PHPT1 antibody
Principal name
PHPT1 antibody
Alternative names for PHPT1 antibody
Phosphohistidine phosphatase 1, PHP14, HSPC141, 14 kDa phosphohistidine phosphatase, Protein janus-A homolog
Ncbi ID
NP_054891, 9606, NP_001129333, NP_001129333.1, NP_083569.1, NM_029293, NM_001135861
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of PHPT1 antibody
- Kit Component:
- - KN313236G1, Phpt1 gRNA vector 1 in pCas-Guide vector
- KN313236G2, Phpt1 gRNA vector 2 in pCas-Guide vector
- KN313236D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Srivastava S., et al. (2008) Proc Natl Acad Sci U S A. 105(38):14442-6.
van Vlierberghe P., et al. (2006) Leukemia. 20(7):1245-53.
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 polyclonal antibody ( Cat # PAB2560 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of PHPT1 ( arrow ) using rabbit PHPT1 polyclonal antibody ( Cat # PAB2560 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PHPT1 gene ( Lane 2 ) ( Origene Technologies ) .
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 polyclonal antibody ( Cat # PAB2561 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of PHPT1 ( arrow ) using rabbit PHPT1 polyclonal antibody ( Cat # PAB2561 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the PHPT1 gene ( Lane 2 ) ( Origene Technologies ) .
Recombinant human PHPT1, 1-125 aa, His-tagged
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of PHPT1 (arrow) using rabbit polyclonal PHPT1 Antibody (Human C-term) (Cat#.AP2127b).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 Antibody (N-term) (Cat.#TA302276), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of PHPT1 (arrow) using rabbit polyclonal PHPT1 Antibody (Human N-term) (Cat#.TA302277).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Predicted band size: 14 kDa . Positive control: MCF7 cell and kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lane1: MCF7 cell lysate Lane2: Kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Western blot analysis of extracts of various cell lines, using PHPT1 antibody.
Immunofluorescence analysis of HeLa cell using PHPT1 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-Phpt1 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Brain
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302276 | PHPT1 (C-term) antibody |
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| Rabbit Polyclonal Antibody against PHPT1 (C-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA302277 | PHPT1 (N-term) antibody |
||||||
| Rabbit Polyclonal Antibody against PHPT1 (N-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA323874 | PHPT1 antibody |
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| Anti-PHPT1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326938 | PHPT1 antibody |
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| PHPT1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA334498 | PHPT1 antibody |
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| Rabbit Polyclonal Anti-Phpt1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, GP, Hu, Ms, Rb, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
