PIAS1 antibody

Principal name

PIAS1 antibody

Alternative names for PIAS1 antibody

PIAS-1, DDXBP1, Gu-binding protein, RNA helicase II-binding protein, DEAD/H box-binding protein 1, Protein inhibitor of activated STAT protein 1, E3 SUMO-protein ligase PIAS1

SwissProt ID

O75925 (Human), O88907 (Mouse)

Gene ID

8554 (PIAS1), 56469 (Pias1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of PIAS1 antibody

PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (sigl transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the D binding activity, binds to Gu protein/R helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptiol coregulator and may have a role in AR initiation and maintence of spermatogenesis.This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (sigl transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the D binding activity, binds to Gu protein/R helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptiol coregulator and may have a role in AR initiation and maintence of spermatogenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIAS1 antibody

  Rabbit Polyclonal Antibody against PIAS1 (N-term)  
HEK293 cell extracts were prepared and immunoprecipitated with TA302212 (left lane alpha-PIAS1 IP) or a control antibody (right lane Control IP), followed by Western blotting with TA302212. Taken from Polyakova o, Borman S, Grimley R, Vamathevan J, Hayes B. et al. (2012) Identification of Novel interacting Partners of Sirtuin6. Plos ONE 7 (12): e51555. doi: 10.1371/journal.pone. 0051555 Rabbit Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS1 (C-term) antibody

  Rabbit Polyclonal Antibody against PIAS1 (C-term)  
Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (C-term) (Cat.#TA302213). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

  Rabbit Polyclonal PIAS1 Antibody  
Western blot analysis of PIAS1 in human kidney tissue lysate with PIAS1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

  Rabbit polyclonal PIAS1 antibody  
Western blot analysis of extracts from MDA-MB-435 cells, using PIAS1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

  Rabbit Polyclonal Anti-PIAS1 Antibody  
WB Suggested Anti-PIAS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

  Rabbit Polyclonal Anti-PIAS1 Antibody  
Lanes: Lane 1: Untreated human U251 lysate; Lane 2: IL-1beta treated human U251 lysate; Primary Antibody Dilution: 1: 500; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 500; Gene Name: PIAS1; Submitted by: Anonymous; Rabbit IgG Purified Bov, Can, Eq, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

  Rabbit Polyclonal Anti-PIAS1 Antibody  
Western blot analysis on MDA-MB-435 cell lysate using PIAS1 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

  Rabbit Polyclonal Anti-PIAS1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Human testis tissue, human placenta tissue, Primary antibody: (PIAS1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIAS1 antibody

Immunohistochemistry (IHC) analyzes of PIAS1 antibody (Cat.-No.: AP06452PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PIAS1 (C-term) antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

PIAS1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €230.00
  Assay Biotech

PIAS1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Pias1 - mouse gene knockout kit via CRISPR

Pias1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pias1 - mouse gene knockout kit via CRISPR

Pias1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pias1 - mouse gene knockout kit via CRISPR

Pias1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pias1 - mouse gene knockout kit via CRISPR

Pias1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

PIAS1 overexpression lysate

PIAS1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

PIAS1-knockout Cell lysate

PIAS1-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PIAS1 (mGFP-tagged) - Human protein inhibitor of activated STAT, 1 (PIAS1), 200 uL, >10^7 TU/mL

Lenti ORF particles, PIAS1 (mGFP-tagged) - Human protein inhibitor of activated STAT, 1 (PIAS1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PIAS1 (Myc-DDK tagged) - Human protein inhibitor of activated STAT, 1 (PIAS1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, PIAS1 (Myc-DDK tagged) - Human protein inhibitor of activated STAT, 1 (PIAS1) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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