PIAS4 antibody

Principal name

PIAS4 antibody

Alternative names for PIAS4 antibody

PIASG, PIAS-gamma, PIASy, E3 SUMO-protein ligase PIAS4, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma

SwissProt ID

Q8N2W9 (Human), Q9JM05 (Mouse)

Gene ID

51588 (PIAS4), 59004 (Pias4)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of PIAS4 antibody

The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

General readings

Zhang,J., et al., (2004) FEBS Lett. 570 (1-3), 97-101

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PIAS4 antibody

Western blot analysis of extracts from Jurkat cells, using PIAS4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

PIAS4 (N-term) antibody

Western blot analysis of PIAS4 in rat brain tissue lysate with PIAS4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS4 antibody

WB Suggested Anti-PIAS4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

PIAS4 antibody

Western blot analysis of PIAS4 expression in Jurkat cell extract Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PIAS4 antibody

Immunohistochemistry (IHC) analyzes of PIAS4 antibody (Cat.-No.: AP06455PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

PIAS4 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PIAS4 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PIAS4 control peptide

PIAS4
50 µg / €160.00
  Acris Antibodies GmbH

PIAS4 antibody

PIAS4 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

PIAS4 antibody

Western blot analysis of PIAS4 in A ) human liver, B ) mouse liver, C ) rat liver and D ) human Jurkat lysate using PIAS4 polyclonal antibody ( Cat # PAB0167 ) at 5 µg/ml. Rabbit Hu ICC/IF, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PIAS4 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PIAS4 polyclonal antibody ( Cat # PAB2257 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, IP, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

PIAS4 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PIAS4 polyclonal antibody ( Cat # PAB2258 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PIAS4 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

PIAS4 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

PIAS4 antibody

Human Jurkat; WB Suggested Anti-PIAS4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; PIAS4 antibody - N-terminal region (ARP33011_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Pias4 - mouse gene knockout kit via CRISPR

Pias4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pias4 - mouse gene knockout kit via CRISPR

Pias4 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pias4 - mouse gene knockout kit via CRISPR

Pias4 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pias4 - mouse gene knockout kit via CRISPR

Pias4 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

PIAS4

PIAS4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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