PIAS1 antibody

Principal name

PIAS1 antibody

Alternative names for PIAS1 antibody

PIAS-1, DDXBP1, Gu-binding protein, RNA helicase II-binding protein, DEAD/H box-binding protein 1, Protein inhibitor of activated STAT protein 1, E3 SUMO-protein ligase PIAS1

SwissProt ID

O75925 (Human), O88907 (Mouse)

Gene ID

8554 (PIAS1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PIAS1 antibody

PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (sigl transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the D binding activity, binds to Gu protein/R helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptiol coregulator and may have a role in AR initiation and maintence of spermatogenesis.This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (sigl transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the D binding activity, binds to Gu protein/R helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptiol coregulator and may have a role in AR initiation and maintence of spermatogenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIAS1 antibody

HEK293 cell extracts were prepared and immunoprecipitated with TA302212 (left lane alpha-PIAS1 IP) or a control antibody (right lane Control IP), followed by Western blotting with TA302212. Taken from Polyakova o, Borman S, Grimley R, Vamathevan J, Hayes B. et al. (2012) Identification of Novel interacting Partners of Sirtuin6. Plos ONE 7 (12): e51555. doi: 10.1371/journal.pone. 0051555 Rabbit Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS1 (C-term) antibody

Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (C-term) (Cat.#TA302213). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

Western blot analysis of PIAS1 in human kidney tissue lysate with PIAS1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

Western blot analysis of extracts from MDA-MB-435 cells, using PIAS1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

WB Suggested Anti-PIAS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

Lanes: Lane 1: Untreated human U251 lysate; Lane 2: IL-1beta treated human U251 lysate; Primary Antibody Dilution: 1: 500; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 500; Gene Name: PIAS1; Submitted by: Anonymous; Rabbit IgG Purified Bov, Can, Eq, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

Western blot analysis on MDA-MB-435 cell lysate using PIAS1 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

Immunohistochemistry (IHC) analyzes of PIAS1 antibody (Cat.-No.: AP06452PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PIAS1 (C-term) antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PIAS1 Control Peptide

PIAS1
50 µg / €160.00
  Acris Antibodies GmbH

PIAS1 antibody

Western Blot analysis of PIAS1 expression in transfected 293T cell line (H00008554-T01) by PIAS1 MaxPab polyclonal antibody.

Lane 1: PIAS1 transfected lysate(71.61 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PIAS1 antibody

Immunoprecipitation of PIAS1 transfected lysate using anti-PIAS1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PIAS1 MaxPab mouse polyclonal antibody (B01) (H00008554-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

PIAS1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and PIAS1 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PIAS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2b 4A4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PIAS1 antibody

Formalin-fixed and paraffin-embedded human hepatocellular cancer tissue reacted with PIAS1 polyclonal antibody ( Cat # PAB1850 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PIAS1 antibody

Formalin-fixed and paraffin-embedded human hepatocellular cancer tissue reacted with PIAS1 polyclonal antibody ( Cat # PAB1851 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PIAS1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

PIAS1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

PIAS1 antibody

Human Placenta; WB Suggested Anti-PIAS1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; PIAS1 antibody - N-terminal region (ARP39231_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

PIAS1 antibody

Human U251; . Lanes: Lane 1: Untreated human U251 lysate. Lane 2: IL-1beta treated human U251 lysate. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:500. Gene Name: PIAS1. Submitted by: Anonymous .; PIAS1 antibody - middle region (ARP39232_P050) in Human U251 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

PIAS1 antibody

Immunohistochemistry-Paraffin: PIAS1 Antibody [NB100-92097] - Paraffin-embedded human lung carcinoma tissue. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

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