PIAS2 antibody

Principal name

PIAS2 antibody

Alternative names for PIAS2 antibody

PIASX, ARIP3, DAB2-interacting protein, DIP, Miz1, PIAS-NY protein, E3 SUMO-protein ligase PIAS2, Androgen receptor-interacting protein 3, Msx-interacting zinc finger protein, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2

SwissProt ID

O75928 (Human), Q6AZ28 (Rat), Q8C5D8 (Mouse)

Gene ID

9063 (PIAS2), 17344 (Pias2)

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Frozen Sections (C)

Background of PIAS2 antibody

PIAS2 functions as an E3 type small ubiquitin like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO mediated repression of ELK1 transactivation activity. Isoform PIAS2 beta, but not isoform PIAS2 alpha, promotes MDM2 sumoylation. Isoform PIAS2 beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2 alpha. This product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application.

General readings

van den Akker E, Ano S, Shih HM, Wang LC, Pironin M, Palvimo JJ, Kotaja N, Kirsh O, Dejean A, Ghysdael J. FLI-1 functionally interacts with PIASxalpha, a member of the PIAS E3 SUMO ligase family. J Biol Chem. 2005 Nov 11;280(45):38035-46. Epub 2005 Sep 7. PMID: 16148010

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIAS2 antibody

WB Suggested Anti-PIAS2 Antibody Titration: 0.6 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS2 antibody

Immunofluorescence of purified MaxPab antibody to PIAS2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu, Ms E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

PIAS2 antibody

Immunofluorescence of monoclonal antibody to PIAS2 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG 1F7 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PIAS2 antibody

Human 721_B; Host: Rabbit. Target Name: PIAS2. Sample Tissue: 721_B. Antibody Dilution: 1.0ug/ml. PIAS2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; PIAS2 antibody - C-terminal region (ARP35735_T100) in Human 721_B cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms WB
0.1 mg / €310.00
  AVIVA Systems Biology

PIAS2 (185-196) antibody

Antibody (AP23730PU-N) (1µg/ml) staining of Human Peripheral Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

PIAS2 antibody

TA305708 (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Can, Hu, Ms, Rt P
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS2 antibody

Western blot analysis of extracts from COS7 cells, using PIAS2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIAS2 (N-term) antibody

Western blot analysis of PIAS2 in rat brain tissue lysate with PIAS2 antibody at (A) 1 and (B) 2 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS2 antibody

Western blot analysis of extracts of various cell lines, using PIAS2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS2 antibody

WB Suggested Anti-PIAS2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIAS2 antibody

WB Suggested Anti-PIAS2 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1:2500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS2 antibody

WB Suggested Anti-Pias2 Antibody Titration: 0.2-1 ug/ml; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIAS2 antibody

Western blot analysis on COS7 cell lysate using PIAS2 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIAS2 (RC212659, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIAS2.(1:2000) Mouse IgG1 OTI2B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

PIAS2 antibody

Western blot (WB) analysis of PIAS2 antibody (Cat.-No.: AP06453PU-N) in extracts from COS7 cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PIAS2 (PIASx1/2) (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PIAS2 (431-445) antibody

Human Testis (formalin-fixed, paraffin-embedded) stained with PIAS2 antibody AP22538PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Prim, Rb, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

PIAS2 control peptide

PIAS2
50 µg / €160.00
  Acris Antibodies GmbH

PIAS2 antibody

PIAS2 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

PIAS2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PIAS2 and CDKN2B. HeLa cells were stained with anti-PIAS2 rabbit purified polyclonal 1:1200 and anti-CDKN2B mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

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