LANE5: mab 16F4 (IgG), 1µg/ml LANE6: mab 9F1 (IgG), 1µg/ml Mouse IgG1 3B12 Purified Can, Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Phosphotyrosine (pYpY Motif) (incl. pos. control) antibody

Phosphotyrosine Mouse IgG1 9F1 Biotin Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphotyrosine (pYpY Motif) (incl. pos. control) antibody

Phosphotyrosine Detection: Lysates of pervanadate-treated A431 cells were probed with Lane 1: mab 2A5 (IgG), 1μg/ml 
Lane 2: mab 2C8 (IgG), 1μg/ml 
Lane 3: mab 3B12 (IgG), 1μg/ml 
Lane 4: mab 9H8 (IgG), 1μg/ml 
Lane 5: mab 16F4 (IgG), 1µg/ml 
Lane 6: mab 9F1 (IgG), 1µg/ml Mouse IgG1 9F1 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Mouse IgG1 9H8 Biotin Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Detection: Lysates of pervanadate-treated A431 cells were probed wit:
Lane 1: mab 2A5 (IgG), 1µg/ml
Lane 2: mab 2C8 (IgG), 1µg/ml
Lane 3: mab 3B12 (IgG), 1µg/ml
Lane 4: mab 9H8 (IgG), 1µg/ml
Lane 5: mab 16F4 (IgG), 1µg/ml
Lane 6: mab 9F1 (IgG), 1µg/ml Mouse IgG1 9H8 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Mouse IgG1 16F4 Biotin Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Phosphotyrosine (incl. pos. control) antibody

Phosphotyrosine Detection Lysates of pervanadate-treated A431 cells were probed with LANE1: mab 2A5 (IgG), 1μg/ml LANE2: mab 2C8 (IgG), 1μg/ml LANE3: mab 3B12 (IgG), 1μg/ml LANE4: mab 9H8 (IgG), 1μg/ml LANE5: mab 16F4 (IgG), 1µg/ml LANE6: mab 9F1 (IgG), 1µg/ml Mouse IgG1 16F4 Purified Can, Hu, Ms, Rt E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Phosphotyrosine 7x sampler kit (incl. pos. control) antibody

Phosphotyrosine Detection:  Lysates of pervanadate-treated A431 cells were probed with:  lane 1: mab 2A5 (1 μg/ml), lane 2: mab 2C8 (1 μg/ml), lane 3: mab 3B12 (1 μg/ml), lane 4: mab 9H8 (1 μg/ml), lane 5: mab 16F4 (1 μg/ml), lane 6: mab 9F1 (1 μg/ml). Mouse Purified Can, Hu, Ms, Rt
7 x 25 µg / €520.00
  Acris Antibodies GmbH

Phosphotyrosine antibody

Phosphotyrosine Mouse IgG2b PY20 Agarose C, E, IP, WB
1 ml / €660.00
  Acris Antibodies GmbH

Phosphotyrosine antibody

Phosphotyrosine Mouse IgG2b PY20 Agarose C, E, IP, WB
0.5 ml / €370.00
  Acris Antibodies GmbH

Phosphotyrosine antibody

Phosphotyrosine Mouse IgG2b PY20 Agarose C, E, IP, WB
0.2 ml / €200.00
  Acris Antibodies GmbH

Phosphotyrosine antibody

Phosphotyrosine Mouse IgG2b PY20 Biotin C, E, IP, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

Phosphotyrosine antibody

Phosphotyrosine Mouse IgG2b PY20 Biotin C, E, IP, WB
50 µg / €190.00
  Acris Antibodies GmbH

Phosphotyrosine antibody

Phosphotyrosine Mouse IgG2b PY20 FITC All Species F
0.5 mg / €340.00
  Acris Antibodies GmbH

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