PICK1 antibody

Principal name

PICK1 antibody

Alternative names for PICK1 antibody

PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein

SwissProt ID

Q2T9M1 (Bovin), Q62083 (Mouse), Q9EP80 (Rat), Q9NRD5 (Human)

Gene ID

9463 (PICK1), 84591 (Pick1), 18693

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Can (Canine), Por (Porcine), Bov (Bovine)

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of PICK1 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). PRKCA-binding protein is a protein that is encoded by the Pick1 gene (3, 4). The protein encoded by this gene contains a PSD domain, which interacts with protein kinase C, alpha. The protein functions as an adaptor that binds to and organizes subcellular localization of a variety of membrane proteins. It has been shown to interact with multiple glutamate receptor subtypes, monoamine plasma membrane transporters, and non-voltage gated sodium channels. This protein has also been found to act as an anchoring protein that specifically targets PRKCA to mitochondria in a ligand-specific manner (5).

General readings

General / background references:Perez JL et al. PICK1 targets activated protein kinase Calpha to AMPA receptor clusters in spines of hippocampal neurons and reduces surface levels of the AMPA-type glutamate receptor subunit 2. J Neurosci 21:5417-28 (2001). PubMed PMID: 11466413
Staudinger J et al. Specific interaction of the PDZ domain protein PICK1 with the COOH terminus of protein kinase C-alpha. J Biol Chem 272:32019-24 (1997). PubMed PMID: 9405395

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

PICK1 antibody

immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe. Mouse IgG2a L20/8 Supernatant Hu, Ms, Rt C, ICC/IF, IP, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

PICK1 antibody

immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with L20/8 (left) or N52A/42 (right) TC supe. Mouse IgG2a L20/8 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

PICK1 antibody

TA303155 (1µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

PICK1 antibody

TA303155 (1µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PICK1 antibody

IHC analysis of Pick1 in frozen sections of mouse brain extract using TA309463. Mouse IgG2a S20-8 Purified Hu, Ms, Rt C, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

PICK1 antibody

Western blot analysis of extracts of various cell lines, using PICK1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

PICK1 (N-term) antibody

The anti-PRKCABP Pab is used in Western blot to detect PRKCABP in Y79 cell lysate. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PICK1 (C-term) antibody

Western blot analysis of anti-PRKCABP Antibody (C-term) in K562 cell line lysates (35ug/lane). PRKCABP(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

PICK1 antibody

PRKCABP polyclonal antibody (A01), Lot # 050921JC01 Western Blot analysis of PRKCABP expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

PICK1 antibody

Western Blot analysis of PICK1 expression in transfected 293T cell line (H00009463-T01) by PICK1 MaxPab polyclonal antibody.

Lane 1: PRKCABP transfected lysate(45.65 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

PICK1 antibody

PICK1 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

PICK1 antibody

Immunoprecipitation of PICK1 transfected lysate using anti-PICK1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PRKCABP MaxPab mouse polyclonal antibody (B01) (H00009463-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

PICK1 antibody

PICK1 Mouse IgG2a 3G5 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

PICK1 antibody

The PICK1 polyclonal antibody ( Cat # PAB4631 ) is used in Western blot to detect PICK1 in Y-79 cell lysate . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PICK1 antibody

The PICK1 polyclonal antibody ( Cat # PAB4632 ) is used in Western blot to detect PICK1 in mouse pancreas tissue lysate . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

PICK1 antibody

PICK1 polyclonal antibody ( Cat # PAB7130 ) ( 1 µg/ml ) staining of mouse brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

PICK1 antibody

Western Blot: PICK1 Antibody [NB100-41403] - NB100-41403 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat IgG Aff - Purified Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PICK1 antibody

Figure 1 illustrates immunolocalization of PICK1 in CV1 cells transfected with both PICK1 and mGluR7a using NB120-3420. Figure 2 illustrates Western blot detection of PICK1 from AtT20 cell lysate using Nb120-3420. Rabbit IgG Aff - Purified Ms, Rt ICC/IF, IP, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

PICK1 (C-term) antibody

Immunohistochemistry: PICK1 Antibody [NBP1-42829] - Immunohistochemical analysis of PICK1 on mouse bladder using NBP1-42829. Chicken Aff - Purified Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

PICK1 antibody

PICK1 Chicken Aff - Purified Ms, Rt IP, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

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